State-of-the-Art of Serum Testosterone Measurement by Isotope Dilution–Liquid Chromatography– Tandem Mass Spectrometry

Thienpont, Linda M.; Uytfanghe, Katleen Van; Blincko, Stuart; Ramsay, Carol; Xie, Hui; Doss, Robert C.; Keevil, Brian; Owen, Laura; Rockwood, Alan L.; Kushnir, Mark M.; Chun, Kelly Y.; Chandler, Donald Walt; Field, H P; Sluss, Patrick M.

doi:10.1373/clinchem.2008.105841

Cited by 131 publications

(67 citation statements)

References 19 publications

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“…Recent comparison of the in-house method with a dilution liquid (ID)-GC/MS method using 20 human serum samples of the Dutch EQAS gave the following correlation: RIAZ1.13! (ID-GC/MS)C0.33 nmol/l (tested range was 0.8-33 nmol/l; 95% confidence limit of the slope: 1.04-1.20 and of the intercept: K1.04 to 0.91) (23). Furthermore, the following measurements were performed on fresh serum for women with POI and PCOS: estradiol (E 2 ), SHBG, DHEA, DHEAS, and AD.…”

Section: Measurementsmentioning

confidence: 99%

Assessment of androgen concentration in women: liquid chromatography–tandem mass spectrometry and extraction RIA show comparable results

Janse

Eijkemans

Goverde

et al. 2011

European Journal of Endocrinology

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Objective: The measurement of serum testosterone in women is challenging due to lack of trueness, precision, and sensitivity of various available testosterone assays. Accurate assessment of testosterone in women is crucial especially in conditions associated with alleged over-or under-production of testosterone, such as in polycystic ovary syndrome (PCOS) or primary ovarian insufficiency (POI). The aim of this study was to measure and compare androgen concentrations in women with PCOS, POI, and female controls and to evaluate the performance of extraction RIA and liquid chromatographytandem mass spectrometry (LC-MS/MS) in these women. Design: Cross-sectional study. Methods: Carefully phenotyped women with POI (nZ208) or PCOS (nZ200) and 45 healthy, regularly cyclic female controls were included. Method comparison analyses were performed for total testosterone, androstenedione (AD), and DHEA, as measured by LC-MS/MS and extraction RIA. Results: All androgen levels were significantly elevated in women with PCOS compared with POI patients (P!0.05) and controls (P!0.05). Women with POI presented with similar androgen concentrations as controls, except for AD. Compared with measurements by extraction RIA, testosterone, DHEA, and AD concentrations measured by LC-MS/MS were systematically lower. However, using extraction RIA and LC-MS/MS, testosterone, DHEA, and AD measurements were shown to have good agreement as assessed by Bland-Altman analysis and intraclass correlation coefficient: 0.95 (95% confidence interval 0. 94-0.91), 0.83 (0.79-0.86), and 0.96 (0.95-0.97) respectively. Conclusions: LC-MS/MS, compared with a labor-intensive extraction RIA, shows good precision, sensitivity, and high accuracy for measuring female testosterone, DHEA, and AD concentrations under various clinical conditions. LC-MS/MS, therefore, represents a convenient and reliable assay for both clinical and research purposes, where androgen measurement in women is required. European Journal of Endocrinology 165 925-933

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Section: Measurementsmentioning

confidence: 99%

Assessment of androgen concentration in women: liquid chromatography–tandem mass spectrometry and extraction RIA show comparable results

Janse

Eijkemans

Goverde

et al. 2011

European Journal of Endocrinology

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“…A chromatogram of Te extracted from the serum of a healthy woman is shown in Figure 6, and the method's performance characteristics are listed in Table 2. Considering the analytical sensitivity and small volume of sample used (100 mL), this method is one of the most sensitive of the published LC-MS/MS methods for the measurement of Te (Cawood et al, 2005;Vicente et al, 2006;Borrey et al, 2007;Thienpont et al, 2008). To address the issue of poor performance of commercial IAs for Te, it was suggested to use the isotope-dilution LC-MS/MS method for the routine measurement of Te in samples from women and children (Rosner et al, 2007).…”

Section: Androgensmentioning

confidence: 99%

“…To address the issue of poor performance of commercial IAs for Te, it was suggested to use the isotope-dilution LC-MS/MS method for the routine measurement of Te in samples from women and children (Rosner et al, 2007). Currently, a majority of the testing for Te in samples from women and children in commercial clinical laboratories in developed countries is performed with LC-MS/MS methods (Thienpont et al, 2008). To enable the use of this method in clinical diagnostic practice, reference intervals were established using LC-MS/MS method with samples from healthy volunteers for total Te (free and bound to carrier proteins), bioavailable Te (free and bound to albumin), and free Te in the serum of women, men, and children (Kushnir et al, 2006a).…”

Section: Androgensmentioning

confidence: 99%

Liquid chromatography-tandem mass spectrometry applications in endocrinology

Kushnir

Rockwood

Bergquist

2009

Mass Spectrom. Rev.

138

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Liquid chromatography-tandem mass spectrometry (LC-MS/ MS) has been recognized as a primary methodology for the accurate analysis of endogenous steroid hormones in biological samples. This review focuses on the use of LC-MS/MS in clinical laboratories to assist with the diagnosis of diverse groups of endocrine and metabolic diseases. Described analytical methods use on-line and off-line sample preparation and analytical derivatization to enhance analytical sensitivity, specificity, and clinical utility. Advantages of LC-MS/MS as an analytical technique include high specificity, possibility to simultaneously measure multiple analytes, and the ability to assess the specificity of the analysis in every sample. All described analytical methods were extensively validated, utilized in routine diagnostic practice, and were applied in a number of clinical and epidemiological studies, including a study of the steroidogenesis in ovarian follicles. #

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“…Standardization is crucial to improve patient care, otherwise the proliferation of procedures using the method could impair the LC-MS/MS, which is a method with good precision. This conclusion is true for all methods used for the measurement of testosterone 33 …”

Section: (A)mentioning

confidence: 69%