Stem cell culture on polyvinyl alcohol hydrogels having different elasticity and immobilized with ECM-derived oligopeptides

Muduli, Saradaprasan; Chen, Li Hua; Li, Meng Pei; Heish, Zhao Wen; Liu, Chenghui; Kumar, Suresh; Alarfaj, Abdullah A.; Munusamy, Murugan A.; Benelli, Giovanni; Murugan, Kadarkarai; Wang, Han Chow; Chen, Da Chung; Hsu, Shih Tien; Chang, Shih Chang; Higuchi, Akon

doi:10.1515/polyeng-2016-0193

Cited by 18 publications

(30 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, active components are usually introduced into PVA hydrogels through a physical or chemical process to facilitate stem cell growth and differentiation. Such active components include hyaluronic acid [13], chitosan [16], gelatin [17], oligopeptides [18], and karaya gum [19]. For example, when preparing PVA/chitosan composite hydrogels, increasing the proportion of PVA from 80 wt% to 90 wt% changes the stem cell shape from polygonal to spherical [16].…”

Section: Discussionmentioning

confidence: 99%

Structure and properties of PVA/silk fibroin hydrogels and their effects on growth behavior of various cell types

Hou

Wang

Han

et al. 2020

Mater. Res. Express

View full text Add to dashboard Cite

Controllable regulation of cell behavior is one of the most important factors conducive to the restoration of tissue functions. Recently, various strategies have been developed using physical or chemical cues. Although these techniques are effective, the high cost and complex fabrication procedures impede their application. In this study, we used a low cost and simple strategy to fabricate PVA/silk fibroin composite hydrogels using a cyclic freeze-thaw method. With the increase of freezethaw cycles, the pore size of hydrogels decreased, the elastic modulus increased, and the swelling rate decreased. Furthermore, we chose two shapes of model cells, a spindle using bone marrow mesenchymal stem cells and smooth muscle cells, and a round shape using BV2 microglial cells. PVA/ silk fibroin composite hydrogels inhibited the adhesion and proliferation of stem cells and muscle cells and changed their cell shape from spindle to round, maintained the initial round shape of BV2 microglial cells, and promoted the proliferation of BV2 microglial cells. These results demonstrate that PVA/silk fibroin composite hydrogels can be used as a novel hydrogel system to regulate cell behavior.

show abstract

Section: Discussionmentioning

confidence: 99%

Structure and properties of PVA/silk fibroin hydrogels and their effects on growth behavior of various cell types

Hou

Wang

Han

et al. 2020

Mater. Res. Express

View full text Add to dashboard Cite

show abstract

“…Hydrogels, which may be characterized by their hydrophilicity, have been considered potential scaffolds for stem cell culture mostly due to its 3D structure that have been used to enhance cell viability and adhesion (Drozdova et al, ; Gu et al, ; Gupta, Greeshma, Basu, Goswami, & Sinha, ; Kim & Kim, ; Li, Sun, Li, Kawazoe, & Chen, ; Mori & Hara, ; Muduli et al, ; Tsou, Khoneisser, Huang, & Xu, ; Zhang et al, ; Zhu & Marchant, ). Structurally, they may be composed of macro, micro, or nanopores, which enable cell mobility and differentiation (Higuchi, Ling, Chang, Hsu, & Umezawa, ; Kretlow & Mikos, ; Shoichet, ).…”

Section: Introductionmentioning

confidence: 99%

“…Particularly, poly(vinyl alcohol) (PVA) is a promising material as a scaffold for tissue engineering as it is nontoxic (Gupta, Webster, & Sinha, ; Liu, Vrana, Cahill, & McGuinness, ; Teodorescu, Bercea, & Morariu, ). Moreover, its mechanical properties can be tuned to obtain hydrogels for specific needs of each cell type (Drozdova et al, ; Gupta et al, ; Muduli et al, ). PVA hydrogels have been chemically modified to create a suitable environment for fibroblasts (Drozdova et al, ; Muduli et al, ), neural stem cells (Mori & Hara, ), and myoblasts (Gupta et al, ), primarily representing an alternative biomaterial for culture of iPS (Muduli et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, its mechanical properties can be tuned to obtain hydrogels for specific needs of each cell type (Drozdova et al, 2017;Gupta et al, 2013;Muduli et al, 2017). PVA hydrogels have been chemically modified to create a suitable environment for fibroblasts (Drozdova et al, 2017;Muduli et al, 2017), neural stem cells (Mori & Hara, 2016), and myoblasts (Gupta et al, 2013), primarily representing an alternative biomaterial for culture of iPS (Muduli et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Interaction of fibroblasts and induced pluripotent stem cells with poly(vinyl alcohol)‐based hydrogel substrates

