Steady state fermentation by yeast in a growth medium

Corresponding changes in kynurenine hydroxylase activity, membrane fluidity, and sterol composition in Saccharomyces cerevisiae mitochondria

“…Mitochondria were isolated and labeled with the fluorescent probe DPH as described in the text. Anisotropy was measured over the range of 15 3d a transition at 23.3°C as well as reduced…”

“…Growth of the organism requires sterol. Under anaerobic conditions, sterol cannot be synthesized (24), and the organism is dependent on the addition of sterol to the medium (3,15). Sterols other than ergosterol may satisfy this requirement (31).…”

An All-Glass Apparatus for the Continuous Cultivation of Yeast Under Anaerobic Conditions

“…Table 1 shows the uniformity of successive yeast crops and the dry weight per unit of cell population typically obtained with most lots of Difco yeast extract. The advantage of this culture apparatus over the one described by Brockmann and Stier (1947a) lies not only in the greater stability of the yeast population but also in the ease of operation and in the greater uniformity in the behavior of fermentations inoculated with culture yeast from this apparatus.…”

The Use of Sodium Azide for Determining the Fermentative Ability of Yeast Developed Under Different Oxygen Tensions

“…Inoculum of the type designated as C02-sparged was maintained for more than three transfers in a medium continuously sparged with tank CO2. The apparatus and operating procedure for the preparation of this type of inoculum have been described by Brockmann and Stier (1947a). Apparatus and operating procedures were directed toward the exclusion of air from the culture.…”

“…Management of fermentation. All fermentations were conducted in 1-liter allglass fermentation flasks (Brockmann and Stier, 1947a), which were held under continuous agitation in a 30 C water bath. Before inoculation the fermentation flask which contained 500 to 550 ml of medium was sparged with tank C02, rate ca.…”