Stationary phase optimized selectivity liquid chromatography: Basic possibilities of serially connected columns using the “PRISMA” principle

Nyiredy, Sz.; Szűcs, Zoltán; Szepesy, L.

doi:10.1016/j.chroma.2007.04.041

Cited by 65 publications

(28 citation statements)

References 21 publications

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“…The use of multiple solid stationary phases in stationary phase-optimized selectivity liquid chromatography (SOSLC) furnishes another approach to enhance separation [22][23][24][25]. In DMCC, separation can be enhanced by the use of multiple liquid stationary phases.…”

Section: Discussionmentioning

confidence: 99%

Dispersed Mobile-Phase Countercurrent Chromatography

Xue

2016

Separations

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Countercurrent distribution based on liquid-liquid partition is a powerful separation method with minimal incurrence of loss of solutes, but its industrial application has been limited by cumbersome shifting of immiscible solvents. Although centrifugation has been employed to facilitate equilibration between phases, process scaling-up remains difficult. In this study, a dispersed mobile-phase countercurrent chromatography (DMCC) method has been developed to adapt the countercurrent distribution principle to a continuous column chromatography format. Continuous solute exchange between two immiscible phases within a series of separation columns is achieved by mechanical dispersion of an influx of mobile phase into an upward stream of small droplets travelling through the columns filled with stationary phase. The diameter, length, and number of columns, and the number of stationary phases employed in the different columns can be varied to match the requisite scale and resolution of operation. Illustrations of DMCC were provided by examples of solute separations where the fractionated solutes could be collected either from the eluate of the series of columns, or from drainage of the stationary phases in the individual columns at the end of a chromatographic run.

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Section: Discussionmentioning

confidence: 99%

Dispersed Mobile-Phase Countercurrent Chromatography

Xue

2016

Separations

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“…It is noticed that neither retention factors nor the other chromatographic parameters (i. e. separation factors, resolution factors, column efficiencies) experienced a considerable dependency on exchange of the order of the individual columns in the tandem device. This insensitivity for the column order in the tandem column may be also regarded as an indication for the mere additivity of the chromatographic contributions which makes the process on the tandem column somehow predictable on the basis of the experiments on the individual column [11,23].…”

Section: Sequence Of Columns In the Tandem Arrangementmentioning

confidence: 99%

In‐line coupling of a reversed‐phase column to cope with limited chemoselectivity of a quinine carbamate‐based anion‐exchange type chiral stationary phase

Sardella

Lämmerhofer²,

Natalini

et al. 2008

J of Separation Science

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A chiral stationary phase based on tert-butylcarbamoyl quinine has shown remarkable enantiomer separation capability for the thyroid hormone thyroxine (T(4)) and its structural analogue triiodothyronine (T(3)) employing hydroorganic buffered mobile phases (typical RP conditions). To overcome the problem of a somehow limited chemoselectivity for the critical peak pair between adjacent L-thyroxine (L-T(4)) and D-thyroxine (D-T(4)) peaks on the chiral anion-exchanger CSP when all four compounds need to be analysed simultaneously like in impurity profiling of L-T(4 )drug products, an RP column (Gemini C18) was serially coupled with the chiral anion-exchanger column to add a hydrophobic selectivity increment and to improve thereby the critical resolution between L-T(3) and D-T(4). Various parameters such as organic modifier content, pH, buffer concentration and type, type of achiral column as well as sequence of achiral and chiral column have been investigated with individual and tandem columns. With the optimized conditions and use of the tandem column a significantly improved separation, as compared to the chiral anion-exchanger column alone, with a critical resolution as high as 3.7 and an almost equal band spacing of the four components of the test mixture could be obtained. The sequence of the columns (achiral-chiral or chiral-achiral) had no significant effect on the separation performance.

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“…This software simulates all chromatograms with all possible stationary phase compositions based on the , PRISMA' model theory which is briefly described in the following section ( Fig. 1) [15].…”

Section: Optimization Of the Stationary Phasementioning

confidence: 99%

“…The approach with serially connected stationary phases is much easily performed in a common HPLC laboratory. This method is more flexible than the previous ones because the different stationary phases can be dismantled and recombined for other separations [15].…”

Section: Introductionmentioning

confidence: 99%

Phase optimized liquid chromatography as an instrument for steroid analysis

Kuehnle¹,

Rehbein²,

Holtin³

et al. 2008

J of Separation Science

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A new method using phase optimized LC (POPLC) for the analysis of steroids is described. The retention factors and the theoretical plate numbers of different steroids were determined for four different stationary phases. Based on these values, an optimal stationary phase composition and the resultant chromatogram have been calculated by an optimization software and compared to the measured data. It is demonstrated that these predicted data show only little differences to the experimental results. Furthermore, it is shown that the overall selectivity of the optimal stationary phase composition is much better than the selectivity of any individual stationary phase.

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Stationary phase optimized selectivity liquid chromatography: Basic possibilities of serially connected columns using the “PRISMA” principle

Cited by 65 publications

References 21 publications

Dispersed Mobile-Phase Countercurrent Chromatography

Dispersed Mobile-Phase Countercurrent Chromatography

In‐line coupling of a reversed‐phase column to cope with limited chemoselectivity of a quinine carbamate‐based anion‐exchange type chiral stationary phase

Phase optimized liquid chromatography as an instrument for steroid analysis

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