et al. 2019

View full text Add to dashboard Cite

Induced pluripotent stem cells (iPSCs) provide a promising means of creating custom‐tailored cell lines for cellular therapies. Their application in regenerative medicine, however, depends on the possibility that the maintenance and differentiation of cells and organs occur under defined conditions. One major component of stem cell culture systems is the substrate, where the cells must attach and proliferate. The present study aimed to investigate the putative cytotoxic effects of poly(vinyl alcohol) (PVA)‐based matrices on the in vitro culture of mouse fetal fibroblasts. In addition, the PVA‐based hydrogels were used to determine the capacity of bovine induced pluripotent stem cells (biPSCs) to adhere and proliferate on synthetic substrates. Our results show that both cell types interacted with the substrate and presented proliferation during culture. The biPSCs formed new colonies when cell suspensions were placed onto the hydrogel surface for culture. These results may represent a new characterized xeno‐free clinical grade culture system to be widely applied in cell‐based therapies.

show abstract

“…Therefore, it is important to develop another type of hydrogel, of which the stiffness can be controlled by crosslinking of the hydrogels. For this purpose, bioinert hydrogels were developed, which were prepared from polyvinyl alcohol-co-itaconic acid (P-IA) with a different stiffness, which was controlled by the crosslinking degree with a changing crosslinking time 25,26,27,28,29,30,31,32 . The stem cells can be cultivated on nonmodified P-IA hydrogels, as well as P-IA hydrogels grafted with extracellular matrices (ECMs) and oligopeptides.…”

Section: Introductionmentioning

confidence: 99%

Human Pluripotent Stem Cell Culture on Polyvinyl Alcohol-Co-Itaconic Acid Hydrogels with Varying Stiffness Under Xeno-Free Conditions

Sung

Higuchi

et al. 2018

JoVE

Self Cite

View full text Add to dashboard Cite

The effect of physical cues, such as the stiffness of biomaterials on the proliferation and differentiation of stem cells, has been investigated by several researchers. However, most of these investigators have used polyacrylamide hydrogels for stem cell culture in their studies. Therefore, their results are controversial because those results might originate from the specific characteristics of the polyacrylamide and not from the physical cue (stiffness) of the biomaterials. Here, we describe a protocol for preparing hydrogels, which are not based on polyacrylamide, where various stem, cells including human embryonic stem (ES) cells and human induced pluripotent stem (iPS) cells, can be cultured. Hydrogels with varying stiffness were prepared from bioinert polyvinyl alcohol-co-itaconic acid (P-IA), with stiffness controlled by crosslinking degree by changing crosslinking time. The P-IA hydrogels grafted with and without oligopeptides derived from extracellular matrix were investigated as a future platform for stem cell culture and differentiation. The culture and passage of amniotic fluid stem cells, adipose-derived stem cells, human ES cells, and human iPS cells is described in detail here. The oligopeptide P-IA hydrogels showed superior performances, which were induced by their stiffness properties. This protocol reports the synthesis of the biomaterial, their surface manipulation, along with controlling the stiffness properties and finally, their impact on stem cell fate using xeno-free culture conditions. Based on recent studies, such modified substrates can act as future platforms to support and direct the fate of various stem cells line to different linkages; and further, regenerate and restore the functions of the lost organ or tissue.

show abstract

Stem cell culture on polyvinyl alcohol hydrogels having different elasticity and immobilized with ECM-derived oligopeptides

Cited by 18 publications

References 56 publications

Structure and properties of PVA/silk fibroin hydrogels and their effects on growth behavior of various cell types

Structure and properties of PVA/silk fibroin hydrogels and their effects on growth behavior of various cell types

Interaction of fibroblasts and induced pluripotent stem cells with poly(vinyl alcohol)‐based hydrogel substrates

Human Pluripotent Stem Cell Culture on Polyvinyl Alcohol-Co-Itaconic Acid Hydrogels with Varying Stiffness Under Xeno-Free Conditions

Contact Info

Product

Resources

About