Statistical Based Bioprocess Design for Improved Production of Amylase from Halophilic Bacillus sp. H7 Isolated from Marine Water

Abstract

Amylase (EC 3.2.1.1) enzyme has gained tremendous demand in various industries, including wastewater treatment, bioremediation and nano-biotechnology. This compels the availability of enzyme in greater yields that can be achieved by employing potential amylase-producing cultures and statistical optimization. The use of Plackett–Burman design (PBD) that evaluates various medium components and having two-level factorial designs help to determine the factor and its level to increase the yield of product. In the present work, we are reporting the screening of amylase-producing marine bacterial strain identified as Bacillus sp. H7 by 16S rRNA. The use of two-stage statistical optimization, i.e., PBD and response surface methodology (RSM), using central composite design (CCD) further improved the production of amylase. A 1.31-fold increase in amylase production was evident using a 5.0 L laboratory-scale bioreactor. Statistical optimization gives the exact idea of variables that influence the production of enzymes, and hence, the statistical approach offers the best way to optimize the bioprocess. The high catalytic efficiency (kcat/Km) of amylase from Bacillus sp. H7 on soluble starch was estimated to be 13.73 mL/s/mg.

Amylase and Cellulase Production from Newly Isolated Bacillus subtilis Using Acid Treated Potato Peel Waste

“…These results are comparable to several previous reports. In fact, the increased liberation of amylase by optimizing different variables of fermentation has been well-documented [ 45 , 46 , 47 , 48 ].…”

A statistical approach to enhance the productivity of Streptomyces baarensis MH-133 for bioactive compounds

“…This method remains viable so long as a reduced number of variables impact the production process [ 32 ]. However, when examining multiple variables, OFAT is insufficient to characterize the collective impact of the various factors at play and requires a substantial number of trials [ 33 ]. Researchers can use the Plackett-Burman design (PBD) and response surface methodology (RSM) as Box-Bhenken design to select the important variables and determine their optimal values, respectively, thereby overcoming these limitations [ [34] , [35] ].…”

Biotechnological potentials of halophilic microorganisms and their impact on mankind

“… pH 7.0, temp 45 °C, 3 M NaCl, 0.2 M MgCl 2 Specific activity of 2975 U/mg Food, textile, brewing industry [ 37 ] Bacillus sp. pH 8.0, temp 40 °C, K m value 5.1 mg/mL and V max value 116.28 μM/min/mL 130.53 ± 2.0 U/mL [ 38 ] Lipase Haloferax mediterranei pH 7.0 and 60 °C , K m and V max for HML toward olive oil were 1.01 mM and 1195 U/mg, respectively 50 U/mL Detergent, textile, pharmaceuticals [ 39 ] Marinobacter sp. 2.5–3.5 M NaCl, pH 8.5, temperature 55 °C 49.5 U mL −1 [ 40 ] Chromohalobacter canadensis 4 M NaCl, 50 °C and pH 7 60.57 U/mg [ 41 ] Streptomyces sp.…”