Can repeated superovulation and embryo recovery in Boer goats limit donor participation in a MOET programme?

Lehloenya, K.C.; Greyling, J.P.C.; Grobler, S.

doi:10.4314/sajas.v39i1.61216

Cited by 5 publications

(6 citation statements)

References 22 publications

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“…Superovulation in goats can generally be performed utilising FSH or eCG gonadotrophins. The FSH protocol most commonly used involves oestrous synchronisation with the aid of progestagens for 9-17, days followed by the administration of FSH for 6-8 dosages, beginning approximately 48 h before progestagen removal (Gonzalez-Bulnes et al, 2003, Goel and Agrawal, 2005, Lehloenya et al, 2008, Lehloenya et al, 2009and Lehloenya and Greyling, 2010. On the other hand, eCG is usually administered as a single injection, due to its long halflife, approximately 48 h before progestagen removal (Espinosa-Marquez et al, 2004 andHoltz, 2005).…”

Section: Introductionmentioning

confidence: 99%

Preliminary results evaluating a simplified superovulation protocol in Boer goats

Lehloenya

2013

Small Ruminant Research

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This study was conducted to evaluate the use of one shot follicle stimulating hormone (FSH) plus equine chorionic gonadotrophin (eCG) (simplified) superovulation protocol in mature Boer goat does. Eighteen does used were allocated into two groups, during the natural breeding season (autumn). The two group were Group 1: the control (n = 9); multiple FSH protocol and Group 2: treatment group (n = 9); one shot FSH plus eCG protocol. All does were synchronised for oestrus, using controlled internal drug release dispensers (CIDR's) for 9 days. Does in the multiple FSH protocol group were superovulated with 200 μg pFSH/doe, administered i.m. in 7 dosages at 12 h intervals, starting 48 h prior to CIDR removal. In the treatment group (one shot FSH plus eCG protocol), the superovulation treatment was administered once as 200 μg FSH plus 350 IU eCG, 48 h prior to CIDR removal. All does were then observed for oestrous behaviour twice daily, at 12 h intervals following CIDR removal for a period of 72 h. Cervical inseminations (with 0.1 ml fresh undiluted Boer goat semen) were performed 36 h and 48 h following CIDR removal. Embryos were then surgically flushed on day 6 following the second artificial insemination. The time interval from CIDR removal to onset of oestrus was not affected by treatment. The duration of the oestrous period however was significantly longer in the one shot group, compared to the multiple FSH protocol. Does from the one shot FSH plus eCG protocol recorded a significantly (p < 0.05) lower mean number of CL's, structures (unfertilised ova plus embryos) and (p < 0.01) embryos recovered. A significantly (p < 0.01) higher number of unfertilised ova were observed from the one shot FSH plus eCG group (4.06 ± 0.9), compared to the multiple FSH group (0.24 ± 0.1). However, the number of degenerated embryos was not affected by treatment. The total number of transferable embryos was significantly lower in the one shot FSH plus eCG protocol (0.22 ± 0.2), compared to the multiple FSH protocol (9.56 ± 1.9). The size of follicles was not affected by treatment, however the total number of follicles at CIDR removal was significantly lower in the one shot FSH plus eCG group (11.67 ± 0.7), compared to the multiple FSH group (14.83 ± 0.7). AI might have been performed at the wrong time, because of the duration of the oestrous period being longer in Group 2. The multiple FSH superovulation protocol overall seemed to be more efficient in the Boer goat. More research is needed on the doses of gonadotrophins administered and the time of AI, in order for the simplified protocol (Group 2) to be effective.

