Statistical analysis of the distribution of the antibody levels to Mycobacterium bovis antigenes for bovine tuberculosis diagnostics

Redchuk, Taras A.; Короткевич, Н. В.; Kaberniuk, Andrii A.; Oliinyk, E. S.; Labyntsev, А. J.; Romaniuk, S. I.; Dv, Kolibo; Busol, V. A.; Комісаренко, С. В.

doi:10.3103/s009545271005004x

Cited by 6 publications

(6 citation statements)

References 30 publications

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“…Likewise, the secretion of MPT63 and MPT64 is increased in active TB. The ortholog MPB63 from M. bovis only differs in nucleotide sequence from MTB MPT63 but is not distinct in function [ 42 ]. MPT64, the gene locus in RD2, is not expressed in the BCG strain because of the lack of RD2 region [ 43 ].…”

Section: Discussionmentioning

confidence: 99%

Multi-Functional MPT Protein as a Therapeutic Agent against Mycobacterium tuberculosis

et al. 2021

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Mycobacterium tuberculosis (MTB), the causative agent of tuberculosis (TB), avoids the host immune system through its virulence factors. MPT63 and MPT64 are the virulence factors secreted by MTB which regulate host proteins for the survival and proliferation of MTB in the host. Here, we found that MPT63 bound directly with TBK1 and p47phox, whereas MPT64 interacted with TBK1 and HK2. We constructed a MPT63/64-derived multifunctional recombinant protein (rMPT) that was able to interact with TBK1, p47phox, or HK2. rMPT was shown to regulate IFN-β levels and increase inflammation and concentration of reactive oxygen species (ROS), while targeting macrophages and killing MTB, both in vitro and in vivo. Furthermore, the identification of the role of rMPT against MTB was achieved via vaccination in a mouse model. Taken together, we here present rMPT, which, by regulating important immune signaling systems, can be considered an effective vaccine or therapeutic agent against MTB.

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Section: Discussionmentioning

confidence: 99%

Multi-Functional MPT Protein as a Therapeutic Agent against Mycobacterium tuberculosis

et al. 2021

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“…From the biotechnological position, the starting point for any serological method is the immune response. And hence one of the most important fundamental grounds of the creation of serological diagnostic kits is the understanding of the regularity and features of the immune response against the certain antigen [8][9][10][11]. The most appropriate is specific monoclonal antibodies (McAbs), as they, unlike polyclonal antibodies are characterized by exceptional specificity, homogeneity and possibility of obtaining in unlimited quantities.…”

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confidence: 99%

Different mice inbred strains humoral immune response against human prostate-specific antigen

Galkin¹,

Komar²,

Besarab³

2019

Ukr.Biochem.J.

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The aim of the study was an investigation of humoral immune response to prostate-specific antigen (PSA) of different mice inbred strains for further development of recommendations for appropriate immunization schemes for monoclonal antibodies (mcabs) obtaining. The study was conducted using: Balb/c and NZB mice; PSA from human sperm (as immunogen). In case of booster immunization immunogen was previously diluted to the desired concentration (10 or 30 µg per 100 µl) in saline, and injected in the tail vein or intraperitoneally. In case of other immunizations, we prepared emulsion solution of immunogen with adjuvant to final concentration of 10 or 30 µg per 100 µl: PSa was dissolved in saline, the same volume of adjuvant was added, and mixture was thoroughly mixed to form a stable emulsion. When subcutaneous administration, total dose of 100 µl was divided into two equal parts and injected in the hind paw of mice. Intraperitoneal immunization was performed by single administration of 100 µl of emulsion. The level of specific antibodies was determined by titration of blood sera from animals in indirect elISa. Series of experiments were conducted to determine the level of humoral response of mice of different inbred strains (Balb/c and NZB) for multi-stage immunization with different duration with different amounts of PSA (10 and 30 μg), with different immunoglobulin administration (intraperitoneal and subcutaneously), and with different adjuvants (Freund's complete and incomplete adjuvants, FCA/FIA). Final booster immunization at a dose of 30 μg was performed either in the same manner as the previous administration of the immunogen, or intravenously in the physio logical saline solution. Dependencies of the humoral immune response of Balb/c and NZB mice against PSa on the route of administration of immunogen, the dose and duration of immunization were established. It was shown that intraperitoneal administration provided formation of higher titers of specific antibodies in case of both mice strains. Balb/c mice lines more rapidly responded to PSa, than an NZB mice (for all investigated schemes immunization). It was shown that the most effective immunization scheme was three times intraperitoneal administration with 10 µg of PSA for 8 weeks (the first immunization with FCA, and the rest-with FIA) and booster immunogen intravenous administration in saline solution.

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“…The present study measured the antibody response using ELISA test to fusion protein MPT83-MPT63 obtained previously [5]. These two antigens are synthesized in abundance in m. tuberculosis or m. bovis strains [6].…”

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confidence: 99%

“…The use of these proteins is advisable because they are absent in the genome of most not pathogenic mycobacteria [6].…”

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confidence: 99%

Specificity and sensitivity of the new test for serological evaluation of tuberculosis using MPT83-MPT63 fusion antigen

Siromolot¹,

Chudina²,

Danilova³

et al. 2018

Ukr.Biochem.J

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the aim of this work was to characterize the experimental test-system based on mPt83-mPt63 fusion antigen with reference commercial diagnostics and investigate the basic characteristics of enzyme-linked immunosorbent assay (ELISA) tests such as specificity and sensitivity. In addition, we investigated the correlation of biochemical and immunological parameters of blood samples of patients with tuberculosis, which was correctly diagnosed by those test-systems. It was shown that the developed test-system match the existing ones regarding the criteria of reliability and the basic requirements of elISA kits. recommendations for the testing of serum samples and indications for the use of the proposed serological diagnostic methods has been suggested. k e y w o r d s: diagnostic of tuberculosis, recombinant protein, elISA.

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Statistical analysis of the distribution of the antibody levels to Mycobacterium bovis antigenes for bovine tuberculosis diagnostics

Cited by 6 publications

References 30 publications

Multi-Functional MPT Protein as a Therapeutic Agent against Mycobacterium tuberculosis

Multi-Functional MPT Protein as a Therapeutic Agent against Mycobacterium tuberculosis

Different mice inbred strains humoral immune response against human prostate-specific antigen

Specificity and sensitivity of the new test for serological evaluation of tuberculosis using MPT83-MPT63 fusion antigen

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