Statistical optimization for enhanced production of extracellular laccase from Aspergillus sp. HB_RZ4 isolated from bark scrapping

Bhamare, H. M.; Jadhav, H. P.; Sayyed, R. Z.

doi:10.1007/s42398-018-0015-1

Cited by 17 publications

(7 citation statements)

References 23 publications

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“…HB_RZ4 used in this study was obtained from the Department of Biotechnology, SSVPS's Science College, Dhule, Maharashtra, India. It was previously isolated from tree bark scraping [22].…”

Section: Source Of Culturementioning

confidence: 99%

Tree bark scrape fungus: A potential source of laccase for application in bioremediation of non-textile dyes

Sayyed

Bhamare²,

Sapna

et al. 2020

PLoS ONE

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Although laccase has been recognized as a wonder molecule and green enzyme, the use of low yielding fungal strains, poor production, purification, and low enzyme kinetics have hampered its large-scale application. Thus,this study aims to select high yielding fungal strains and optimize the production, purification, and kinetics of laccase of Aspergillus sp. HB_RZ4. The results obtained indicated that Aspergillus sp. HB_RZ4 produced a significantly large amount of laccase under meso-acidophilic shaking conditions in a medium containing glucose and yeast extract. A 25 μM CuSO 4 was observed to enhance the enzyme yield. The enzyme was best purified on a Sephadex G-100 column. The purified enzyme resembled laccase of A. flavus. The kinetics of the purified enzyme revealed high substrate specificity and good velocity of reaction,using ABTS as a substrate. The enzyme was observed to be stable over various pH values and temperatures. The peptide structure of the purified enzyme was found to resemble laccase of A. kawachii IFO 4308. The fungus was observed to decolorize various dyes independent of the requirement of a laccase mediator system. Aspergillus sp. HB_RZ4 was observed to be a potent natural producer of laccase, and it decolorized the dyes even in the absence of a laccase mediator system. Thus, it can be used for bioremediation of effluent that contains non-textile dyes.

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“…HB_RZ4 used in this study was obtained from the Department of Biotechnology, SSVPS's Science College, Dhule, Maharashtra, India. It was previously isolated from tree bark scraping [22].…”

Section: Source Of Culturementioning

confidence: 99%

Tree bark scrape fungus: A potential source of laccase for application in bioremediation of non-textile dyes

Sayyed

Bhamare²,

Sapna

et al. 2020

PLoS ONE

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“…Among the various variables of production media identification, the most significant one is the major process in bioprocess optimization [ 53 , 54 ]. Regarding this, the investigation was initiated using the eight variables viz.…”

Section: Methodsmentioning

confidence: 99%

Statistical Based Bioprocess Design for Improved Production of Amylase from Halophilic Bacillus sp. H7 Isolated from Marine Water

et al. 2021

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Amylase (EC 3.2.1.1) enzyme has gained tremendous demand in various industries, including wastewater treatment, bioremediation and nano-biotechnology. This compels the availability of enzyme in greater yields that can be achieved by employing potential amylase-producing cultures and statistical optimization. The use of Plackett–Burman design (PBD) that evaluates various medium components and having two-level factorial designs help to determine the factor and its level to increase the yield of product. In the present work, we are reporting the screening of amylase-producing marine bacterial strain identified as Bacillus sp. H7 by 16S rRNA. The use of two-stage statistical optimization, i.e., PBD and response surface methodology (RSM), using central composite design (CCD) further improved the production of amylase. A 1.31-fold increase in amylase production was evident using a 5.0 L laboratory-scale bioreactor. Statistical optimization gives the exact idea of variables that influence the production of enzymes, and hence, the statistical approach offers the best way to optimize the bioprocess. The high catalytic efficiency (kcat/Km) of amylase from Bacillus sp. H7 on soluble starch was estimated to be 13.73 mL/s/mg.

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“…HB_RZ4 obtained from the Department of Biotechnology, SSVPS's Science College, Dhule, Maharashtra, India was used in present. It was previously isolated from tree bark scraping [22].…”

Section: Source Of Culturementioning

confidence: 99%

Tree bark scrape fungus: A potential source of laccase for application in bioremediation of non-textile dyes

Bhamare¹,

Sayyed

Marraiki

et al. 2020

Preprint

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Although laccase has been recognized as a wonder molecule, and green enzyme, the use of low yielding fungal strains, poor production, purification, and low enzyme kinetics have hampered its larger-scale applications. Hence the present research was aimed to select high yielding fungal strains and to optimize the production, purification, and kinetics of laccase of Aspergillus sp. HB_RZ4. Aspergillus sp. HB_RZ4 produced a copious amount of laccase on under meso-acidophillic shaking conditions in a medium containing glucose and yeast extract. A 25 µM of CuSO 4 enhanced the enzyme yield. The enzyme was best purified on Sephadex G-100 column. Purified enzyme resembled with the laccase of A. flavus. Kinetics of purified enzyme revealed the high substrate specificity and good velocity of reaction with ABTS as substrate. The enzyme was stable over a wide range of pH and temperature. The peptide structure of the purified enzyme resembled with the laccase of A. kawachii IFO 4308. The fungus decolorized various dyes independent of the requirement of a laccase mediator system (LMS). Aspergillus sp. HB_RZ4 came out as a potent natural producer of laccase, it decolorized the dyes even in absence of LMS and thus can be used for bioremediation.

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Statistical optimization for enhanced production of extracellular laccase from Aspergillus sp. HB_RZ4 isolated from bark scrapping

Cited by 17 publications

References 23 publications

Tree bark scrape fungus: A potential source of laccase for application in bioremediation of non-textile dyes

Tree bark scrape fungus: A potential source of laccase for application in bioremediation of non-textile dyes

Statistical Based Bioprocess Design for Improved Production of Amylase from Halophilic Bacillus sp. H7 Isolated from Marine Water

Tree bark scrape fungus: A potential source of laccase for application in bioremediation of non-textile dyes

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