Stem cell-compatible eumelanin biointerface fabricated by chemically controlled solid state polymerization

Pezzella, Alessandro; Barra, Mario; Musto, Anna; Navarra, Angelica; Alfè, Michela; Manini, Paola; Parisi, Silvia; Cassinese, A.; Criscuolo, Valeria; d’Ischia, Marco

doi:10.1039/c4mh00097h

Cited by 98 publications

(115 citation statements)

References 49 publications

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“…These results showed that melanin thin films have great potential in the reconstruction of tissues, being biodegradable, and possess inflammatory response comparable to silicone. Another study of the biocompatibility of melanin thin films demonstrated that the melanin film effectively supports the growth of undifferentiated stem cells and their differentiation into neuronal precursors and neurons [146]. They related that high-quality melanin thin films display appealing features, such as reversible conductivity by controlled hydration-dehydration steps-excellent biocompatibility with stem cells, and water-resistant adhesion, for bioelectronic applications, e.g., in organic electrochemical transistors (OECTs), which can translate cellular activity into electrical signals [125,147].…”

Section: Medical Applicationsmentioning

confidence: 99%

Production of Melanin Pigment by Fungi and Its Biotechnological Applications

Sponchiado¹,

Sousa²,

Andrade³

et al. 2017

Melanin

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Production of the microbial pigments is one of the emerging fields of research due to a growing interest of the industry for safer products, easily degradable and eco-friendly. Fungi constitute a valuable source of pigments because they are capable of producing high yields of the substance in the cheap culture medium, making the bioprocess economically viable on the industrial scale. Some fungal species produce a dark-brown pigment, known as melanin, by oxidative polymerization of phenolic compounds, such as glutaminyl-3,4-dihydroxybenzene (GDHB) or catechol or 1,8-dihydroxynaphthalene (DHN) or 3,4-dihydroxyphenylalanine (DOPA). This pigment has been reported to act as "fungal armor" due to its ability to protect fungi from adverse conditions, neutralizing oxidants generated in response to stress. Apart from the scavenging activity, melanin exhibits other biological activities, including thermoregulatory, radio-and photoprotective, antimicrobial, antiviral, cytotoxic, anti-inflammatory, and immunomodulatory. Studies have shown that the media composition and cultivation conditions affect the pigment production in fungi and the manipulation of these parameters can result in an increase in pigment yield for large-scale pigment production. This chapter presents a comprehensive discussion of the research on fungal melanin, including the recently discovered biological activities and the potential use of this pigment for various biotechnological applications in the fields of biomedicine, dermocosmetics, materials science, and nanotechnology.

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Section: Medical Applicationsmentioning

confidence: 99%

Production of Melanin Pigment by Fungi and Its Biotechnological Applications

Sponchiado¹,

Sousa²,

Andrade³

et al. 2017

Melanin

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“…An alternative route for eumelanin coating has been proposed recently, 41 which allows to overcome the main issues associated with the limited processability of eumelanins due to insolubility in most solvents. Typically, eumelanin thin films are obtained by direct deposition of the pigment from aqueous alkali favoring partial solubilization due to oxidative backbone modification.…”

Section: −10mentioning

confidence: 99%

“…The subsequent freezedrying at −60°C for 2 days afforded white cylindrical TOCNFaerogels with a final volume of 7 cm 3 , a bulk density d b of about 40 mg cm −3 , and a resulting porosity of 97.5%. TOCNFaerogels thus prepared were coated with eumelanin using the recently developed AISSP 41 of DHI (Scheme 2) and DHICA. Figure 2 shows complete aerogel coating, as apparent from the dark colorations, black in the case of DHI, or dark brown in the case of DHICA.…”

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confidence: 99%

Surface-Functionalization of Nanostructured Cellulose Aerogels by Solid State Eumelanin Coating

et al. 2016

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Bioinspired aerogel functionalization by surface modification and coating is in high demand for biomedical and technological applications. In this paper, we report an expedient three-step entry to all-natural surface-functionalized nanostructured aerogels based on (a) TEMPO/NaClO promoted synthesis of cellulose nanofibers (TOCNF); (b) freezedrying for aerogel preparation; and (c) surface coating with a eumelanin thin film by ammonia-induced solid state polymerization (AISSP) of 5,6-dihydroxyindole (DHI) or 5,6-dihydroxyindole-2-carboxylic acid (DHICA) previously deposited from an organic solution. Scanning electron microscopy showed uniform deposition of the dark eumelanin coating on the template surface without affecting porosity, whereas solid state 13 C NMR and electron paramagnetic resonance (EPR) spectroscopy confirmed the eumelanin-type character of the coatings. DHI melanin coating was found to confer to TOCNF templates a potent antioxidant activity, as tested by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays as well as strong dye adsorption capacity, as tested on methylene blue. The unprecedented combination of nanostructured cellulose and eumelanin thin films disclosed herein implements an original all-natural multifunctional aerogel biomaterial realized via an innovative coating methodology.

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“…15 Since the seminal work of McGinness 16 on electrical transport in eumelanin in 1974, many have endeavored to exploit eumelanin as a natural active material for bioelectronics. 10,[17][18][19] Understanding the nature of eumelanin conductivity is complicated by its heterogeneous nature, and two models for its structure exist: the extended…”

Section: Introductionmentioning

confidence: 99%

Aqueous photo(electro)catalysis with eumelanin thin films

et al. 2018

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We report that eumelanin, the ubiquitous natural pigment found in most living organisms, is a photocatalytic material. Though the photoconductivity of eumelanin and its photochemical reactions with oxygen have been known for some time, eumelanins have not been regarded as photofaradaic materials. We find that eumelanin shows photocathodic behavior for both the oxygen reduction reaction and the hydrogen evolution reaction. Eumelanin films irradiated in aqueous solutions at pH 2 or 7 with simulated solar light photochemically reduce oxygen to hydrogen peroxide with accompanying oxidation of sacrificial oxalate, formate, or phenol.

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Stem cell-compatible eumelanin biointerface fabricated by chemically controlled solid state polymerization

Abstract: A high quality eumelanin thin film featuring efficient reversibility of the water induced conductivity switch and high biocompatibility was obtained,viaammonia-induced solid state polymerization of a 5,6-dihydroxyindole thin film.

Cited by 98 publications

References 49 publications

Production of Melanin Pigment by Fungi and Its Biotechnological Applications

Production of Melanin Pigment by Fungi and Its Biotechnological Applications

Surface-Functionalization of Nanostructured Cellulose Aerogels by Solid State Eumelanin Coating

Aqueous photo(electro)catalysis with eumelanin thin films

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