Statistical optimization and kinetic modeling of xylanase production by Arthrobacter sp.

Abstract

Xylanases are hydrolytic enzymes which randomly cleave the β-1, 4 backbone of the complex plant cell wall polysaccharide xylan. Diverse forms of these enzymes exist, displaying varying folds, mechanisms of action, substrate specificities, hydrolytic activities, and physicochemical characteristics. This article focuses on the fermentative production of xylanase from Arthrobacter sp. and the factors affecting the production. The batchsubmerged fermentation was carried out in a 250-ml Erlenmeyer flask. The inoculum size of 5% v/v, initial pH of 7.0, and shaking speed of 150 rpm at 32 • C were maintained for the optimization of medium components. The cell growth, xylanase activity, cellulase activity, protein, and pH were determined at regular time intervals. Plackett-Burman statistical methodology was adopted to study the effect of media components on xylanase production. The effect of temperature and pH was studied using optimized medium composition determined by Plackett-Burman experimental design. The maximum xylanase activity of 4.0 U ml −1 at 84 h at the optimum temperature of 35 • C and pH of 7.0 was obtained. 

Optimization of bio-cement production from cement kiln dust using microalgae

“…Briefly, RSM is the most well-known statistical, fully randomized and bias free method in finding the optimum conditions using quadratic regression models. RSM has been used widely for optimization and modelling in different aspects including bio-synthesis materials such as biodiesel, bio-cement and various bacterial/microalgal media for high yield such as lipases, chitinases, xylanases, keratinases, lipid productivity including CO 2 biofixation [[18], [19], [20], [21], [22], [23], [24], [25]]. Dhami et al [21] optimized the medium components using central composite design (CCD) to heighten urease yield and explore its influence on MICP thereafter.…”

Studies on the effect of pH, temperature and metal ions on the production of pectinase from tamarind kernel powder by submerged fermentation using Aspergillus foetidus (NCIM 505)

“…Testa-free tamarind seeds were ground to a fine powder and then used as substrate for submerged fermentation. [16,17] …”

Development of Medium and Kinetic Modeling for Enhanced Production of Cutinase from Pseudomonas cepacia NRRL B-2320

“…To the best of our knowledge this is the first report on the kinetic modeling fermentation profile during cutinase production. Few reports are available on lipase [22] and xylanase [23] production kinetics where production was found mostly growth associated. The coefficient of determination, R 2 , is a measure of the strength of the linear relationship between the experimental and predicted values.…”