Static magnetic fields affect cell size, shape, orientation, and membrane surface of human glioblastoma cells, as demonstrated by electron, optic, and atomic force microscopy

Abstract

Background: It is common knowledge that static magnetic fields (SMF) do not interact with living cells; thus, fewer studies of SMF compared with variable magnetic fields are carried out. However, evidence demonstrated that SMF affect cellular structures. To investigate the effect of exposure to increasing doses of SMF on cell morphology, human glioblastoma cells were exposed to SMF ranging between 80 and 3,000 G (8 and 300 mT). Methods: Cell morphology of human glioblastoma cells, derived from a primary culture, was studied by electron and optic microscopy. FITC-phalloidin staining of actin filaments was also investigated. Finally, cell surface structure changes were detected by atomic force microscopy.Results: Scanning electron microscopy demonstrated a dose-dependent cell shape modification, progressive cell detachment, loss of the long villi, and appearance of membrane roughness and blebs. FITC-phalloidin staining confirmed the villi retention and cell dimension decrease. At

The effect of low static magnetic field on osteogenic and adipogenic differentiation potential of human adipose stromal/stem cells

“…The presence of Ca 2 þ can cause changes in the actin microdomain and thus lead to interaction with integrins and adhesion molecules responsible for cell shape and geometry. By modifying Ca 2 þ concentration and orientation of actin filaments, SMF can influence the polar distribution of cellular organelles [30,45]. We have observed this phenomenon in our study.…”

“…As it was excellently described by Teodori et al [30] SMF induces reorganization of microfilaments by regulating Ca 2 þ concentration, thus regulating cell orientation. The presence of Ca 2 þ can cause changes in the actin microdomain and thus lead to interaction with integrins and adhesion molecules responsible for cell shape and geometry.…”

“…It is assumed that SMF might effect biological free radicals [27] as well as influence the concentration of Ca 2 þ [28,29] and filaments. Thus, SMF might play an important role in intracellular signaling [27,28], as well as intercellular communication [29], as well as intercellular communication, and also possibly alter cellular function [30]. Besides improving cell proliferation, SMF has an analgesic and antiinflammatory effect [31].…”

Does static magnetic field-exposure induced oxidative stress and apoptosis in rat kidney and muscle? Effect of vitamin E and selenium supplementations

“…Both induced and spontaneous apoptosis have been reported to be influenced by SMF exposure. However, some reports have failed to detect any apoptotic effect for SMFs (Teodori et al 2002a(Teodori et al , 2002b(Teodori et al , 2006.…”

Influence of static magnetic fields on nerve regeneration in vitro

“…Static magnetic fields have been tested on cultured cells (Teodori et al 2006) and animal preparations (Kelleher et al 2006) with varying measures of success. We began our studies using cultures of a chick embryo explant model of sensory neurons (Sisken et al 1984) since it is a classic system employed for studying neurobiological interactions and growth (Levi-Montalcini 1982, 2004.…”