Statistical Optimization for Coproduction of Chitinase and Beta 1, 4-Endoglucanase by Chitinolytic Paenibacillus elgii PB1 Having Antifungal Activity

Philip, N.; Koteshwara, Ananthamurthy; Kiran, G. Aditya; Raja, S.; Subrahmanyam, V. M.; Chandrashekar, H. Raghu

doi:10.1007/s12010-020-03235-8

Cited by 18 publications

(11 citation statements)

References 36 publications

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“…CH-2 51 Fish market soil – 3.1 (PBD and CCD) Paenibacillus sp. 44 Coastal soil 3.84 20.01 (PBD and CCD) Paenibacillus elgii 52 Marine soil 3.157 24.53 Pseudomonas fluorescens strain HN1205 46 Coastal soil – 1.03 U/mL(PBD and CCD) Paenibacillus sp. D1 38 – 93.2 (PBD and CCD) …”

Section: Resultsmentioning

confidence: 99%

Bioconversion of chitin and concomitant production of chitinase and N-acetylglucosamine by novel Achromobacter xylosoxidans isolated from shrimp waste disposal area

Shanmugam

Sadaiappan

Aruni

et al. 2020

Sci Rep

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Marine pollution is a significant issue in recent decades, with the increase in industries and their waste harming the environment and ecosystems. Notably, the rise in shellfish industries contributes to tons of shellfish waste composed of up to 58% chitin. Chitin, the second most ample polymer next to cellulose, is insoluble and resistant to degradation. It requires chemical-based treatment or enzymatic hydrolysis to cleave the chitin polymers. the chemical-based treatment can lead to environmental pollution, so to solve this problem, enzymatic hydrolysis is the best option. Moreover, the resulting biopolymer by-products can be used to boost the fish immune system and also as drug delivery agents. Many marine microbial strains have chitinase producing ability. Nevertheless, we still lack an economical and highly stable chitinase enzyme for use in the industrial sector. So we isolate a novel marine bacterial strain Achromobacter xylosoxidans from the shrimp waste disposal site using chitin minimal medium. Placket-Burman and central composite design statistical models for culture condition optimisation predicted a 464.2 U/ml of chitinase production. The culture conditions were optimised for maximum chitinase production recording up to 467 U/ml. This chitinase from the A. xylosoxidans was 100% active at an optimum temperature of 45 °C (withstand up to 55 °C) and pH 8 with 80% stability. The HPLC analysis of chitinase degraded shellfish waste reveals a major amino acid profile composition-arginine, lysine, aspartic acid, alanine, threonine and low levels of isoleucine and

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Section: Resultsmentioning

confidence: 99%

Bioconversion of chitin and concomitant production of chitinase and N-acetylglucosamine by novel Achromobacter xylosoxidans isolated from shrimp waste disposal area

Shanmugam

Sadaiappan

Aruni

et al. 2020

Sci Rep

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“…As a number of studies have shown [79,97,98], such a combination of chitinases with different substrate ranges can be successfully supplemented by the action of other hydrolytic enzymes (proteases and glucanases) [87,88,[96][97][98], which use molecules performing the role of structural elements of the fungal cell wall and membranes as substrates. However, it should be emphasized that not all chitinases known today [100] can be used as antifungal agents since the diverse structure of fungal polysaccharides is characterized by the presence of various glycoside residues of different lengths and often does not correspond to the preferences of those substrate specificities possessed by most of these enzymes.…”

Section: Antifungal Enzymes Using Cell Structural Components Of Fungi...mentioning

confidence: 99%

“…Nucleases hydrolyzing the RNA and DNA of fungi attract particular attention among enzymes that have antifungal activity [93,94,99]. The use of several nucleases at once [96][97][98] or nuclease in combination with glucanase [99] leads to the fact that not only is the growth of fungal cells stopped, but membrane destruction (permeabilization and depolymerization) is observed. This reduces the membrane potential of mitochondria, leading to the degradation of target cell nucleic acids and the death of microbial cells.…”

