Statistical Examination of a Rapid Method for the Determination of Chlorinated Hydrocarbon Residues in Human Blood

Abstract

An orderly examination of the laboratory parameters involved in the recovery of the chlorinated hydrocarbon residue, p,p′-DDE, from human blood serum by the method of Dale, Curley, and Cueto is reported. The age of the serum, amount of hexane used for extraction, duration of mixing time, and temperature were varied; the results were examined statistically to discern the possible effects. All these factors had a statistically significant effect upon the results of the analytical quantitation performed by GLC. The aging time of these rum and the amount of hexane used for the extraction as well as the aging time and duration of mixing time were intercorrelated. Modification for use of the published procedures based upon these findings is suggested.