State-of-the-art mass spectrometry imaging applications in biomedical research

Krestensen, Kasper K.; Heeren, Ron M. A.; Balluff, Benjamin

doi:10.1039/d3an01495a

Cited by 2 publications

(1 citation statement)

References 157 publications

(211 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Each peak represents an image channel that can be mapped across the entire tissue section. There are many sample preparation approaches dependent on the study hypothesis and configurations of mass spectrometry instrumentation [154–156]. This review reports on detection of ECM by MSI, which we term ECM‐MSI.…”

Section: Mass Spectrometry Imaging Of Ecmmentioning

confidence: 99%

Molecular analysis of the extracellular microenvironment: from form to function

Macdonald,

Mehta,

Drake

et al. 2024

FEBS Letters

View full text Add to dashboard Cite

The extracellular matrix (ECM) proteome represents an important component of the tissue microenvironment that controls chemical flux and induces cell signaling through encoded structure. The analysis of the ECM represents an analytical challenge through high levels of post‐translational modifications, protease‐resistant structures, and crosslinked, insoluble proteins. This review provides a comprehensive overview of the analytical challenges involved in addressing the complexities of spatially profiling the extracellular matrix proteome. A synopsis of the process of synthesizing the ECM structure, detailing inherent chemical complexity, is included to present the scope of the analytical challenge. Current chromatographic and spatial techniques addressing these challenges are detailed. Capabilities for multimodal multiplexing with cellular populations are discussed with a perspective on developing a holistic view of disease processes that includes both the cellular and extracellular microenvironment.

show abstract

Section: Mass Spectrometry Imaging Of Ecmmentioning

confidence: 99%

Molecular analysis of the extracellular microenvironment: from form to function

Macdonald,

Mehta,

Drake

et al. 2024

FEBS Letters

View full text Add to dashboard Cite

show abstract

Matrix‐assisted laser desorption/ionization matrix incorporation evaluation algorithm for improved peak coverage and signal‐to‐noise ratio in mass spectrometry imaging

Kuzin,

Sobolev,

Eliferov

et al. 2024

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

RationaleDespite decades of implementation, the selection of optimal sample preparation conditions for matrix‐assisted laser desorption/ionization (MALDI) imaging is still ambiguous due to the lack of a universal and comprehensive evaluation methodology. Thus, numerous experiments with different matrix application conditions accompany a translation of the method to novel sample types and matrices.MethodsMouse brain tissues were covered with 9‐aminoacridine through sublimation, followed by recrystallization in vapors of 5% (v/v) methanol solution in water. The samples were analyzed by MALDI time‐of‐flight mass spectrometry, and the efficiency of lipid and small‐molecule ionization was evaluated with different metrics.ResultsWe first investigate the dependency of matrix density and recrystallization conditions on the thickness of an analyte‐empty matrix layer to roughly evaluate the laser shot number required to obtain an intense signal with minimal noise. Then, we introduce metrics for the analysis of small imaging datasets (small sample regions) of model samples based on median quantity of peaks in spectra (medQP) and weighted median signal‐to‐noise ratio (wmSNR). The evaluation of small regions and taking median values for metrics help overcome the sample heterogeneity and allow for the simultaneous comparison of different acquisition parameters.ConclusionsHere, we propose a methodology based on gradual laser ablation of small regions of sample and further implementation of weighted signal‐to‐noise ratio to assess various matrix application conditions. The proposed approach helps reduce the number of test samples required to determine optimal sample preparation conditions and improve the overall quality of images.

show abstract

State-of-the-art mass spectrometry imaging applications in biomedical research

Abstract: Mass spectrometry imaging (MSI) has advanced from a niche technique to a widely applied spatial biology tool. This review highlights state-of-the-art MSI application in emerging and the six largest biomedical fields according to a set of criteria.

Cited by 2 publications

References 157 publications

Molecular analysis of the extracellular microenvironment: from form to function

Molecular analysis of the extracellular microenvironment: from form to function

Matrix‐assisted laser desorption/ionization matrix incorporation evaluation algorithm for improved peak coverage and signal‐to‐noise ratio in mass spectrometry imaging

Contact Info

Product

Resources

About