Section: Antifungal Enzymes Using Cell Structural Components Of Fungi...mentioning

confidence: 99%

Metal Nanomaterials and Hydrolytic Enzyme-Based Formulations for Improved Antifungal Activity

Lyagin

Aslanli

Domnin

et al. 2023

IJMS

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Active research of metal-containing compounds and enzymes as effective antifungal agents is currently being conducted due to the growing antifungal resistance problem. Metals are attracting special attention due to the wide variety of ligands that can be used for them, including chemically synthesized and naturally obtained variants as a result of the so-called “green synthesis”. The main mechanism of the antifungal action of metals is the triggering of the generation and accumulation of reactive oxygen species (ROS). Further action of ROS on various biomolecules is nonspecific. Various hydrolytic enzymes (glucanases and proteases), in turn, exhibit antifungal properties by affecting the structural elements of fungal cells (cell walls, membranes), fungal quorum sensing molecules, fungal own protective agents (mycotoxins and antibiotics), and proteins responsible for the adhesion and formation of stable, highly concentrated populations in the form of biofilms. A wide substrate range of enzymes allows the use of various mechanisms of their antifungal actions. In this review, we discuss the prospects of combining two different types of antifungal agents (metals and enzymes) against mycelial fungi and yeast cells. Special attention is paid to the possible influence of metals on the activity of the enzymes and the possible effects of proteins on the antifungal activity of metal-containing compounds.

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“…Predicting optimal culture conditions using a statistical approach with the Plackett–Burman design (PBD) increased the production of chitinase and beta 1,4-endoglucanase by P . elgii PB1 and also enhanced antibacterial activity [ 173 ]. The use of PBD shows production of the insecticide avermectin B1 from Streptomyces avermitilis 14-12 to be positively influenced by carbon and nitrogen sources [ 174 ].…”

Section: Secondary Metabolites and Cell Preparations For Commercial B...mentioning

confidence: 99%

Root-Associated Bacteria Are Biocontrol Agents for Multiple Plant Pests

Lee¹,

Anderson

Kim

2022

Microorganisms

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Biological control is an important process for sustainable plant production, and this trait is found in many plant-associated microbes. This study reviews microbes that could be formulated into pesticides active against various microbial plant pathogens as well as damaging insects or nematodes. The focus is on the beneficial microbes that colonize the rhizosphere where, through various mechanisms, they promote healthy plant growth. Although these microbes have adapted to cohabit root tissues without causing disease, they are pathogenic to plant pathogens, including microbes, insects, and nematodes. The cocktail of metabolites released from the beneficial strains inhibits the growth of certain bacterial and fungal plant pathogens and participates in insect and nematode toxicity. There is a reinforcement of plant health through the systemic induction of defenses against pathogen attack and abiotic stress in the plant; metabolites in the beneficial microbial cocktail function in triggering the plant defenses. The review discusses a wide range of metabolites involved in plant protection through biocontrol in the rhizosphere. The focus is on the beneficial firmicutes and pseudomonads, because of the extensive studies with these isolates. The review evaluates how culture conditions can be optimized to provide formulations containing the preformed active metabolites for rapid control, with or without viable microbial cells as plant inocula, to boost plant productivity in field situations.

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Statistical Optimization for Coproduction of Chitinase and Beta 1, 4-Endoglucanase by Chitinolytic Paenibacillus elgii PB1 Having Antifungal Activity

Cited by 18 publications

References 36 publications

Bioconversion of chitin and concomitant production of chitinase and N-acetylglucosamine by novel Achromobacter xylosoxidans isolated from shrimp waste disposal area

Bioconversion of chitin and concomitant production of chitinase and N-acetylglucosamine by novel Achromobacter xylosoxidans isolated from shrimp waste disposal area

Metal Nanomaterials and Hydrolytic Enzyme-Based Formulations for Improved Antifungal Activity

Root-Associated Bacteria Are Biocontrol Agents for Multiple Plant Pests

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