Stearidonic acid, a plant-based dietary fatty acid, enhances the chemosensitivity of canine lymphoid tumor cells

Pondugula, Satyanarayana R.; Ferniany, Glennie; Ashraf, Farah; Abbott, Kodye L.; Smith, B. F.; Coleman, Elaine S.; Mansour, Mahmoud; Bird, Richard M.; Smith, Annette; Karthikeyan, Chandrabose; Trivedi, Piyush; Tiwari, Amit K.

doi:10.1016/j.bbrc.2015.03.141

Cited by 14 publications

(17 citation statements)

References 33 publications

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“…Of particular note in relation to veterinary medicine and faty acids are two studies on canine tumor cell lines. The irst is that of canine lymphoma cell lines; in this study, stearidonic acid was shown to sensitize cells to anticancer drugs, even when the cells were previously resistant to drugs [84]. The second study utilized faty acids themselves as antitumor agents.…”

Section: Cancer Associations With Faty Acidsmentioning

confidence: 95%

Fatty Acids in Veterinary Medicine and Research

Simpson¹,

Mostyn²

2017

Fatty Acids

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Faty acid regulation is an essential process for all animals. A number of studies have shown that diet afects the levels/availability of faty acids in the body but increasingly an evidence shows that disease states can alter the amounts within the body too. Faty acid levels and availability have been altered by a number of diseases, disorders and reactions including inlammatory responses, heart disease and heart failure and wound repair. They are also essential during the growth and development stages of animals. The amount of research into the consequences of diferent faty acid intake and levels in various disease states and during development has increased in both humans and animals. This review presents an overview of the research undertaken to date and highlights the importance, uses and beneits of understanding the roles of faty acids in both the healthy animals and animals under difering disorders and diseases.

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Section: Cancer Associations With Faty Acidsmentioning

confidence: 95%

Fatty Acids in Veterinary Medicine and Research

Simpson¹,

Mostyn²

2017

Fatty Acids

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“…Previous studies reported that the n-3 PUFA, such as DHA and SDA, were not cytotoxic at concentrations up to 200 μM in various cell culture systems [12,18]. In addition, it was reported that 400 μM of SDA was non-cytotoxic to healthy dog peripheral blood mononuclear cells [19]. Similar dietary fatty acids such as EPA and DHA in humans could attain up to 500 uM in plasma after oral intake in diet [20,21].…”

Section: Effects Of Sda On Cell Viability and Cytotoxicity In Lps-stimentioning

confidence: 98%

Anti‐Inflammatory Effects of Stearidonic Acid Mediated by Suppression of NF‐κB and MAP‐Kinase Pathways in Macrophages

Sung

Jeon²,

Kim

et al. 2017

Lipids

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Stearidonic acid (SDA, 18:4n-3) is an omega-3 polyunsaturated fatty acid present in oils derived from plants of the Boraginaceae family. In this study, we determined the anti-inflammatory effects of SDA isolated from echium oil on lipopolysaccharide (LPS)-induced inflammatory responses in RAW 264.7 macrophages. SDA significantly downregulated the levels of the inducible nitric oxide synthase (iNOS) protein, thereby suppressing the production of nitric oxide (NO) in LPS-stimulated RAW 264.7 cells. In addition, SDA inhibited the nuclear translocation and promoter activity of nuclear factor κB (NFκB) and the phosphorylation of mitogen-activated protein kinases (MAPK) such as extracellular signal regulated kinase 1/2, c-jun N terminal kinase, and p38 in LPS-stimulated RAW 264.7 cells. Our results showed that SDA exerted anti-inflammatory effects by suppressing iNOS-mediated NO production via inactivation of NFκB and MAPK signaling pathways.

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“…The measurements were performed in the presence or absence of a known ABCG2 inhibitor, nilotinib, or IND compounds. The ratio of intracellular Rh concentrations in the absence and presence of 5 μM nilotinib or IND compounds is indicative of the efflux activity of ABCG2 [17,23]. As shown in Fig.…”

Section: The Effect Of Ind Compounds and Nilotinib On The Intracellulmentioning

confidence: 99%

“…Rh (5 μM) was added to the cells in the presence or absence of nilotinib (positive control) or IND compounds and incubated for another 1 h. Subsequently, the medium was aspirated and the cells were washed with ice-cold PBS, trypsinized, washed three times with ice-cold PBS, and solubilized in 0.1% triton-PBS. To determine the intracellular concentration of Rh, the fluorescence was measured using Infinite microplate reader (Tecan Trading AG, Switzerland) at an excitation wavelength of 485 nm and an emission wavelength of 538 nm [17,23]. The ratio of intracellular Rh concentrations in the absence and presence of nilotinib or IND compounds will indicate the efflux activity of ABCG2.…”

Section: Intracellular Rhodamine 123 Accumulation Assaymentioning

confidence: 99%

“…The efflux activity of ABCG2 was determined by measuring the intracellular accumulation of the fluorescent probe rhodamine 123 (Rh) [17,23]. Briefly, confluent H460-MX20 cells in 6-well plates were preincubated with or without nilotinib (positive control), IND-1 or IND-3 to IND-8 for 1 h in phenol red-free DMEM at 37°C.…”

Section: Intracellular Rhodamine 123 Accumulation Assaymentioning

confidence: 99%

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