Static and Dynamic Hypergravity Responses of Osteoblasts and Osteoclasts in Medaka Scales

Yano, Sachiko; Kitamura, Kei; Satoh, Yusuke; Nakano, Masaki; Hattori, Atsuhiko; Sekiguchi, Toshio; Ikegame, Mika; Nakashima, Hiroshi; Omori, Keisuke; Hayakawa, Kazuichi; Chiba, Atsuhiko; Sasayama, Yuichi; Ejiri, Sadakazu; Mikuni-Takagaki, Yuko; Mishima, Hiroyuki; Funahashi, Hiroomi; Sakamoto, Tatsuya; Suzuki, Nobuo

doi:10.2108/zsj.30.217

Cited by 11 publications

(9 citation statements)

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“…As a result, osteoblastic activity significantly increased under 2-to 4-G loading by both centrifugation and vibration (26), as it did in the present study. It is known that bone matrix plays an important role in the response to physical stress (6,14,24).…”

Section: Discussionsupporting

confidence: 86%

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Effects of low-intensity pulsed ultrasound on osteoclasts: Analysis with goldfish scales as a model of bone

et al. 2017

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The effects of low-intensity pulsed ultrasound (LIPUS) on osteoclastogenesis were examined using fish scales that had both osteoclasts and osteoblasts. The binding of the receptor activator of NF-κB ligand (RANKL) in osteoblasts to the receptor activator of NF-κB (RANK) in osteoclasts induced osteoclastogenesis. Therefore, we focused on RANK/RANKL signaling. After 6 h of incubation following LIPUS treatment, mRNA expression of RANKL increased significantly. Resulting from the increased RANKL mRNA level, the expression of transcription-regulating factors significantly increased after 6 h of incubation, and then the mRNA expression of functional genes was significantly up-regulated after 12 h of incubation. However, the mRNA expression of osteoprotegerin (OPG), which is known as an osteoclastogenesis inhibitory factor, also significantly increased after 6 h of incubation and tended to further increase after 12 h of incubation. At 24 h of incubation, osteoclastic functional genes' mRNA expression decreased to the level of the control. Furthermore, we performed an in vivo experiment with goldfish. Two weeks after daily LIPUS exposure, osteoclastic marker enzymes tended to decrease while osteoblastic marker enzymes were activated. The regeneration rate of the LIPUS-treated scales was significantly higher than that of the control scales. Thus, LIPUS moderately activates osteoclasts and induces bone formation.Low-intensity pulsed ultrasound (LIPUS), a noninvasive remedial measure, promotes the repair of bone fracture and distraction osteogenesis (see a review, 25). Until now, the study of LIPUS has focused on osteoblastic growth and differentiation (3,8,10). However, the details of the direct effect of

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Section: Discussionsupporting

confidence: 86%

“…We have previously analyzed scale osteoblastic and osteoclastic responses under 2-, 3-, and 4-gravity (G) loading by both centrifugation and vibration (26). As a result, osteoblastic activity significantly increased under 2-to 4-G loading by both centrifugation and vibration (26), as it did in the present study.…”

Section: Discussionsupporting

confidence: 73%

Effects of low-intensity pulsed ultrasound on osteoclasts: Analysis with goldfish scales as a model of bone

et al. 2017

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“…In osteoblasts, also, both InHg and MeHg inhibited the ALP enzyme activity at 18 and 36 hrs after incubation. It is known that the scales are calcified tissue that contains osteoclasts and osteoblasts (Bereiter-Hahn and Zylberberg, 1993;Yoshikubo et al, 2005;Suzuki et al, 2007;Suzuki et al, 2008;de Vrieze et al, 2010;Suzuki et al, 2011a;Thamamongood et al, 2012, Yano et al, 2013. Additionally, it has been reported that scales are a better potential internal calcium reservoir than body skeletons, jaws, and otoliths examined by the 45 Calabeling study of calcified tissues of goldfish and killifish (Mugiya and Watabe, 1977).…”

