Steady-state and fluorescence lifetime spectroscopy for identification and classification of bacterial pathogens

Awad, Fathi; Ramprasath, Chandrasekaran; Mathivanan, N.; Aruna, Prakasarao; Ganesan, Singaravelu

doi:10.3233/bsi-140095

Cited by 8 publications

(2 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Many researchers have investigated bacterial identification techniques without addressing the issues regarding isolating and concentrating bacteria first. Examples of bacterial identification or discrimination methods include the use of light‐based spectroscopy techniques such as fluorescence , laser‐induced breakdown spectroscopy , FTIR spectroscopy , and Raman spectroscopy . Although each of these methods can accurately identify bacteria in a matter of minutes, the methods require a pure sample for analysis.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous isolation and label‐free identification of bacteria using contactless dielectrophoresis and Raman spectroscopy

et al. 2019

View full text Add to dashboard Cite

The traditional bacterial identification method of growing colonies on agar plates can take several days to weeks to complete depending on the growth rate of the bacteria. Successfully decreasing this analysis time requires cell isolation followed by identification. One way to decrease analysis time is by combining dielectrophoresis (DEP), a common technique used for cell sorting and isolation, and Raman spectroscopy for cell identification. DEP‐Raman devices have been used for bacterial analysis, however, these devices have a number of drawbacks including sample heating, cell‐to‐electrode proximity that limits throughput and separation efficiency, electrode fouling, or inability to address sample debris. Presented here is a contactless DEP‐Raman device to simultaneously isolate and identify particles from a mixed sample while avoiding common drawbacks associated with other DEP designs. Using the device, a mixed sample of bacteria and 3 μm polystyrene spheres were isolated from each other and a Raman spectrum of the trapped bacteria was acquired, indicating the potential for cDEP‐Raman devices to decrease the analysis time of bacteria.

show abstract

Section: Introductionmentioning

confidence: 99%

Simultaneous isolation and label‐free identification of bacteria using contactless dielectrophoresis and Raman spectroscopy

et al. 2019

View full text Add to dashboard Cite

show abstract

“…To further enhance the specificity in the presented setup we simultaneously acquire temporally resolved LIF signals for each excitation pulse using fast electronics. Since the fluorescence temporal behavior depends on the involved constituents and especially their microenvironments it can vary even between substances with similar chemical composition [12,14,15]. As we show below this variation provides enough diversity for a discrimination of three chosen bacterial suspensions excited and measured at a standoff distance of 3.5 m with an accuracy of 98 %.…”

Section: Introductionmentioning

confidence: 92%

Novel standoff detection system for the classification of chemical and biological hazardous substances combining temporal and spectral laser-induced fluorescence techniques⋆

et al. 2018

View full text Add to dashboard Cite

In an effort to reduce the potential risk of human exposure to chemical and biological hazardous materials, the demand increases for a detection system which rapidly identifies possible threats from a distance to avoid direct human contact to these materials. In this scope, we present a novel detection system which is able to measure simultaneously spectrally and temporally resolved laser induced fluorescence (LIF) signals excited by two consecutive laser pulses with different central wavelengths at 266 nm and 355 nm. As shown in this paper, the setup enables fast data acquisition that provides a complete dataset in less than a few milliseconds at repetition rates of 100 Hz. Furthermore, with its modular design it can be transported easily for operation at different locations. First measurements indicate a high performance with an accuracy of more than 97% in the distinguishability of bacterial specimen within a limited set of three representative bacterial species, namely Bacillus thuringiensis, Micrococcus luteus and Oligella urethralis. Together with the consecutive classification procedure, the setup promises to become a valuable tool for standoff detection of bio-hazards.

show abstract

Quantitative Spectroscopic Characterization of Near-UV/visible E. coli (pYAC4), B. subtilis (PY79), and Green Bread Mold Fungus Fluorescence for Diagnostic Applications

Herzog

Sick

2023

J Fluoresc

View full text Add to dashboard Cite

Steady-state and fluorescence lifetime spectroscopy for identification and classification of bacterial pathogens

Cited by 8 publications

References 32 publications

Simultaneous isolation and label‐free identification of bacteria using contactless dielectrophoresis and Raman spectroscopy

Simultaneous isolation and label‐free identification of bacteria using contactless dielectrophoresis and Raman spectroscopy

Novel standoff detection system for the classification of chemical and biological hazardous substances combining temporal and spectral laser-induced fluorescence techniques⋆

Quantitative Spectroscopic Characterization of Near-UV/visible E. coli (pYAC4), B. subtilis (PY79), and Green Bread Mold Fungus Fluorescence for Diagnostic Applications

Contact Info

Product

Resources

About