Development of Multilayer Mesenchymal Stem Cell Cell Sheets

Abstract

Cell and gene therapies have been developing dramatically over the past decade. To face and adapt to the development of these new therapies, the Food and Drug Administration (FDA) wrote and updated new guidelines from 2016 and keep updating them. Mesenchymal stem cells (MSCs) are the most used cells for treatment, far ahead from the induced pluripotent stem cells (iPSCs), based on registered clinical trials at clinicaltrials.gov. They are widely used because of their differentiation capacity and their anti-inflammatory properties, but some controversies still require clear answers. Additional studies are needed to determine the dosage, the number, and the route of injections (location and transplantation method), and if allogenic MSCs are safe compared to autologous MSC injection, including their long-term effect. In this review, we summarize the research our company is conducting with the adipose stromal cells in engineering cell sheets and their potential application.

Nanotechnology and bioengineering approaches to improve the potency of mesenchymal stem cell as an off‐the‐shelf versatile tumor delivery vehicle

“…Additionally, cell sheet technology can retain cell-cell connections and ECM. 347 Therapeutic MSC sheets produced by cell sheet technology can maintain endogenous cell matrix, adhesive proteins, and receptors, thus improving cell viability and communication and promoting sustained cellular activity by preserving natural cellular environments. The preservation of the ECM can improve the grafting of cell sheets on the target tissue and increase cell survival.…”

“…The preservation of the ECM can improve the grafting of cell sheets on the target tissue and increase cell survival. 347 Furthermore, MSC sheets produced by cell sheet technology can also be used as a bioactive drug delivery depot, simultaneously localizing and concentrating MSCs in the targeted tissue while enabling them to exert sustained secretion of regenerative cytokine and dynamic signaling in response to a disease condition. 348 MSC sheets can be used as a regenerative therapeutic agent to heal the side effects of chemotherapy and tumor resection injuries.…”

“…Second, the absence of a scaffold means there is no risk of fibrosis in the space left during the scaffold degradation. Additionally, cell sheet technology can retain cell–cell connections and ECM 347 . Therapeutic MSC sheets produced by cell sheet technology can maintain endogenous cell matrix, adhesive proteins, and receptors, thus improving cell viability and communication and promoting sustained cellular activity by preserving natural cellular environments.…”

“…Therapeutic MSC sheets produced by cell sheet technology can maintain endogenous cell matrix, adhesive proteins, and receptors, thus improving cell viability and communication and promoting sustained cellular activity by preserving natural cellular environments. The preservation of the ECM can improve the grafting of cell sheets on the target tissue and increase cell survival 347 . Furthermore, MSC sheets produced by cell sheet technology can also be used as a bioactive drug delivery depot, simultaneously localizing and concentrating MSCs in the targeted tissue while enabling them to exert sustained secretion of regenerative cytokine and dynamic signaling in response to a disease condition 348 .…”