Stem Cell Properties of Human Dental Pulp Stem Cells

Abstract

In this study, we characterized the self-renewal capability, multi-lineage differentiation capacity, and clonogenic efficiency of human dental pulp stem cells (DPSCs). DPSCs were capable of forming ectopic dentin and associated pulp tissue in vivo. Stromal-like cells were reestablished in culture from primary DPSC transplants and re-transplanted into immunocompromised mice to generate a dentin-pulp-like tissue, demonstrating their self-renewal capability. DPSCs were also found to be capable of differentiating into adipocytes and neural-like cells. The odontogenic potential of 12 individual single-colony-derived DPSC strains was determined. Two-thirds of the single-colony-derived DPSC strains generated abundant ectopic dentin in vivo, while only a limited amount of dentin was detected in the remaining one-third. These results indicate that single-colony-derived DPSC strains differ from each other with respect to their rate of odontogenesis. Taken together, these results demonstrate that DPSCs possess stem-cell-like qualities, including self-renewal capability and multi-lineage differentiation.

The static magnetic field accelerates the osteogenic differentiation and mineralization of dental pulp cells

“…They contain progenitor/stem cells that can differentiate into dentin-forming odontoblasts and maintain the homeostasis of dental mineralized tissue by polarization and secretion of predentindentin components, in response to the appropriate stimuli, e.g. caries, chemicals or trauma (Tonomura et al 2007;Gronthos et al 2002). Researches indicated that DPCs whether from human, pigs or rats were recognized as a source of mesenchymal stem cells (Yang et al 2007;Zhang et al 2005;Alliot-Licht et al 2005).…”

The Performance of Human Periodontal Ligament Mesenchymal Stem Cells on Xenogenic Biomaterials

“…No tooth extraction was needed and explants, derived from the alveolar crestal and horizontal fibers of PDL, allowed the migration of a population ofMSCs, all showing the same morphological and phenotypical features previously described (25)(26). The evaluation under contrast phase light microscopy of ex-vivo expanded PDL-MSCs cultured with osteogenic medium after 3 and 4 weeks showed the…”

Dental Kök Hücre Kaynaklari Ve Kemi̇k Doku Rejenerasyonunda Kullanilma Potansi̇yelleri̇

“…49 Bir farenin immun sistemi baskılanarak sırtına transplante edilen dental pulpa kök hücrelerinin dentin-pulpa kompleksine benzer bir yapı oluşturduğu izlenmiştir. [50][51][52][53] Düşen süt dişleri, kök hücre çalışmalarında etik açıdan bir sorun olmadan kullanılabilmektedir-ler. 37,38,43,44,[54][55][56] Bu hücrelerin osteoblastlar, odontoblastlar, adipositler, kondrositler, ve hepatositler gibi çeşitli hücre tiplerine (in vitro ve in vivo) diferansiye olma yeteneğine sahip olduğu gösterilmiştir.…”

“…12 Yetişkinlerde kök hücre sayısı azaldığı için neredeyse kök hücrelere hiç rastlanmaz. 23,24 Embriyonik kök hücrelerin farklılaşma yeteneğinin fazla olması, bu hücreleri daha değerli kılmaktadır. 18 Ancak embriyonik kök hücrelerin elde edilmesindeki etik ve yasal tartışmalar ve tümör oluşma riski vardır.…”