Stathmin 1: a protein with many tasks. New biomarker and potential target in cancer

Nemunaitis, John

doi:10.1517/14728222.2012.696101

Cited by 40 publications

(38 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…STMN1 expression in NSCLC and SCLC was not found to be an independent predictor of OS. This is in contrast with observations made in other cancers 18 and may be explained by the relative small number of SCLCs studied here (N = 36).…”

Section: Discussioncontrasting

confidence: 99%

See 1 more Smart Citation

Discovery of New Membrane-Associated Proteins Overexpressed in Small-Cell Lung Cancer

Ocak

Friedman

Chen

et al. 2014

Journal of Thoracic Oncology

View full text Add to dashboard Cite

Introduction Small-cell lung cancer (SCLC) is the most aggressive subtype of lung cancer, with no early detection strategy or targeted therapy currently available. We hypothesized that difference gel electrophoresis (DIGE) may identify membrane-associated proteins (MAPs) specific to SCLC, advance our understanding of SCLC biology, and discover new biomarkers of SCLC. Methods MAP lysates were prepared from three SCLCs, three non–small-cell lung cancers, and three immortalized normal bronchial epithelial cell lines and coanalyzed by DIGE. Subsequent protein identification was performed by mass spectrometry. Proteins were submitted to Ingenuity Pathway Analysis. Candidate biomarkers were validated by Western blotting (WB) and immunohistochemistry (IHC). Results Principal component analysis on the global DIGE data set demonstrated that the four replicates derived from each of the nine cell lines clustered closely, as did samples within the same histological group. One hundred thirty-seven proteins were differentially expressed in SCLC compared with non–small-cell lung cancer and immortalized normal bronchial epithelial cells. These proteins were overrepresented in cellular/tissue morphology networks. Dihydropyrimidinase-related protein 2, guanine nucleotide–binding protein alpha-q, laminin receptor 1, pontin, and stathmin 1 were selected as candidate biomarkers among MAPs overexpressed in SCLC. Overexpression of all candidates but RSSA in SCLC was verified by WB and/or IHC on tissue microarrays. These proteins were significantly associated with SCLC histology and survival in univariables analyses. Conclusion DIGE analysis of a membrane-associated subproteome discovered overexpression of dihydropyrimidinase-related protein 2, guanine nucleotide–binding protein alpha-q, RUVB1, and stathmin 1 in SCLC. Results were verified by WB and/or IHC in primary tumors, suggesting that investigating their functional relevance in SCLC progression is warranted. Association with survival requires further validation in larger clinical data sets.

show abstract

Section: Discussioncontrasting

confidence: 99%

“…STMN1 overexpression plays a role in tumor progression and metastasis in various cancers and contributes to chemoresistance. 18 Therefore, STMN1 appears as an attractive therapeutic target candidate. Recent integrative genome analyses showed high STMN1 gene expression levels in 15 SCLC tumors, 19 confirming our data adequately.…”

Section: Discussionmentioning

confidence: 99%

Discovery of New Membrane-Associated Proteins Overexpressed in Small-Cell Lung Cancer

Ocak

Friedman

Chen

et al. 2014

Journal of Thoracic Oncology

View full text Add to dashboard Cite

show abstract

“…[50, 51] Stathmin is over-expressed in several different cancer types. [23, 52–55] High levels of stathmin have been associated with poor prognosis in multiple cancer types and resistance to drugs stabilizing microtubules, such as taxane.…”

Section: Discussionmentioning

confidence: 99%

Proteomic Signatures of Thymomas

et al. 2016

View full text Add to dashboard Cite

Based on the histological features and outcome, the current WHO classification separates thymomas into A, AB, B1, B2 and B3 subtypes. It is hypothesized that the type A thymomas are derived from the thymic medulla while the type B thymomas are derived from the cortex. Due to occasional histological overlap between the tumor subtypes creating difficulties in their separation, the aim of this study was to provide their proteomic characterization and identify potential immunohistochemical markers aiding in tissue diagnosis. Pair-wise comparison of neoplastic and normal thymus by liquid chromatography tandem mass spectrometry (LC-MS/MS) of formalin fixed paraffin embedded tissue revealed 61 proteins differentially expressed in thymomas compared to normal tissue. Hierarchical clustering showed distinct segregation of subtypes AB, B1 and B2 from that of A and B3. Most notably, desmoyokin, a protein that is encoded by the AHNAK gene, was associated with type A thymomas and medulla of normal thymus, by LC-MS/MS and immunohistochemistry. In this global proteomic characterization of the thymoma, several proteins unique to different thymic compartments and thymoma subtypes were identified. Among differentially expressed proteins, desmoyokin is a marker specific for thymic medulla and is potentially promising immunohistochemical marker in separation of type A and B3 thymomas.

show abstract

“…These functions include mitosis, intracellular transport, polarity, and cell motility [34,35]. Dynamics of microtubule function can be best described as an alternating pattern of stabilization and destabilization.…”

Section: Discussionmentioning

confidence: 99%

Reduced STMN1 expression induced by RNA interference inhibits the bioactivity of pancreatic cancer cell line Panc-1

Li¹,

Gh²,

Fj³

et al. 2014

neo

View full text Add to dashboard Cite

Increased expression of STMN1 has been observed in many tumor forms, but its expression and potential biological role in pancreatic cancer is still unknown. In this study, we demonstrated that STMN1 was expressed to a large extent in pancreatic cancer tissues and cell lines as compared to normal pancreatic tissues. Suppression of STMN1 expression via transfection with STMN1-specific siRNA could not only significantly inhibit the proliferation, migration and invasion ability of Panc-1 cells, but also enhance the apoptosis of Panc-1 cells. In addition, downregulation of STMN1 obviously enhanced the acetylation level of α-tubulin. All these results indicated that STMN1 plays an important role in pancreatic cancer development, and might serve as a potential therapeutic target for pancreatic cancer.

show abstract

Stathmin 1: a protein with many tasks. New biomarker and potential target in cancer

Cited by 40 publications

References 38 publications

Discovery of New Membrane-Associated Proteins Overexpressed in Small-Cell Lung Cancer

Discovery of New Membrane-Associated Proteins Overexpressed in Small-Cell Lung Cancer

Proteomic Signatures of Thymomas

Reduced STMN1 expression induced by RNA interference inhibits the bioactivity of pancreatic cancer cell line Panc-1

Contact Info

Product

Resources

About