Static magnetic fields affect calcium fluxes and inhibit stress‐induced apoptosis in human glioblastoma cells

Abstract

Background: Epidemiologic data revealed increased brain tumor incidence in workers exposed to magnetic fields (MFs), raising concerns about the possible link between MF exposure and cancer. However, MFs seem to be neither mutagenic nor tumorigenic. The mechanism of their tumorigenic effect has not been elucidated. Methods: To evaluate the interference of MFs with physical (heat shock, HS) and chemical (etoposide, VP16) induced apoptoses, respectively, we exposed a human glioblastoma primary culture to 6 mT static MF. We investigated cytosolic Ca 2ϩ ([Ca 2ϩ ] i ) fluxes and extent of apoptosis as key endpoints. The effect of MFs on HS-and VP16-induced apoptoses in primary glioblastoma cultures from four patients was also tested. Results: Static MFs increased the [Ca 2ϩ ] i from a basal value of 124 Ϯ 4 nM to 233 Ϯ 43 nM (P Ͻ 0.05). MF exposure dramatically reduced the extent of HS-and

Static magnetic fields affect cell size, shape, orientation, and membrane surface of human glioblastoma cells, as demonstrated by electron, optic, and atomic force microscopy

“…In this regard different models have been proposed. We have recently demonstrated that SMF increase the influx of capacitative calcium in the same cell system that we have used in this research (19). Other investigators have also found an increase of calcium fluxes in other cellular systems (30,31).…”

“…For the experimental procedures, the cells were plated on glass coverslips and, at a near sub-confluence stage, were exposed to different doses of SMF. We have chosen to investigate glioblastoma cells, as nerve cells have been demonstrated to be one of the preferred targets of magnetic fields (19).…”

Does static magnetic field-exposure induced oxidative stress and apoptosis in rat kidney and muscle? Effect of vitamin E and selenium supplementations

“…Both induced and spontaneous apoptosis have been reported to be influenced by SMF exposure. However, some reports have failed to detect any apoptotic effect for SMFs (Teodori et al 2002a(Teodori et al , 2002b(Teodori et al , 2006.…”

Perspectives of Constant Gradient Magnetic Fields Applications in Biotechnology

“…But there is a supposition that the most sensitive element to the magnetic field action a cell plasmolemma due to its bioelectric properties [18,19]. It has been reported previously that SMF of medium intensity can change plasmolemma functions in different types of cells [20][21][22][23][24][25].…”