Phase optimized liquid chromatography as an instrument for steroid analysis

Abstract

A new method using phase optimized LC (POPLC) for the analysis of steroids is described. The retention factors and the theoretical plate numbers of different steroids were determined for four different stationary phases. Based on these values, an optimal stationary phase composition and the resultant chromatogram have been calculated by an optimization software and compared to the measured data. It is demonstrated that these predicted data show only little differences to the experimental results. Furthermore, it is shown that the overall selectivity of the optimal stationary phase composition is much better than the selectivity of any individual stationary phase.

Time‐dependent column performance of cholesterol‐based stationary phases for HPLC by LC characterization and solid‐state NMR spectroscopy

“…Apart from the general evaluation of column performance an application to a real separation problem was performed. Depending on the SP steroid separations are often difficult to achieve; it can be time consuming and mostly require gradient conditions []. A mixture of six steroids was separated under isocratic mobile phase composition.…”

Stationary-phase optimized selectivity in supercritical fluid chromatography using a customized Phase OPtimized Liquid Chromatography kit: comparison of different prediction approaches

“…The POPLC kit was already successfully used in HPLC for the separation of mixtures containing steroids [21], explosives [22], polymer electrolyte membrane degradation products [23], and synthetic thyroid hormones and their impurities [24]. Deconinck et al [25][26][27][28] have successfully applied the POPLC method in the contexts of food-supplement analysis to screen several types of mixtures, containing analgesics, adulterated dietary supplements, PDE-5 inhibitors in food supplements and to develop herbal fingerprints of specific plants in plant food supplements.…”

Application of phase optimized liquid chromatography to oligopeptide separations

“…The POPLC approach has been used for the separation of flavonoids [2], pesticides [2], steroids [9], nitroaromatic explosives [10] and for the determination of related compounds in synthetic thyroid hormones [11], but the technique has not been applied to the analysis of oligopeptides which comprise an important class of pharmacologically active compounds. Thus, the present study was conducted in order to evaluate the potential of POPLC for the separation of small peptides.…”