Stem Cell Tracking with Nanoparticle-Based Ultrasound Contrast Agents

Chen, Fang; Jokerst, Jesse V.

doi:10.1007/978-1-0716-0364-2_13

Cited by 7 publications

(7 citation statements)

References 22 publications

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“…[49][50][51][52] They are promising ultrasound contrast agents for cell tracking because of their small size, high cellular uptake, and high acoustic impedance, which is very different from that of cells. [53,54] Positively charged 1 µm microbubbles have been reported to be internalized by neuroprogenitor cells, and these labeled cells can be detected in vivo for days. [23] However, the mechanism by which bubbles interact with cells is not clear because the dynamic geometries and configurations of the bubbles are complex.…”

Section: Discussionmentioning

confidence: 99%

Magnetic Nanobubble Mechanical Stress Induces the Piezo1‐Ca²⁺‐BMP2/Smad Pathway to Modulate Neural Stem Cell Fate and MRI/Ultrasound Dual Imaging Surveillance for Ischemic Stroke

Zhang

Lou

et al. 2022

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Neural stem cells (NSCs) are used to treat various nervous system diseases because of their self‐renewal ability and multidirectional differentiation potential. However, an insufficient ability to track their migration in vivo and poor control over their survival and differentiation efficiency are two major critical challenges for clinical application. Here, it is shown that when magnetic nanobubbles (MNBs), which are assembled from magnetic nanoparticles, are internalized by NSCs, intramembrane volumetric oscillation of the MNBs induces an increase in intracellular hydrostatic pressure and cytoskeleton force, resulting in the activation of the Piezo1‐Ca2+ mechanosensory channel. This subsequently triggers the BMP2/Smad biochemical signaling pathway, leading to differentiation of NSCs into the neuronal phenotype. Signaling through the Piezo1‐Ca2+‐BMP2/Smad pathway can be further accelerated by application of an external shear stress force using low‐intensity pulsed ultrasound. More importantly, magnetic resonance imaging and ultrasound imaging surveillance of NSCs based on MNB labeling can be leveraged to provide NSC therapeutic outcomes. Both the in vitro and in vivo findings demonstrate that a bubble nanostructure‐induced physical force can modulate and control the mechanical signaling pathway regulating stem cell development.

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Section: Discussionmentioning

confidence: 99%

Magnetic Nanobubble Mechanical Stress Induces the Piezo1‐Ca²⁺‐BMP2/Smad Pathway to Modulate Neural Stem Cell Fate and MRI/Ultrasound Dual Imaging Surveillance for Ischemic Stroke

Zhang

Lou

et al. 2022

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“…Traditionally, histological analysis has been performed to monitor therapeutic cells, but this method is invasive and requires multiple tissue biopsies [ 45 ]. NP-based imaging technologies are noninvasive methods to track and visualize the fate and function of transplanted cells [ 44 , 45 , 48 , 49 ]. In stem cell transplantation, to monitor the survival, location, migration, and differentiation of the cells in therapeutic processes, NP-based labeling agents can be employed to understand their fates in vivo fully.…”

Section: Biomedical Applications Of Npsmentioning

confidence: 99%

“…To promote ultrasound imaging in tracking therapeutic cells by making the injected therapeutic cells more noticeable, Chen et al employed Stöber silica contrast agents for mesenchymal stem cell labeling. This labeling can enhance the ultrasound intensity of therapeutic cells with NPs [ 49 ]. In another study, they also proposed the application of exosome-like silica NPs for labeling stem cells for regenerative medicine imaging due to their ultrasound impedance mismatch.…”

Section: Biomedical Applications Of Npsmentioning

confidence: 99%

Structural parameters of nanoparticles affecting their toxicity for biomedical applications: a review

et al. 2023

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Rapidly growing interest in using nanoparticles (NPs) for biomedical applications has increased concerns about their safety and toxicity. In comparison with bulk materials, NPs are more chemically active and toxic due to the greater surface area and small size. Understanding the NPs’ mechanism of toxicity, together with the factors influencing their behavior in biological environments, can help researchers to design NPs with reduced side effects and improved performance. After overviewing the classification and properties of NPs, this review article discusses their biomedical applications in molecular imaging and cell therapy, gene transfer, tissue engineering, targeted drug delivery, Anti-SARS-CoV-2 vaccines, cancer treatment, wound healing, and anti-bacterial applications. There are different mechanisms of toxicity of NPs, and their toxicity and behaviors depend on various factors, which are elaborated on in this article. More specifically, the mechanism of toxicity and their interactions with living components are discussed by considering the impact of different physiochemical parameters such as size, shape, structure, agglomeration state, surface charge, wettability, dose, and substance type. The toxicity of polymeric, silica-based, carbon-based, and metallic-based NPs (including plasmonic alloy NPs) have been considered separately.

