Specificity and sensitivity of the new test for serological evaluation of tuberculosis using MPT83-MPT63 fusion antigen

Siromolot, A. A.; Chudina, T. О.; Danilova, I. S.; Rekalova, О. M.; Kolybo, Denys; Комісаренко, С. В.

doi:10.15407/ubj90.06.041

Cited by 2 publications

(2 citation statements)

References 9 publications

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“…The experimental test system "IB-Chem Anti-Mycobacterium tuberculosis" is not inferior to the existing diagnostic serological tests in terms of reliability and informativeness; it meets the basic requirements for the use of ELISA test kits in practice. Previous studies have shown that the sensitivity of this test system was 70% and specifi city was 95.5% [21].…”

mentioning

confidence: 72%

“…In a previously conducted study, indicators of general specifi city and sensitivity of the newly developed test system "IB-Chem Anti-Mycobacterium tuberculosis" and two analogous commercial test systems for detecting IgG antibodies to M.tuberculosis were analysed (test system #1 (Ukraine), test system #2 (Russia)) [21]. The results showed that both commercial diagnostic kits have the same sensitivity rate at 55% (compared to 70% for "IB-Chem Table 3.…”

Section: Discussionmentioning

confidence: 99%

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Detection of Specific Antibodies Using Mpt83-Mpt63 Fusion Protein in Patients With Destructive Pulmonary Tuberculosis

Rekalova

Panasiukova²,

Pohrebna³

et al. 2022

PSSS MS

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Introduction. The development of eff ective test systems for tuberculosis (TB) diagnosis and the study of factors infl uencing their sensitivity and specifi city constitute urgent tasks given the unstable epidemiological situation with TB in Ukraine and the world. The study aimed to determine the clinical and laboratory features of pulmonary TB that may aff ect the sensitivity of IgG TB antibody detection in human serum by the immuno-enzyme test system "IB-Chem Anti-Mycobacterium tuberculosis", which was developed at the Palladin Institute of Biochemistry of the NAS of Ukraine and uses recombinant fusion protein MPT83-MPT63 as an antigen. Methods. Sera of 62 patients with chemoresistant pulmonary TB were tested for TB antibodies using an indirect enzyme-linked immunosorbent assay. Dependence of the obtained results on clinical symptoms and signs of pulmonary TB, the frequency of diff erent types of antibiotic resistance, cellular and biochemical parameters of blood were analysed. Results. The sensitivity of the immuno-enzyme test system "IB-Chem Anti-Mycobacterium tuberculosis" for detecting human IgG TB antibodies using recombinant fusion protein MPT83-MPT63 was found to constitute 71.0%; it reached 81.3% in patients with destructive pulmonary TB. Conclusion. The developed test system should primarily be used for the TB diagnosis in patients with destructive changes in the lungs where the diagnostic effi ciency of this test system reaches high indicators.

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mentioning

confidence: 72%

Section: Discussionmentioning

confidence: 99%

Detection of Specific Antibodies Using Mpt83-Mpt63 Fusion Protein in Patients With Destructive Pulmonary Tuberculosis

Rekalova

Panasiukova²,

Pohrebna³

et al. 2022

PSSS MS

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Triple Immunochromatographic System for Simultaneous Serodiagnosis of Bovine Brucellosis, Tuberculosis, and Leukemia

et al. 2019

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An immunochromatographic test system has been developed for the simultaneous rapid multiplex serodiagnostics of bovine brucellosis, tuberculosis, and leukemia. The test system is based on the use of a conjugate of gold nanoparticles with the chimeric protein Cysteine-A/G and three analytical zones with immobilized pathogen antigens: Brucella abortus lipolysaccharide, recombinant proteins MPB64 and MPB83-MPB63 of Mycobacterium bovis, and recombinant protein p24 of the bovine leukemia virus. Prototypes of the test system were tested on 98 samples of sera from healthy and infected animals. The diagnostic sensitivity of the developed test system was 92% for brucellosis, 92% for tuberculosis, and 96% for leukemia. False positive test results were not observed.

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Specificity and sensitivity of the new test for serological evaluation of tuberculosis using MPT83-MPT63 fusion antigen

Cited by 2 publications

References 9 publications

Detection of Specific Antibodies Using Mpt83-Mpt63 Fusion Protein in Patients With Destructive Pulmonary Tuberculosis

Detection of Specific Antibodies Using Mpt83-Mpt63 Fusion Protein in Patients With Destructive Pulmonary Tuberculosis

Triple Immunochromatographic System for Simultaneous Serodiagnosis of Bovine Brucellosis, Tuberculosis, and Leukemia

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