Triple Immunochromatographic System for Simultaneous Serodiagnosis of Bovine Brucellosis, Tuberculosis, and Leukemia

Barshevskaya, Lyubov V; Sotnikov, Dmitriy V.; Zherdev, Anatoly V.; Khassenov, Bekbolat; Baltin, Kairat; Eskendirova, Saule; Mukanov, Kassym K.; Mukantayev, Kanatbek; Dzantiev, Boris B.

doi:10.3390/bios9040115

Cited by 5 publications

(3 citation statements)

References 34 publications

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“…The results of this study are not meant to challenge the current diagnosis for bovine tuberculosis and the performance of the current modified Z -N direct smear microscopy method as both a screening and confirmatory test. Our results provide some relevant information to guide the choice of potential laboratory tests for future use [31][32][33]. An exercise, that could be useful as part of the global strategy for elimination of tuberculosis.…”

Section: Discussionmentioning

confidence: 90%

Strengthening the Monitoring and Surveillance System for Bovine Tuberculosis in Post Mortem Material from Slaughtered Cattle in Eswatini: A Review

Dlamini

Mudyanavana²,

Mdluli³

et al. 2021

AJAAR

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The main objective of this review study was to examine the validity of test results generated by using the Ziehl-Neelsen smear microscopy as the sole diagnostic tool for the monitoring and surveillance of bovine tuberculosis in cattle slaughtered in Eswatini. Methods: A retrospective analysis of available laboratory data of beef carcass condemnations for a study period from 2014 to 2018 was carried out. Literature was reviewed to find out potential sample preparation and concentration methods to improve the sensitivity of current Z-N smear microscopy. Results: The limited literature reviewed in this study has briefly highlighted (i) the limitations of using the Z-N direct smear microscopy as a screening and confirmatory laboratory test for bovine tuberculosis suspected carcasses, (ii) that the Z-N smear microscopy method may be inadequate for identifying and differentiating other acid fast bacilli co-existing in a M. bovis infection, (iii) that concentration of lymph node aspirate samples may improve the sensitivity of acid fast bacilli smear microscopy, (iv) that strategic deployment of ancillary laboratory tests, such as the Rapid diagnostic tests (RDT) and the Polymerase chain reaction test (PCR) alongside the Z-N smear microscopy may achieve an adequate level of diagnostic test performance. Conclusion: In order to strengthen the surveillance and monitoring of bovine tuberculosis in the country, it is necessary to consider techniques for the concentration of bovine tuberculosis suspect lymph node samples, to use Rapid diagnostic test Kits in parallel to the Z-N direct smear microscopy.

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Section: Discussionmentioning

confidence: 90%

Strengthening the Monitoring and Surveillance System for Bovine Tuberculosis in Post Mortem Material from Slaughtered Cattle in Eswatini: A Review

Dlamini

Mudyanavana²,

Mdluli³

et al. 2021

AJAAR

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“…Lateral flow immunoassay (LFIA) has been widely used in recent years as a mainstream instant diagnostic method due to its advantages of easy operation, low material cost, and fast detection speed [ 1 , 2 ]. It has been widely applied in medical diagnosis, food safety, environmental monitoring, and other fields [ 3 , 4 , 5 , 6 , 7 , 8 ]. Large molecular analytes such as proteins are typically tested using sandwich assays, while small molecule analytes such as drugs of abuse are analyzed using competitive or inhibitory assays [ 9 , 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Research on the Flow Characteristics and Reaction Mechanisms of Lateral Flow Immunoassay under Non-Uniform Flow

Zhao,

Zhang,

Niu

et al. 2024

Sensors

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Lateral flow immunoassay (LFIA) is extensively utilized for point-of-care testing due to its ease of operation, cost-effectiveness, and swift results. This study investigates the flow dynamics and reaction mechanisms in LFIA by developing a three-dimensional model using the Richards equation and porous media transport, and employing numerical simulations through the finite element method. The study delves into the transport and diffusion behaviors of each reaction component in both sandwich LFIA and competitive LFIA under non-uniform flow conditions. Additionally, the impact of various parameters (such as reporter particle concentration, initial capture probe concentrations for the T-line and C-line, and reaction rate constants) on LFIA performance is analyzed. The findings reveal that, in sandwich LFIA, optimizing parameters like increasing reporter particle concentration and initial capture probe concentration for the T-line, as well as adjusting reaction rate constants, can effectively enhance detection sensitivity and broaden the working range. Conversely, in competitive LFIA, the effects are inverse. This model offers valuable insights for the design and enhancement of LFIA assays.

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“…The presence of antigen-specific immunoglobulins G (IgG sp ) is indicative of the organism’s previous exposure to the antigen . Serological point-of-care (POC) assays are needed to effectively assess the immune response of individuals to vaccination and the infection fatality risk of pathogens for the population. , They are also pivotal for monitoring the spread of zoonotic pathogens, such as coronavirus and monkeypox virus, in wildlife and domestic animals. − …”

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confidence: 99%

Electrophoretic Assembly of Antibody–Antigen Complexes Facilitates 1000 Times Improvement in the Limit of Detection of Serological Paper-Based Assay

et al. 2023

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Serological assays detect the presence of specific antibodies in blood. There are urgent and important applications for serological point-of-care (POC) assays. However, available detection methods are either insufficiently sensitive or too complex for POC settings. Here, we demonstrate that lateral flow immunoassay (LFIA), which is arguably the simplest universal molecular detection approach, can serve as a methodological platform for highly sensitive serological POC assays if combined with a simple, fast, and inexpensive electrophoretic step. In this work, we compared such electrophoretically driven LFIA (eLFIA) with conventional LFIA for the detection of immunoglobulins G against hepatitis B and C in serum. The limit of detection of eLFIA was proven to be 1000 times lower than that of conventional LFIA and sufficiently low to support clinical serological tests. eLFIA takes less than 10 min, requires only a minor accessory powered by a small 9 V battery, and can be performed by an untrained person in the POC environment using a 3 μL specimen of finger-prick capillary blood.

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Triple Immunochromatographic System for Simultaneous Serodiagnosis of Bovine Brucellosis, Tuberculosis, and Leukemia

Cited by 5 publications

References 34 publications

Strengthening the Monitoring and Surveillance System for Bovine Tuberculosis in Post Mortem Material from Slaughtered Cattle in Eswatini: A Review

Strengthening the Monitoring and Surveillance System for Bovine Tuberculosis in Post Mortem Material from Slaughtered Cattle in Eswatini: A Review

Research on the Flow Characteristics and Reaction Mechanisms of Lateral Flow Immunoassay under Non-Uniform Flow

Electrophoretic Assembly of Antibody–Antigen Complexes Facilitates 1000 Times Improvement in the Limit of Detection of Serological Paper-Based Assay

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