Statistical approach in alginate membrane formulation for cell encapsulation in a GMP-based cell factory

Villani, Simona; Marazzi, Mario; Bucco, Massimo; Faustini, M.; Klinger, Marco; Gaetani, Paolo; Crovato, F.; Vigo, D.; Caviggioli, F.; Torre, Maria Luisa

doi:10.1016/j.actbio.2008.01.007

Cited by 20 publications

(16 citation statements)

References 24 publications

(24 reference statements)

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“…These broad applications emphasize the need to develop and understand the dynamics of cell encapsulation and isolation processes. There are various technologies reported to encapsulate cells in media and hydrogel droplets [15], [16], [17]. The conventional inkjet printing systems were adapted [15] as tools to encapsulate cells in droplets and pattern these cell-encapsulating droplets [18].…”

Section: Introductionmentioning

confidence: 99%

“…Several statistical approaches were presented for encapsulation using various technologies including cell encapsulation in polymers [16] and emulsions [17], cell separation with micro-well arrays [30], fluorescence-activated droplet sorting [26], and droplet generation using microfluidics [27], [28], [29]. Villani et al presented a statistical approach in alginate membrane formulation for cell encapsulation [16]. Using a passive tool, e.g., a microwell template, Love et al experimentally analyzed manual cell loading efficiency for microwells with homogeneous cell types [30].…”

Section: Introductionmentioning

confidence: 99%

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Statistical Modeling of Single Target Cell Encapsulation

et al. 2011

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High throughput drop-on-demand systems for separation and encapsulation of individual target cells from heterogeneous mixtures of multiple cell types is an emerging method in biotechnology that has broad applications in tissue engineering and regenerative medicine, genomics, and cryobiology. However, cell encapsulation in droplets is a random process that is hard to control. Statistical models can provide an understanding of the underlying processes and estimation of the relevant parameters, and enable reliable and repeatable control over the encapsulation of cells in droplets during the isolation process with high confidence level. We have modeled and experimentally verified a microdroplet-based cell encapsulation process for various combinations of cell loading and target cell concentrations. Here, we explain theoretically and validate experimentally a model to isolate and pattern single target cells from heterogeneous mixtures without using complex peripheral systems.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Statistical Modeling of Single Target Cell Encapsulation

et al. 2011

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“…[2][3][4][5][6][7] In fact, adipose tissue has been identified as a rich, easy-to-reach source of mesenchymal stem cells (MSCs), able to differentiate into adipocytes, chondrocytes, osteoblasts, myocytes, cardiomyocytes, hepatocytes, epithelial cells, and endothelial cells. [8][9][10][11] Some authors report that also neuronal/glial differentiation can occur from the morphological but not physiological point of view.…”

Section: Introductionmentioning

confidence: 99%

Nonexpanded Mesenchymal Stem Cells for Regenerative Medicine: Yield in Stromal Vascular Fraction from Adipose Tissues

Faustini

Bucco

Chlapanidas

et al. 2010

Tissue Engineering Part C: Methods

108

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The adipose-derived stromal vascular fraction (SVF) represents a rich source of mesenchymal cells, potentially able to differentiate into adipocytes, chondrocytes, osteoblasts, myocytes, cardiomyocytes, hepatocytes, and neuronal, epithelial, and endothelial cells. These cells are ideal candidates for use in regenerative medicine, tissue engineering, including gene therapy, and cell replacement cancer therapies. In this work, we aimed to the optimization of the adipose SVF-based therapy, and the effect of the collection site, surgical procedure, and tissue processing techniques on SVF yield was evaluated in terms of cell recovery and live cells, taking into account the effect of gender, age, and body mass index. Adipose tissue samples were recovered from 125 informed subjects (37 males and 88 females; mean age: 51.31 years; range: 15-87 years), and digested in different condition with collagenase. A multivariate linear model put in evidence that in males the best collection site in terms of yield is located in the abdomen, whereas in females the biopsy region do not influence cell recovery; the collection technique, the age, and the body mass index of donor seem not to influence the cell yield. The tissueprocessing procedures strongly modify the yield and the vitality of cells: a collagenase concentration of 0.2% and a digestion time of 1 h could be chosen as the best operating conditions.

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“…Facility set up [29]. c. Cell encapsulation [38,39]. Isolation and expansion of hESCs [30], mesenchymal stromal cells [31][32][33][34][35][36], and cord blood cells [37].…”

Section: Cell Therapymentioning

confidence: 99%