Stem cell recruitment and angiogenesis of neuropeptide substance P coupled with self-assembling peptide nanofiber in a mouse hind limb ischemia model

Self-Assembling Peptide Nanofibers Coupled with Neuropeptide Substance P for Bone Tissue Engineering

“…Numerous studies were reported that SP not only recruited MSCs to the injured site but also enhanced angiogenesis. 6,8,[12][13][14][15]17 SP-modified peptide gel (RADA16/ RADA16-SP) was applied to a mouse hind limb ischemia model in our previous work in which RADA16/RADA16-SP enhanced angiogenesis and prevented fibrosis and apoptosis in the injured site. 6 From these studies, we considered that KLD12/KLD12-SP could be a biomaterial for tissue regeneration without cell transplantation.…”

“…For CD spectra measurements, samples were added to a quartz cuvette with a 1-mm path length (Hellam Standard Cuvet 110QS (Quartz Suprasil); Hellma Analtics). 6 Preparation of the PLA/b-TCP scaffold…”

“…However, although cell transplantation results provide good prognosis, there are some obstacles such as cell source limitations, invasiveness, time requirements and the cost of expansion. 6 To overcome these limitations, many studies have tried to mimic endogenous wound-healing processes instead of using stem cell transplantation. When tissue is damaged, various signaling factors are secreted to initiate the stem cell mobilization process.…”

Engineering Integrative Stem Cell and Biomaterial Therapies for Peripheral Artery Disease

“…After two weeks post-injection, compared to empty gel controls, the peptide-loaded hydrogel displayed 1.4-fold increase in perfusion by Doppler imaging, 3-fold higher fluorescent reading in microangiography and 0.1-fold reading compared to controls in macrophage presence in the local tissue. Another group used a similar strategy by grafting substance P, a small peptide known to aid in healing by mobilizing stromal cells that helps tissue reconstruction and inflammation control, which had sustained release for nearly one month and demonstrated similar significant improvement in limb perfusion [115].…”

Applications of self-assembling peptide scaffolds in regenerative medicine: the way to the clinic

“…In 2013, Kim et al demonstrated the regeneration of functional blood vessels in the hind limbs of ischemic rats using RADA 16-II peptide scaffolds. 47 The workers delivered neuropeptide Substance P for 28 days by immobilizing it with RADA scaffolds (Fig. 7).…”