Statistical media optimization and cellulase production from marine Bacillus VITRKHB

Singh, Kulbir; Richa, K.; Bose, Himadri; Karthik, Loganathan; Kumar, Gaurav; Rao, Kokati Venkata Bhaskara

doi:10.1007/s13205-013-0173-x

Cited by 29 publications

(13 citation statements)

References 29 publications

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“…Recently, RSM was applied for optimized production of cellulases (Singh et al 2014; Premalatha et al 2015), fibrinolytic enzyme (Majumdar et al 2015) and xylanase (Khusro et al 2016). The optimum concentration of individual process parameters considering the interactive effect were elucidated using RSM.…”

Section: Resultsmentioning

confidence: 99%

Bio-prospecting of cuttle fish waste and cow dung for the production of fibrinolytic enzyme from Bacillus cereus IND5 in solid state fermentation

Devadhasan¹,

Arumugaperumal

Muthulakshmi

et al. 2016

3 Biotech

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The process parameters governing the production of fibrinolytic enzyme in solid state fermentation employing Bacillus cereus IND5 and using cuttle fish waste and cow dung substrate were optimized. The pH value of the medium, moisture content, sucrose, casein and magnesium sulfate were considered for two-level full factorial design and pH, casein and magnesium sulfate were identified as the important factors for fibrinolytic enzyme production. Central composite design was applied to investigate the interactive effect among variables (pH, casein and magnesium sulfate) and response surface plots were created to find the pinnacle of process response. The optimized levels of factors were pH 7.8, 1.1% casein and 0.1% magnesium sulfate. Enzyme production was increased 2.5-fold after statistical approach. The enzyme was purified up to a specific activity of 364.5 U/g proteins and its molecular weight was 47 kDa. It was stable at pH 8.0 and was highly active at 50 °C. The mixture of cuttle fish waste and cow dung could find great application as solid substrate for the production of fibrinolytic enzyme.

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Section: Resultsmentioning

confidence: 99%

Bio-prospecting of cuttle fish waste and cow dung for the production of fibrinolytic enzyme from Bacillus cereus IND5 in solid state fermentation

Devadhasan¹,

Arumugaperumal

Muthulakshmi

et al. 2016

3 Biotech

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“…Various reports show that cellulase and xylanase induction seems to be species-specific in different bacterial strains. For instance, studies have reported that cellulase production by Bacillus VITRKHB was optimal in fermentation media supplemented with xylose as the carbon source; on the other hand, the best carbon source for cellulase production by B. subtilis and B. pumilus EWBCM1 was lactose and galactose, respectively [48,65,66]. Furthermore, xylose was the preferred carbon for xylanase production by Arthrobacter sp.…”

Section: Effect Of Carbon Source Inducers On Cellulase and Xylanase Pmentioning

confidence: 99%

Classical Optimization of Cellulase and Xylanase Production by a Marine Streptomyces Species

2016

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Cellulase and xylanase are in high demand for application in several industrial processes, consequently necessitating the bioprospecting and manipulation of microbes for novel and greater enzyme productivity. This study reports on the optimal conditions for cellulase and xylanase production by a marine bacterial isolate from Nahoon beach sediment, via the classical process of one variable per time. Furthermore, the inducing effect of mono-and polysaccharides on enzyme production was investigated. The 16S rDNA gene sequence analysis clearly assigned the isolate to the genus Streptomyces, and was deposited at the GenBank under the accession number KU171373. Cellulase and xylanase production was optimal at the following conditions: pH 6 and 8, incubation temperature of 40 and 35 • C, and agitation speed of 100 and 150 rpm, respectively. Under optimum conditions, 0.26 U/mL and 18.54 U/mL activities were attained at 60 and 48 h with specific productivity of 205 and 7417 U/g for cellulase and xylanase, respectively. Xylanase production was induced by the entire mono-and polysaccharides tested, while cellulase production was induced by some. The results from this study signify the resourcefulness of the Streptomyces strain for production of cellulase and xylanase of industrial importance.

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“…Now-a-days microorganisms are being used due to their broad biochemical diversity, feasibility of mass culture, regular supply due to absence of seasonal variations, ease of genetic manipulation and high degree of stability than enzymes derived from plants and animals. 4 Several industrial enzymes are derived from terrestrial sources. Whereas marine environment which encompasses about 71 % of the earth's surface and a vast resource of useful enzymes has remain unexplored.…”

Section: Marine Microbial Enzymesmentioning

confidence: 99%

“…Earlier reports suggest their salttolerating capacity of above 1.7M, hyperthermostability (80-108˚C), barophilicity (60MPa), cold adaptivity, and alkali stability. 4 Marine microorganisms take active part in the mineralization of complex organic matter through degradative pathways of their metabolism in the marine environments and contribute to the secondary production in sea. Microbial hydrolysis of organic matter plays an important role in substrate turnover in aquatic environments.…”

Section: Marine Microbial Enzymesmentioning

confidence: 99%

Immobilisation of marine pectinase on nylon 6,6

Damle¹,

Badhe²,

Mahajan³

et al. 2018

JTEFT

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Industries all over the world face an uphill battle in meeting the material needs of a rising global population at a time when natural resources are dwindling. By replacing harsh chemicals with biologically derived alternatives and at the same time minimizing water and energy use, sustainability is at heart of the world's growth. Perhaps nowhere has the impact of modern biotechnology been more widely felt than in the application of industrial enzymes? Enzymes represent lucrative option to substitute harsh chemicals used in processing operations in different industries. Enzymes being target specific and biodegradable are looked upon as lucrative source for providing clean and green substitute to harsh chemicals used in different industries. Pectinase is one of the enzymes that acts as alternative to chemicals used in different textile processing operations. The current demands of the world's biotechnological industries are enhancement in enzyme productivity and development of novel techniques for increasing their shelf life. These requirements are inevitable to facilitate large-scale and economically feasible formulation. Immobilizing pectinase by suitable technique allows its reusability and in turn will make pectinase economical. In this research, immobilization of pectinase on knitted nylon 6,6 fabric is studied which showed immobilized biocatalyst could be used successively; effectively upto three cycles.

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Statistical media optimization and cellulase production from marine Bacillus VITRKHB

Cited by 29 publications

References 29 publications

Bio-prospecting of cuttle fish waste and cow dung for the production of fibrinolytic enzyme from Bacillus cereus IND5 in solid state fermentation

Bio-prospecting of cuttle fish waste and cow dung for the production of fibrinolytic enzyme from Bacillus cereus IND5 in solid state fermentation

Classical Optimization of Cellulase and Xylanase Production by a Marine Streptomyces Species

Immobilisation of marine pectinase on nylon 6,6

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