Stem Cell Therapy for Liver Diseases

Siddiqui, Aliya; Jeevani, T; P, Naga Anusha; Bindu, Hima

doi:10.4172/2157-7633.1000111

Cited by 2 publications

(2 citation statements)

References 82 publications

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“…A multipotent cell could be further differentiated into various but limited cell types, while a pluripotent cell could be differentiated into any desired cell type ( Figure 1). This process holds great promise for biomedical applications to use it as a potential treatment for human diseases and study cellular pathology in the cell culture dish [1][2][3][4][5][6][7][8]. Studies demonstrated that differentiated cells can be reprogrammed to an embryonic-like pluripotent state [9] or a multipotent state [10][11][12] and by that generated a lot of interest in understanding the molecular basis of cell fate determination and developmental biology.…”

Section: Introductionmentioning

confidence: 99%

Exploring the Neural Reprogrammome using Bioinformatics Approaches

Thummer¹

2013

J Stem Cell Res Ther

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Recent studies demonstrate that mammalian cells can be artificially reprogrammed by ectopic expression of transcription factors in an unforeseen straightforward manner. Patient-derived reprogrammed cells hold great potential for biomedical applications such as cell replacement therapy, drug toxicity studies and disease modeling. Somatic cells such as fibroblasts can be dedifferentiated into so-called induced pluripotent stem cells (iPSCs) that are similar to embryonic stem cells (ESCs) by overexpression of Oct4, Sox2, Klf4 and cMyc. However, clinical applications using iPSCs carry the risk of tumor formation due to incomplete differentiation. More recently, it has been demonstrated that transcription factor-driven reprogramming enables direct conversion of fibroblasts into neurons, cardiomyocytes, hepatocytes as well as neural progenitors. Various groups elaborated protocols for the direct conversion of fibroblasts into multipotent induced neural stem cells (iNSCs) using different combinations of transcription factors and media conditions. These studies have shown that iNSCs exhibit morphology, gene expression and self-renewing capacity similar to NSCs derived from primary tissue. Moreover, these iNSCs differentiated into neurons, astrocytes and oligodendrocytes indicating multipotency of these cells. Here, we compare the gene expression profile of reprogrammed cells reported in these studies to determine the similarity in expression profile between the generated iNSCs using bioinformatics approaches. We provide a general workflow that can be applied to evaluate the status of reprogrammed cell populations. Using hierarchical clustering analysis and principal component analysis (PCA), we show that iNSCs reported by Thier et al. resemble more closely to those of Han et al. On the other hand, iNSCs generated by Ring et al. are relatively similar to 4F iNSC (late) of the study by Han et al. as judged by hierarchical clustering analysis. Our study demonstrates that bioinformatics approaches are particularly valuable to robustly assess the transcriptional status of reprogrammed cells and to anticipate their cellular functionality.

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Section: Introductionmentioning

confidence: 99%

Exploring the Neural Reprogrammome using Bioinformatics Approaches

Thummer¹

2013

J Stem Cell Res Ther

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“…Human stem cell research holds an enormous potential to contribute the understanding of fundamental human biology [7]. Because of their ability stem cells can differentiate into any tissue type, they have huge potential in various disease therapies and traumatic injuries [8].…”

Section: Introductionmentioning

confidence: 99%

Potency of Various Types of Stem Cells and their Transplantation

A¹,

Srilatha²

2011

J Stem Cell Res Ther

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Stem cells are stirring great excitement in medical research. Stem cells are characterized by their three special properties of self renewal, differentiation and unspecialized nature. The capacity to differentiate into specialized cell types and be able to give rise to any mature cell type is referred to as potency. Human stem cell research holds an enormous potential and contribute to the understanding of fundamental human biology. Stem cell therapy is an amazing modern medical advancement that goes straight to the source of the problem and treats various disorders. Stem cell therapies are playing a major role in cell transplantation biology. This review describes various types of stem cells, their potency and transplantation.

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Stem Cell Therapy for Liver Diseases

Cited by 2 publications

References 82 publications

Exploring the Neural Reprogrammome using Bioinformatics Approaches

Exploring the Neural Reprogrammome using Bioinformatics Approaches

Potency of Various Types of Stem Cells and their Transplantation

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