Section: Discussionmentioning

confidence: 99%

Effects of Inorganic Mercury and Methylmercury on Osteoclasts and Osteoblasts in the Scales of the Marine Teleost as a Model System of Bone

et al. 2014

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To evaluate the effects of inorganic mercury (InHg) and methylmercury (MeHg) on bone metabolism in a marine teleost, the activity of tartrate-resistant acid phosphatase (TRAP) and alkaline phosphatase (ALP) as indicators of such activity in osteoclasts and osteoblasts, respectively, were examined in scales of nibbler fish (Girella punctata). We found several lines of scales with nearly the same TRAP and ALP activity levels. Using these scales, we evaluated the influence of InHg and MeHg. TRAP activity in the scales treated with InHg (10 -5 and 10 -4 M) and MeHg (10 -6 to 10 -4 M) during 6 hrs of incubation decreased significantly. In contrast, ALP activity decreased after exposure to InHg (10 -5 and 10 -4 M) and MeHg (10 -6 to 10 -4 M) for 18 and 36 hrs, although its activity did not change after 6 hrs of incubation. As in enzyme activity 6 hrs after incubation, mRNA expression of TRAP (osteoclastic marker) decreased significantly with InHg and MeHg treatment, while that of collagen (osteoblastic marker) did not change significantly. At 6 hrs after incubation, the mRNA expression of metallothionein, which is a metal-binding protein in osteoblasts, was significantly increased following treatment with InHg or MeHg, suggesting that it may be involved in the protection of osteoblasts against mercury exposure up to 6 hrs after incubation. To our knowledge, this is the first report of the effects of mercury on osteoclasts and osteoblasts using marine teleost scale as a model system of bone.

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“…The transduction of mechanical signals through the canaliculi network of the teleost cellular bone is not well understood and underlying mechanisms have not yet been proven to be comparable to those in mammalian or avian bone (Fiaz et al., ). Despite being different in respect to the occurrence of osteocytes, both acellular and cellular teleost bones are capable of responding to mechanical changes and adapt accordingly (Aceto et al., ; Cardeira, Bensimon‐Brito, Pousão‐Ferreira, Cancela, & Gavaia, ; Cardeira, Mendes, Pousão‐Ferreira, Cancela, & Gavaia, ; Chatani et al., ; Fiaz et al., ; Fiaz, Léon‐Kloosterziel, et al., ; Gorman, Handrigan, Jin, Wallis, & Breden, ; Kihara, Ogata, Kawano, Kubota, & Yamaguchi, ; Kitamura et al., ; Kranenbarg, van Cleynenbreugel, Schipper, & van Leeuwen, ; Kranenbarg, Waarsing, Muller, Weinans, & van Leeuwen, ; Owen, Eynon, Woodgate, Davies, & Fox, ; Suzuki et al., ; Totland et al., ; Witten, Gil‐Martens, Hall, Huysseune, & Obach, ; Yano et al., ; Ytteborg, Torgersen, Baeverfjord, & Takle, ; Ytteborg et al., ). It is thus clear that the teleost bone holds a functional mechanosensing system, in which osteocytes (at least for the acellular bone) do not play a central role.…”

Section: Detection Of Mechanical Stimuli and Primary Cellular Responsesmentioning

confidence: 99%

“…This suggests that osteoclasts themselves can also detect changes in the biomechanical environment. In contrast to microgravity, in vitro studies of goldfish and medaka scales have shown that induced hypergravity stimulated osteoblast activity while reducing osteoclast activity (Suzuki et al., ; Yano et al., ). Similarly, hypergravity increased bone mass in developing zebrafish cranial structures (Aceto et al., ).…”

Section: Phenotypical Adaptations To Disturbed Mechanical Loadingmentioning

confidence: 99%

An overview on the teleost bone mechanophysiology

et al. 2018

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Summary Vertebrate tissues are plastic and actively adapt to the mechanical environment. Teleost bone, despite differences with its mammalian counterpart, also responds to mechanical loading, evidencing the presence of a functional mechanosensing system. Deformities such as spinal curvatures and vertebral compressions can result in internal loading disturbances. On the other hand, the external mechanical loading can be modulated by controlling gravity (i.e. inducing micro‐ or hyper‐gravity) or swimming conditions. There is an increasing interest in understanding teleost bone mechanobiology resulting from the current importance of teleosts as aquaculture resources and as biomedical models. Thus, this paper aims at reviewing relevant data that contribute to understanding fundamental questions, such as how teleost bone recognizes the biomechanical environment and how it phenotypically responds to specific mechanical changes. Particularly relevant for research purposes, technological and technical aspects of one of the most used loading‐induced systems, the swimming exercise, are also presented.

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Static and Dynamic Hypergravity Responses of Osteoblasts and Osteoclasts in Medaka Scales

Cited by 11 publications

References 40 publications

<b>Effects of low-intensity pulsed ultrasound on osteoclasts: Analysis with goldfish scales as a model of </b><b>bone </b>

<b>Effects of low-intensity pulsed ultrasound on osteoclasts: Analysis with goldfish scales as a model of </b><b>bone </b>

Effects of Inorganic Mercury and Methylmercury on Osteoclasts and Osteoblasts in the Scales of the Marine Teleost as a Model System of Bone

An overview on the teleost bone mechanophysiology

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