Status epilepticus induced by pentylenetetrazole increases short-term synaptic facilitation in the hippocampus of juvenile rats

Postnikova, Tatyana Y.; Trofimova, Alina M.; Zaitsev, Aleksey V.; Magazanik, L. G.

doi:10.1134/s0012496617060102

Cited by 7 publications

(2 citation statements)

References 10 publications

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“…In the present study, seizures were induced by i.p. administration of a subconvulsive dose of PTZ, which is a GABA-A receptor antagonist, at 48 h intervals for 25 days (13 doses of PTZ injected) (Postnikova et al 2017, Wang et al 2017. Each animal was placed into an individual plastic cage for observation.…”

Section: Induction Of Ptz Kindlingmentioning

confidence: 99%

Polish Journal of Veterinary Sciences

Kapucu

Üstünova

Akgün-Dar

2020

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Nitric oxide (NO) is known to be a neuromodulator with dual proconvulsive and anticonvulsive action. Valeriana officinalis (VAL) was previously believed to be antiepileptic, but is today known as a sedative and sleep regulator. Seizures may be associated with abnormal electrocardiographic changes and cardiac dysfunction arising from epilepsy may be related with neuronal nitric oxide (nNO). This study was aimed to investigate the effects of the neuronal nitric oxide synthase (nNOS) inhibitor 7-Nitroindazole (7-NI) and VAL on seizure behaviours and electrocardiographic parameters in the pentylentetrazole (PTZ)-kindled seizure model.Wistar rats were randomised into saline control, PTZ-kindled, 7-NI, VAL and VAL+PTZ, 7-NI+PTZ and VAL+7-NI+PTZ groups. Latency, stage, frequency of seizures, blood pressure (BP), heart rate (HR) and corrected QT (QTc) values were evaluated.Frequency and stage of seizures, BP and HR increased, while seizure latency decreased and QTc was prolonged in the PTZ-kindled group. 7-NI and VAL had no effects on BP and HR variables under normal conditions, but ameliorated the seizure stage and frequency of seizures. 7-NI treatment also resulted in a reduction of the increased BP and prolonged QTc values observed in PTZ-kindled rats.Considering these results, QTc prolongation may be used as a predictor for recurrent seizures. 7-NI and VAL exhibited different effects on seizures and ECG variables. 7-NI shows potential as an anticonvulsant drug agent in epileptic patients with cardiac dysfunctions and those additional studies including in-vivo experiments are essential.

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Section: Induction Of Ptz Kindlingmentioning

confidence: 99%

Polish Journal of Veterinary Sciences

Kapucu

Üstünova

Akgün-Dar

2020

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“…Calcium signaling plays a role in many neuron cell processes (Perea et al, 2016) related to protein kinases (Kurniawan et al, 2018), intracellular messenger IP3, SERCA pumps, AMPA and NMDA agonists (Kurniawan, 2015a), affected the synaptic plasticity in the form of LTD (Kurniawan, 2015b). Changes in synaptic plasticity that are influenced by neurotransmitters, glutamate, and AMPA are associated with seizures and epilepsy (Barker-Haliski & Steve White, 2015; Bonansco & Fuenzalida, 2016;Göl et al, 2019;Guli et al, 2016;Han et al, 2016;Husna & Kurniawan, 2018;Jarero-Basulto et al, 2018;Kryukov et al, 2016;Plasticity et al, 2017;T. Yu Postnikova et al, 2017;T Y Postnikova et al, 2017;Zubareva et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Analysis of Modification in Synaptic Plasticity STDP (Spike Timing Dependent Plasticity) Model by Changing Intracellular Calcium Concentration

Diva

Yuniati

2020

kaunia

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Synapses are a junction between neurons that has a plastic property. Synaptic plasticity can be in the form of long-term potentiation (LTP) and long-term depression (LTD). The changes in intracellular calcium concentration can trigger LTP and LTD. Spike Timing Dependent Plasticity (STDP) is a modification of synapses that depend on the spike timing of pre-synapses and post-synapses neuron. In this study, we analyze the effects of intracellular calcium on the STDP phenomena based on Badoual’s model using mechanisms involving parameters such as calcium pumping, AMPA receptors, NMDA receptors, LTP enzymes, and LTD enzymes. In this model, a high calcium concentration will activate the LTP enzyme, while a low calcium concentration will activate the LTD enzyme, each of which will trigger the occurrence of LTP and LTD. The existence of LTP and LTD is the basis for the formation of the STDP. In this model, the parameters can be adjusted to obtain the STDP corresponding to experimental results where LTP is observed in the range between 0 < ∆t < 50ms, while LTD is observed at wider intervals between −150ms < ∆t <0. These parameters are related to the decay time of the NMDA receptor.

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Transient Morphological Alterations in the Hippocampus After Pentylenetetrazole-Induced Seizures in Rats

et al. 2018

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The relationships between seizures, neuronal death, and epilepsy remain one of the most disputed questions in translational neuroscience. Although it is broadly accepted that prolonged and repeated seizures cause neuronal death and epileptogenesis, whether brief seizures can produce a mild but similar effect is controversial. In the present work, using a rat pentylenetetrazole (PTZ) model of seizures, we evaluated how a single episode of clonic-tonic seizures affected the viability of neurons in the hippocampus, the area of the brain most vulnerable to seizures, and morphological changes in the hippocampus up to 1 week after PTZ treatment (recovery period). The main findings of the study were: (1) PTZ-induced seizures caused the transient appearance of massively shrunken, hyperbasophilic, and hyperelectrondense (dark) cells but did not lead to detectable neuronal cell loss. These dark neurons were alive, suggesting that they could cope with seizure-related dysfunction. (2) Neuronal and biochemical alterations following seizures were observed for at least 1 week. The temporal dynamics of the appearance and disappearance of dark neurons differed in different zones of the hippocampus. (3) The numbers of cells with structural and functional abnormalities in the hippocampus after PTZ-induced seizures decreased in the following order: CA1 > CA3b,c > hilus > dentate gyrus. Neurons in the CA3a subarea were most resistant to PTZ-induced seizures. These results suggest that even a single seizure episode is a potent stressor of hippocampal neurons and that it can trigger complex neuroplastic changes in the hippocampus.

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Status epilepticus induced by pentylenetetrazole increases short-term synaptic facilitation in the hippocampus of juvenile rats

Cited by 7 publications

References 10 publications

Polish Journal of Veterinary Sciences

Polish Journal of Veterinary Sciences

Analysis of Modification in Synaptic Plasticity STDP (Spike Timing Dependent Plasticity) Model by Changing Intracellular Calcium Concentration

Transient Morphological Alterations in the Hippocampus After Pentylenetetrazole-Induced Seizures in Rats

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