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Section: Introductionmentioning

confidence: 99%

Preliminary results evaluating a simplified superovulation protocol in Boer goats

Lehloenya

2013

Small Ruminant Research

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“…These results imply that the limited potential of the animal for repeated embryo recovery could be due to the formation of post-operative adhesions in the reproductive tract [ 35 , 163 ]. Due to invasive embryo procedures, the ovarian response in goats also decreased when treated with gonadotropins [ 94 , 164 ]. The close interval between the two successive treatments may reduce the efficiency of repeated superovulation [ 15 ].…”

Section: Successive Embryo Production Through Repeated Superovulationmentioning

confidence: 99%

Towards Improving the Outcomes of Multiple Ovulation and Embryo Transfer in Sheep, with Particular Focus on Donor Superovulation

Khan

Jamal

et al. 2022

Veterinary Sciences

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Considerable improvements in sheep multiple ovulation and embryo transfer (MOET)protocols have been made; however, unlike for cattle, MOET is poorly developed in sheep, and thus has not been broadly applicable as a routine procedure. The tightly folded nature of the ewe cervix, the inconsistent ovarian response to various superovulatory treatments, and the requirement of labor to handle animals, particularly during large-scale production, has limited the implementation of successful MOET in sheep. Moreover, several extrinsic factors (e.g., sources, the purity of gonadotrophins and their administration) and intrinsic factors (e.g., breed, age, nutrition, reproductive status) severely limit the practicability of MOET in sheep and other domestic animals. In this review, we summarize the effects of different superovulatory protocols, and their respective ovarian responses, in terms of ovulation rate, and embryo recovery and transfer. Furthermore, various strategies, such as inhibin immunization, conventional superovulation protocols, and melatonin implants for improving the ovarian response, are discussed in detail. Other reproductive techniques and their relative advantages and disadvantages, such as artificial insemination (AI), and donor embryo recovery and transfer to the recipient through different procedures, which must be taken into consideration for achieving satisfactory results during any MOET program in sheep, are also summarized in this article.

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“…Different synchronization methods have been used in cycling does, as well as in seasonally anestrous does in order to induce or synchronize estrous (Amoah and Gelaye 1990;Amoah et al 1996). The fertilization rate and the number of transferable embryos are significantly lower in does treated repeatedly, compared to does superovulated for the first time (Lehloenya et al 2009). Progestagen administration is common, especially in seasonally anestrous small ruminants (Kusina et al 2000), and is used with or without accompanying treatments such as gonadotropin or prostaglandin analogues (Gordon 1997;Zarkawi et al 1999).…”

Section: Introductionmentioning

confidence: 96%

Ovarian response and embryo yield of Angora and Kilis goats given the day 0 protocol for superovulation in the non-breeding season

Taşdemir¹,

Ağaoğlu

Kaymaz

et al. 2011

Trop Anim Health Prod

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The aim of this study was to compare ovarian response and embryo yield of Day 0 protocol in Angora goats (AG) and indigenous Kilis goats (KG) in the non-breeding season. A total of 16 Angora goats (AG group) and 11 Kilis goats (KG group) were used in this study. In the synchronization process, after controlled internal drug release withdrawal, when estrus signs were observed, natural mating was performed. Ovarian response was determined by synchronized laparotomy 6 days after natural mating, and number of corpora lutea (CL) was recorded. Embryos were collected and morphologically evaluated by stereomicroscope. Synchronization rates did not differ between AG (88%, 14/16) and KG group (91%, 10/11). In AG and KG groups, the proportion of CL on the right (44% and 53%, respectively) and left (56% and 47%, respectively) ovaries were similar. The CL number per animal did not differ significantly between the two breeds and was determined as 4.4 ± 0.90 in AG group and 6.4 ± 1.44 in KG group. Transferable embryo yields were significantly higher in AG group (31/42, 74%) compared to KG group (16/46, 35%) in the non-breeding season (P < 0.01). In conclusion, it is suggested that the day 0 protocol can be used for goat superovulation in the non-breeding season; however, transferable embryo yields are affected by the breed.

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Can repeated superovulation and embryo recovery in Boer goats limit donor participation in a MOET programme?

Cited by 5 publications

References 22 publications

Preliminary results evaluating a simplified superovulation protocol in Boer goats

Preliminary results evaluating a simplified superovulation protocol in Boer goats

Towards Improving the Outcomes of Multiple Ovulation and Embryo Transfer in Sheep, with Particular Focus on Donor Superovulation

Ovarian response and embryo yield of Angora and Kilis goats given the day 0 protocol for superovulation in the non-breeding season

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