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“…However, this approach has two advantages: it will be possible to have a long-term follow-up of the cells if the trackers are expressed by the cells and the cell viability could be confirmed. [110,[122][123][124][125][126][127][128][129][130][131][132][133][134][135][136][137][138][139][140][141]. SPIO: superparamagnetic iron oxides; 111lnOc: (111)In-oxine; 18F-FDG: [fluorine-18]fluoro-D-glucose; 64 Cu-PTSM: 64Cu-pyruvaldehyde-bis(N4-methylthiosemicarbazone); RFP: red fluorescent protein; GFP: green fluorescent protein; Fluc: firefly luciferase; Rluc: renilla luciferase; NIS: sodium iodide symporter; HSV1-TK: herpes simplex virus type 1-thymidine kinase; D2R: Dopamine type 2 receptor.…”

Section: Biodistributionmentioning

confidence: 99%

Development of Multilayer Mesenchymal Stem Cell Cell Sheets

Ochiai¹,

Niihara²,

Oliva³

2021

IJTM

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Cell and gene therapies have been developing dramatically over the past decade. To face and adapt to the development of these new therapies, the Food and Drug Administration (FDA) wrote and updated new guidelines from 2016 and keep updating them. Mesenchymal stem cells (MSCs) are the most used cells for treatment, far ahead from the induced pluripotent stem cells (iPSCs), based on registered clinical trials at clinicaltrials.gov. They are widely used because of their differentiation capacity and their anti-inflammatory properties, but some controversies still require clear answers. Additional studies are needed to determine the dosage, the number, and the route of injections (location and transplantation method), and if allogenic MSCs are safe compared to autologous MSC injection, including their long-term effect. In this review, we summarize the research our company is conducting with the adipose stromal cells in engineering cell sheets and their potential application.

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Stem Cell Tracking with Nanoparticle-Based Ultrasound Contrast Agents

Cited by 7 publications

References 22 publications

Magnetic Nanobubble Mechanical Stress Induces the Piezo1‐Ca²⁺‐BMP2/Smad Pathway to Modulate Neural Stem Cell Fate and MRI/Ultrasound Dual Imaging Surveillance for Ischemic Stroke

Magnetic Nanobubble Mechanical Stress Induces the Piezo1‐Ca²⁺‐BMP2/Smad Pathway to Modulate Neural Stem Cell Fate and MRI/Ultrasound Dual Imaging Surveillance for Ischemic Stroke

Structural parameters of nanoparticles affecting their toxicity for biomedical applications: a review

Development of Multilayer Mesenchymal Stem Cell Cell Sheets

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Stem Cell Tracking with Nanoparticle-Based Ultrasound Contrast Agents

Cited by 7 publications

References 22 publications

Magnetic Nanobubble Mechanical Stress Induces the Piezo1‐Ca2+‐BMP2/Smad Pathway to Modulate Neural Stem Cell Fate and MRI/Ultrasound Dual Imaging Surveillance for Ischemic Stroke

Magnetic Nanobubble Mechanical Stress Induces the Piezo1‐Ca2+‐BMP2/Smad Pathway to Modulate Neural Stem Cell Fate and MRI/Ultrasound Dual Imaging Surveillance for Ischemic Stroke

Structural parameters of nanoparticles affecting their toxicity for biomedical applications: a review

Development of Multilayer Mesenchymal Stem Cell Cell Sheets

Contact Info

Product

Resources

About

Magnetic Nanobubble Mechanical Stress Induces the Piezo1‐Ca²⁺‐BMP2/Smad Pathway to Modulate Neural Stem Cell Fate and MRI/Ultrasound Dual Imaging Surveillance for Ischemic Stroke

Magnetic Nanobubble Mechanical Stress Induces the Piezo1‐Ca²⁺‐BMP2/Smad Pathway to Modulate Neural Stem Cell Fate and MRI/Ultrasound Dual Imaging Surveillance for Ischemic Stroke