Steaming combined with biochar application eliminates negative plant-soil feedback for sanqi cultivation

Yang, Min; Yuan, Y.; Huang, Huichuan; Wang, Hongzhi; Guo, Changhong; Xu, Yanguo; Wang, Wenpeng; He, Xiahong; Liu, Yixiang; Zhu, Shusheng

doi:10.1016/j.still.2019.02.006

Cited by 41 publications

(53 citation statements)

References 42 publications

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“…The effect of sanqi growth on soil bacteria and fungi was assessed on plates according to Yang et al’s (2019) method. Briefly, 10 g of each soil sample was added to 90 mL of a 0.1% (w/v) solution of sodium pyrophosphate.…”

Section: Methodsmentioning

confidence: 99%

Negative Plant-Soil Feedback Driven by Re-assemblage of the Rhizosphere Microbiome With the Growth of Panax notoginseng

et al. 2019

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There is a concerted understanding of the accumulation of soil pathogens as the major driving factor of negative plant-soil feedback (NPSF). However, our knowledge of the connection between plant growth, pathogen build-up and soil microbiome assemblage is limited. In this study, significant negative feedback between the soil and sanqi ( Panax notoginseng ) was found, which were caused by the build-up of the soil-borne pathogens Fusarium oxysporum , F. solani , and Monographella cucumerina . Soil microbiome analysis revealed that the rhizospheric fungal and bacterial communities were changed with the growth of sanqi. Deep analysis of the phylum and genus levels corroborated that rhizospheric fungal Ascomycota, including the soil-borne pathogens F. oxysporum , F. solani , and especially M. cucumerina , were significantly enriched with the growth of sanqi. However, the bacteria Firmicutes and Acidobacteria, including the genera Pseudomonas , Bacillus, Acinetobacter and Burkholderia , were significantly suppressed with the growth of sanqi. Using microbial isolation and in vitro dual culture tests, we found that most isolates derived from the suppressed bacterial genera showed strong antagonistic ability against the growth of sanqi soil-borne pathogens. Interestingly, inoculation of these suppressed isolates in consecutively cultivated soil could significantly alleviate NPSF. In summary, sanqi growth can suppress antagonistic bacteria through re-assemblage of the rhizosphere microbiome and cause the accumulation of soil-borne pathogens, eventually building negative feedback loops between the soil and plants.

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Section: Methodsmentioning

confidence: 99%

Negative Plant-Soil Feedback Driven by Re-assemblage of the Rhizosphere Microbiome With the Growth of Panax notoginseng

et al. 2019

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“…It grows at an altitude of 400-800 m above sea level under the shades of forests and at the slopes of mountains. It has several vital functions, including being hemostatic, blood stasis dissipating, and discutient, exhibiting acesodyne effects and antihypertensive, antithrombotic, anti-atherosclerotic, and neuroprotective actions (Yang et al, 2010(Yang et al, , 2019He et al, 2012;Guo et al, 2020). With the over-exploitation and predatory activities of wild ginseng, the available resources have become scarce.…”

Section: Introductionmentioning

confidence: 99%

“…Replanting of P. notoginseng has also failed due to low germination, poor seedling growth, and high seedling death rates (Yang et al, 2015). Soil-borne disease, nutrients deficiency, auto-toxicity, and the retrograde soil physicochemical properties are the factors that affect the replanting of P. notoginseng seedlings (Yang et al, 2019). Root-rot caused by the pathogen Fusarium oxysporum is a severe disease that inhibits the replantation of P. notoginseng plants (Dong et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Probiotic Consortia: Reshaping the Rhizospheric Microbiome and Its Role in Suppressing Root-Rot Disease of Panax notoginseng

et al. 2020

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“…Bacteria and fungi were isolated on plates according to Yang's method (Yang et al 2019). Briefly, 10 g of each soil sample was added to 90 mL of a 0.1% (w/v) solution of sodium pyrophosphate.…”

Section: Isolation and Identification Of Culturable Fungi And Bacterimentioning

confidence: 99%

Ginsenosides in root exudates of Panax notoginseng drive the change of soil microbiota through carbon source different utilization

et al. 2020

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Background and aims Ginsenosides are the main bioactive components of Panax plants which could be secreted by root and show autotoxicity to root cells or promote the growth of soil-borne pathogens. However, comprehensive understanding of the effect of ginsenosides on soil microbiota is still lacking. Methods The ginsenosides in root exudates of P. notoginseng were quantified and exogenous ginsenosides on soil microbiota were tested using 16S rRNA and ITS gene tag sequencing. Then its underlying mechanism was deciphered through studying effects of ginsenosides on growth of the ginsenoside-modified culturable fungi and bacteria as well as the relationships between these fungi and bacteria. Results Exogenous root exudates and mixtures of Rg 1 + Rb 1 + Rd had similar ability to drive the change of soil microbiota. Further studies demonstrated that Rg 1 + Rb 1 + Rd mixture could enrich or suppress special fungi and bacteria to modify soil community through differential utilization of carbon source during the early stage (30 days), followed by antagonism between ginsenoside-modified fungi and bacteria to determine soil microbial community modification at later stage (60 and 90 days). Conclusions Ginsenosides were the main substances in exogenous root exudates of P. notoginseng that drove the change in soil microbiota, mediating the special interaction between the plant and the microbiota.

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Steaming combined with biochar application eliminates negative plant-soil feedback for sanqi cultivation

Cited by 41 publications

References 42 publications

Negative Plant-Soil Feedback Driven by Re-assemblage of the Rhizosphere Microbiome With the Growth of Panax notoginseng

Negative Plant-Soil Feedback Driven by Re-assemblage of the Rhizosphere Microbiome With the Growth of Panax notoginseng

Probiotic Consortia: Reshaping the Rhizospheric Microbiome and Its Role in Suppressing Root-Rot Disease of Panax notoginseng

Ginsenosides in root exudates of Panax notoginseng drive the change of soil microbiota through carbon source different utilization

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