State of the art in sheep-goat embryo transfer

Cognie, Y.

doi:10.1016/s0093-691x(98)00235-0

Cited by 163 publications

(98 citation statements)

References 79 publications

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“…Embryos produced by this procedure were competent to generate pregnancy, thus validating a simplified superovulation protocol when using FSH, which traditionally has been given 6-8 times every 12 h to effectively stimulate superovulation (Cognie 1999). Such a traditional procedure can cause animal stress and is also time-consuming and labourintensive.…”

Section: Discussionmentioning

confidence: 90%

A simplified superovulation protocol using split-single administration of Folltropin®-V in hyaluronan: application to purebred sheep

Panyaboriban¹,

Suwimonteerabutr²,

Swangchan-Uthai³

et al. 2018

Vet. Med. - Czech

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ABSTRACT:Superovulation is an important step in assisted reproductive technology. Due to its short half-life, follicle stimulating hormone is usually given twice daily to ewes for three to five days, which is both time-and labour-intensive. However, dissolving follicle stimulating hormone in degradable polymers to delay absorbtion has been effective in ruminants. Experiment 1 was performed to compare a split-single follicle stimulating hormone dissolved in hyaluronan (S group; 150 mg follicle stimulating hormone on the first day and 30 mg 48 h later; n = 21) and six decreasing doses of follicle stimulating hormone (M group; 50, 50, 30, 30, 10 and 10 mg; n = 22) at 12-h intervals. Ovarian responses and numbers of recovered ova/embryos did not differ significantly between groups. However, there tended to be more Grade 1 and 2 embryos in S vs M groups (mean ± SEM, 5.1 ± 4.9 vs 2.9 ± 2.9, respectively; P = 0.08). Experiment 2 tested the effectiveness of a simplified split-single follicle stimulating hormone in purebred sheep on a commercial farm. The numbers of recovered good-grade embryos (day 2) were 4.8 ± 5.0 and 4.0 ± 2.5 per donors in Corriedale and Bond sheep breeds, respectively. We conclude that this modified technique for ewe superovulation improved animal welfare, reduced animal handling and labour and yielded results similar to or better than conventional twice-daily follicle stimulating hormone treatments.

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Section: Discussionmentioning

confidence: 90%

A simplified superovulation protocol using split-single administration of Folltropin®-V in hyaluronan: application to purebred sheep

Panyaboriban¹,

Suwimonteerabutr²,

Swangchan-Uthai³

et al. 2018

Vet. Med. - Czech

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“…The reduction in superovulatory response can also be ascribed to the formation of post operative adhesions following repeated surgical collection of the embryos. This factor has also been shown to reduce ovulation rate and the number of embryos recovered (Al-Kamali et al, 1985;Cognie, 1999). Table 1 The mean (± s.e.)…”

Section: Resultsmentioning

confidence: 99%

Can repeated superovulation and embryo recovery in Boer goats limit donor participation in a MOET programme?

2010

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________________________________________________________________________________ AbstractThis study evaluated the effect of repeated superovulation and embryo recovery in 15 Boer goat does. Does were synchronised for oestrus using CIDR's for 17 days and superovulated with pFSH during the natural breeding season (autumn). Cervical inseminations with fresh undiluted semen were performed 36 h and 48 h following CIDR removal and the embryos surgically flushed six days after the second AI. Does superovulated for the first time recorded a shorter mean (± s.e.) induced duration of oestrus (20.8 ± 1.0 h), when compared to those repeatedly superovulated (30.4 ± 6.7 h). The mean (±s.e.) number of structures and embryos recovered were significantly lower in does treated repeatedly (6.0 ± 1.7 and 3.8 ± 1.7) than does superovulated for the first time (12.9 ± 0.5 and 11.7 ± 0.5), respectively. The mean (±s.e.) number of unfertilised ova per donor was significantly higher in repeatedly superovulated does (5.5 ± 1.6), compared to does superovulated for the first time (0.1 ± 0.1). The fertilisation rate and the number of transferable embryos were significantly lower in does treated repeatedly, compared to does superovulated for the first time. These results indicate that the number of times that a Boer goat doe can be utilised as the embryo donor may be limited to three times. _______________________________________________________________________

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“…Superstimulation of ovaries is more successful in the absence of large follicles (BREBION et al, 1992;COGNIÉ, 1999), the removal creates an ideal environment for ovarian Denadai et al response to these treatments. In this study, follicular ablation via LOPU proved effective in controlling the ovarian follicular population following removal of the largest follicles, including those with aged oocytes and large diameters.…”

Section: Discussionmentioning

confidence: 99%

“…The development of techniques for ovarian superstimulation in ewes is limited by the wide variation in responses to gonadotropin stimulation, among other causes, the presence of dominant follicles at the initiation of treatment is a major interfering factor (TERVIT et al, 1992;BALDASSARRE et al, 1994), since response to FSH is inversely proportional to the presence of follicles larger than 5mm (BREBION & COGNIÉ, 1989;COGNIÉ, 1999).…”

Section: Introductionmentioning

confidence: 99%

Pattern of follicular development in sheep subjected to ovarian superstimulation after follicular ablation by laparoscopic ovum pick-up

et al. 2017

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The aim of this study was to determine the pattern of follicular development in ewes following ablation of all follicles associated with ovarian superstimulatory treatment. After confirmation of reproductive cyclicity using ultrasonography and progesterone levels, five adult ewes of undetermined breed received a vaginal pessary containing 60mg of medroxyprogesterone, maintained for 10 days, with the application of 100μg cloprostenol on the fourth day. On the tenth day, 300UI equine chorionic gonadotropin (eCG) was applied together with 80mg FSH, and all visible follicles were aspirated by laparoscopic ovum pick-up (LOPU). Following LOPU, ovarian structures were assessed using sonography every 8 hours for a period of 64 hours, and the number and diameter of small (<2.5mm), medium-sized (2.5-4.5mm), and large (>4.5mm) follicles were recorded. The average diameter of small follicles reduced from 5.6±1.5mm initially to 0.8±1.3mm by 56 hours. Population size of the medium-sized follicles during the observation period followed a parabolic distribution wherein the theoretical maximum size emerged at 34.6 hours with no difference at (P>0.05) 24 (4.6±1.5) and 48 hours (4.8±2.3). It was concluded that in ewes subjected to ovarian superstimulation immediately following LOPU, follicular development is characterized by a new wave of follicle growth with a predominance of medium-sized follicles (2.5-4.5mm) between 24 and 48 hours, and a theoretical population maximum occured at 34.6 hours.

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State of the art in sheep-goat embryo transfer

Cited by 163 publications

References 79 publications

A simplified superovulation protocol using split-single administration of Folltropin®-V in hyaluronan: application to purebred sheep

A simplified superovulation protocol using split-single administration of Folltropin®-V in hyaluronan: application to purebred sheep

Can repeated superovulation and embryo recovery in Boer goats limit donor participation in a MOET programme?

Pattern of follicular development in sheep subjected to ovarian superstimulation after follicular ablation by laparoscopic ovum pick-up

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