State of research in the field of the creation of plant vaccines for veterinary use

Permyakova, N. V.; Уварова, Е. А.; Дейнеко, Е. В.

doi:10.1134/s1021443715010100

Cited by 4 publications

(1 citation statement)

References 90 publications

(87 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The ability to enhance the transcription of heterologous genes and, potentially, the translation of their mRNA due to synthetic CG-rich sequence is biotechnologically important because plant cells can be more efficient for the synthesis of heterologous proteins, including pharmaceuticals, than bacterial cells. In particular, it can be useful for the proteins that require posttranslational modification [35]. Using regulatory codes of plant origin as modulators of transcription and/or translation may also be useful in experimental models of transgenic plants.…”

Section: Discussionmentioning

confidence: 99%

Efficient expression of a heterologous gene in plants depends on the nucleotide composition of mRNA’s 5'-region

Tyurin

Кабардаева

Gra

et al. 2016

Russ J Plant Physiol

View full text Add to dashboard Cite

The contribution of nucleotide composition of mRNA 5'-region to the efficiency of expression at transcriptional and translational levels was studied in transgenic tobacco plants (Nicotiana tabacum L., cultivar Petit Havana) using a thermostable lichenase reporter gene. Synthetic sequence that contains CG-rich motifs, typical for 5'-region of plant genes, identified in silico, was constructed. Transgenic plant lines of N. tabacum were obtained; they contain thermostable lichenase reporter gene that is under control of the constitutive 35S RNA CaMV promoter and additional regulatory element: synthetic CG-rich sequence, which functions as the 5'-UTR (untranslated region) of the reporter gene mRNA or as 5'-region of the hybrid gene coding sequence, wherein the synthetic sequence is fused with the sequence of the reporter gene in its reading frame. Results of the comparative analysis of mRNA and protein levels in the obtained lines of transgenic plants showed that the synthetic CG-rich sequence significantly increases the level of transcription of the reporter gene and appear to have no negative effect on the efficiency of reporter mRNA translation, which may be due to the peculiarities of its nucleotide composition and structure, namely, due to the presence of motifs that are specific for 5'-regions of plant genes, as well as due to the properties of the secondary structure-the absence of hairpin structures with a high energy of formation. It was experimentally confirmed for the first time that the 5'-region of the genes with a high content of CpG dinucleotides can help to increase the transcription level of genes in plants.

show abstract

Section: Discussionmentioning

confidence: 99%

Efficient expression of a heterologous gene in plants depends on the nucleotide composition of mRNA’s 5'-region

Tyurin

Кабардаева

Gra

et al. 2016

Russ J Plant Physiol

View full text Add to dashboard Cite

show abstract

Abnormal development of floral meristem triggers defective morphogenesis of generative system in transgenic tomatoes

et al. 2018

View full text Add to dashboard Cite

Parthenocarpy and fruit malformations are common among independent transgenic tomato lines, expressing genes encoding different pathogenesis-related (PR) protein and antimicrobal peptides. Abnormal phenotype developed independently of the expression and type of target genes, but distinctive features during flower and fruit development were detected in each transgenic line. We analyzed the morphology, anatomy, and cytoembryology of abnormal flowers and fruits from these transgenic tomato lines and compared them with flowers and fruits of wild tomatoes, line YaLF used for transformation, and transgenic plants with normal phenotype. We confirmed that the main cause of abnormal flower and fruit development was the alterations of determinate growth of generative meristem. These alterations triggered different types of anomalous growth, affecting the number of growing ectopic shoots and formation of new flowers. Investigation of the ovule ontogenesis did not show anomalies in embryo sac development, but fertilization did not occur and embryo sac degenerated. Nevertheless, the ovule continued to differentiate due to proliferation of endothelium cells. The latter substituted embryo sac and formed pseudoembryonic tissue. This process imitated embryogenesis and stimulated ovary growth, leading to the development of parthenocarpic fruit. We demonstrated that failed fertilization occurred due to defective male gametophyte formation, which was manifested in blocked division of the nucleus in the microspore and arrest of vegetative and generative cell formation. Maturing pollen grains were overgrown microspores, not competent for fertilization but capable to induce proliferation of endothelium and development of parthenocarpic ovary. Thus, our study provided new data on the structural transformations of reproductive organs during development of parthenocarpic fruits in transgenic tomato.

show abstract

Production of recombinant proteins in plant cells

Gerasimova

Smirnova

Кочетов

et al. 2016

Russ J Plant Physiol

View full text Add to dashboard Cite

State of research in the field of the creation of plant vaccines for veterinary use

Cited by 4 publications

References 90 publications

Efficient expression of a heterologous gene in plants depends on the nucleotide composition of mRNA’s 5'-region

Efficient expression of a heterologous gene in plants depends on the nucleotide composition of mRNA’s 5'-region

Abnormal development of floral meristem triggers defective morphogenesis of generative system in transgenic tomatoes

Production of recombinant proteins in plant cells

Contact Info

Product

Resources

About