State of the art in in-vitro oocyte maturation

Abstract

In-vitro maturation treatment can now be offered as a successful option to infertile women with polycystic ovaries or polycystic ovary syndrome. It is possible to combine natural cycle in-vitro fertilization with immature oocyte retrieval followed by in-vitro maturation, and thus offer women with various causes of infertility reasonable pregnancy and implantation rates without recourse to ovarian stimulation. Further research remains to be done to address the mechanism of oocyte maturation in order to refine culture conditions and improve the implantation rate of oocytes matured in vitro.

Fertilization, embryo development, and clinical outcome of immature oocytes obtained from natural cycle in vitro fertilization

“…In this study, therefore, we collected data from patients who had the same protocol of natural cycle IVF/M as previously reported [8,10] and analyzed the embryological and clinical results of natural cycle IVF for women who had normal ovaries. We compared embryos produced from oocytes matured in vivo and in vitro and found that sibling immature oocytes could mature in vitro, fertilize and some of them could develop to good quality embryos similar to the animal model that was reported by Chian [16].…”

“…Recently, the concept of "natural cycle IVF combined with IVM (IVF/M)" has been proposed, which suggests that natural cycle IVF might be combined with immature oocyte retrieval and IVM [7][8][9][10][11]. In addition to the embryo produced by the mature oocyte from the DF, embryos produced by the IVM oocytes will also be available, therefore possibly increasing the chances of pregnancy in a natural cycle.…”

“…Administrating hCG when the DF reaches 14 mm diameter Capsule Although immature oocytes from natural cycle IVF can fertilize normally and can be used to increase the number of embryos available for transfer, the embryos derived from the immature oocytes in natural cycles IVF have a poorer reproductive potential. [8] or more recently 12-14 mm [9][10][11] to obtain mature oocytes and viable sibling immature oocytes has been recommended. However, evidence is lacking on whether the immature oocytes retrieved after the follicle gains dominance (> 12 mm) in a natural cycle actually contribute to the pregnancy rate.…”

In-vitro maturation of human oocytes: before or after vitrification?

“…The development of an effective cryopreservation program for immature oocytes may have a major impact on in vitro fertilization (IVF) clinical practice, especially to preserve the fertility of women who will be treated with cytotoxic drugs [1,2] or for women with polycystic ovarian syndrome (PCOS) at risk of ovarian hyper-stimulation syndrome (OHSS) [3][4][5]. Moreover, for cancer patients, transplantation of cryopreserved ovarian tissue is not always conceivable because of the risk of retransmission of the disease due to the presence of neoplasic cells in the tissue.…”

Pregnancies and births resulting from in vitro matured oocytes fertilized with testicular spermatozoa

“…Recently it has been demonstrated that immature oocyte retrieval followed by in vitro maturation (IVM) is a successful treatment especially for women with polycystic ovary syndrome (PCOS) (1). The major benefits of IVM treatment include avoiding the side effects of hormone stimulation, reducing direct and indirect costs and simplifying the treatment.…”