Status of the crystallography beamlines at PETRA III

Abstract

Since 2013, three beamlines for macromolecular crystallography are available to users at the thirdgeneration synchrotron PETRA III in Hamburg: P11, P13 and P14, the latter two operated by EMBL. Beamline P11 is operated by DESY and is equipped with a Pilatus 6M detector. Together with the photon flux of 2 × 10 13 ph/s provided by the very brilliant X-ray source of PETRA III, a full data set can be typically collected in less than 2 min. P11 provides state-of-the-art microfocusing capabilities with beam sizes down to 1 × 1 µm 2 , which makes the beamline ideally suited for investigation of microcrystals and serial crystallography experiments. An automatic sample changer allows fast sample exchange in less than 20 s, which enables high-throughput crystallography and fast crystal screening. For sample preparation, an S2 biosafety laboratory is available in close proximity to the beamline.

Dynamic Complex‐to‐Complex Transformations of Heterobimetallic Systems Influence the Cage Structure or Spin State of Iron(II) Ions

“…Very slow evaporation of solvent from an acetonitrile solution of CU‐1 (BF 4 ) 28 over 3 months yielded single crystals, which diffracted up to 1.2 Å using synchrotron radiation and allowed for unambiguous structure determination (Figure ).…”

Donor‐Site‐Directed Rational Assembly of Heteroleptic cis‐[Pd2L2L′2] Coordination Cages from Picolyl Ligands

“…We thank Dr. Andreas Brockmeyer and Dr. Petra Janning (Max‐Planck Institute for Molecular Physiology, Dortmund) and Dr. Holm Frauendorf (Georg‐August University Göttingen) for mass spectra measurements. Diffraction data for [(BF 4 ) 2.4 Cl 0.6 @Pd 4 P o 8 ] 5+ was collected at PETRA III at DESY, a member of the Helmholtz Association (HGF). The authors thank Dr. Anja Burkhardt for assistance in using synchrotron beamline P11 (I‐20160736).…”

The crystal structure of heme d1 biosynthesis-associated small c-type cytochrome NirC reveals mixed oligomeric states in crystallo

“…Data were collected at beamline P11 of the PETRAIII synchrotron (DESY, Hamburg, Germany) (Burkhardt et al, 2016). The anomalous data set was processed using DIALS (Winter et al, 2018) , POINTLESS (Evans, 2011) and AIMLESS (Evans & Murshudov, 2013) of the CCP4 suite .…”