State of QTL Detection and Marker‐Assisted Selection in Wheat Improvement

Somers, Daryl J.; Humphreys, Gavin

doi:10.1002/9780813818832.ch14

Cited by 3 publications

(3 citation statements)

References 86 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several studies compared DH and RIL populations for linkage map construction and QTL analysis. Somers and Humphreys ( 2009 ) found that wheat RILs had nearly twice the number of breakpoints per chromosome compared to DHs. This is consistent with theoretical expectation as RILs pass through more meiotic events in development and should have greater recombination creating more breakpoints along the chromosomes.…”

Section: Rilsmentioning

confidence: 99%

Accelerated Generation of Selfed Pure Line Plants for Gene Identification and Crop Breeding

et al. 2017

View full text Add to dashboard Cite

Production of pure lines is an important step in biological studies and breeding of many crop plants. The major types of pure lines for biological studies and breeding include doubled haploid (DH) lines, recombinant inbred lines (RILs), and near isogenic lines (NILs). DH lines can be produced through microspore and megaspore culture followed by chromosome doubling while RILs and NILs can be produced through introgressions or repeated selfing of hybrids. DH approach was developed as a quicker method than conventional method to produce pure lines. However, its drawbacks of genotype-dependency and only a single chance of recombination limited its wider application. A recently developed fast generation cycling system (FGCS) achieved similar times to those of DH for the production of selfed pure lines but is more versatile as it is much less genotype-dependent than DH technology and does not restrict recombination to a single event. The advantages and disadvantages of the technologies and their produced pure line populations for different purposes of biological research and breeding are discussed. The development of a concept of complete in vitro meiosis and mitosis system is also proposed. This could integrate with the recently developed technologies of single cell genomic sequencing and genome wide selection, leading to a complete laboratory based pre-breeding scheme.

show abstract

Section: Rilsmentioning

confidence: 99%

Accelerated Generation of Selfed Pure Line Plants for Gene Identification and Crop Breeding

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Marker-assisted backcrossing for quality traits such as low cadmium content in durum wheat (Penner et al 1995;Knox et al 2009) and high grain protein content (Khan et al 2000) was also possible. More recently, the fine mapping of genes controlling quantitative traits (Cuthbert et al 2006) and the application of high throughput marker technologies (Somers & Humphreys 2009) have greatly expanded the applicability of MAB in plant breeding. A key to the successful integration of MAB has been development of markers tightly linked to the gene, "perfect" markers, and marker validation .…”

Section: Marker Assisted Breedingmentioning

confidence: 99%

“…Doubled haploid technology has been used to develop populations to study inheritance and develop molecular markers linked to traits (see reviews by Jauhar et al 2009;Somers & Humphreys 2009). DH technology achieves the production of true breeding lines in one generation.…”

Section: Doubled Haploid Technologymentioning

confidence: 99%

New breeding tools impact Canadian commercial farmer fields

DePauw¹,

Knox²,

Humphreys³

et al. 2011

CZECH J GENET PLANT

View full text Add to dashboard Cite

Abstract:The high cost of cultivar development encourages efficiencies to reduce time and costs to develop cultivars. Doubled haploid (DH) technology and marker assisted breeding (MAB) are two such tools that improve efficiencies. Since 1997, twenty five wheat cultivars in seven market classes, developed using DH methods, have been registered by the Canadian Food Inspection Agency. These DH cultivars accounted for more than one third of the Canadian wheat acreage in 2009. The DH cultivar Lillian, eligible for grades of Canada Western Red Spring class and currently the most widely grown wheat cultivar in Canada, was developed using MAB to improve grain protein content with the Gpc-B1/Yr36 on chromosome 6BS introgressed from Triticum turgidum L. (Zhuk.) dicoccoides (Körn. Ex Asch. & Graebn). AC Andrew, a Canada Western Soft White spring DH cultivar, was the most widely grown cultivar in its class for the last two years. The new market class, Canada Western Hard White Spring wheat, is based entirely on DH cultivars. Goodeve, one of the first Canada Western Red Spring cultivars released with the gene Sm1 on chromosome 2BS for resistance to the orange wheat blossom midge (Sitodiplosis mosellana (Géhin)) was selected by application of the DNA marker WM1. Glencross, the first cultivar in the Canada Western Extra Strong wheat class with Sm1, was selected using the WM1 marker on haploid plants prior to doubling. Development of the durum wheat cultivars CDC Verona and Brigade involved the use of a marker for Cdu1, a major gene on chromosome 5B that regulates grain cadmium concentration. Marker technology permits a more strategic and integrated approach to breeding by quantifying the introgression of various key genes into advanced breeding material, identifying targeted loci in parents and following up with MAB in the progeny.

show abstract

Doubled Haploid Breeding in Cereals

Humphreys

Knox

2015

Advances in Plant Breeding Strategies: Breeding, Biotechnology and Molecular Tools

View full text Add to dashboard Cite

State of QTL Detection and Marker‐Assisted Selection in Wheat Improvement

Cited by 3 publications

References 86 publications

Accelerated Generation of Selfed Pure Line Plants for Gene Identification and Crop Breeding

Accelerated Generation of Selfed Pure Line Plants for Gene Identification and Crop Breeding

New breeding tools impact Canadian commercial farmer fields

Doubled Haploid Breeding in Cereals

Contact Info

Product

Resources

About