Doubled Haploid Breeding in Cereals

Humphreys, D. G.; Knox, R. E.

doi:10.1007/978-3-319-22521-0_9

Cited by 32 publications

(27 citation statements)

References 101 publications

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“…A durum wheat population of 162 doubled haploid (DH) lines developed with the maize pollen method (Humphreys and Knox, 2015) and derived from Pelissier × Strongfield segregating for gluten strength was used in this study. Strongfield, selected from the cross AC Avonlea/DT665, is a registered Canada Western Amber Durum variety with strong gluten and low cadmium, developed at the Agriculture and Agri-Food Canada-Swift Current Research and Development Centre, Swift Current, SK (Clarke et al, 2005).…”

Section: Population and Field Trialsmentioning

confidence: 99%

High Density Mapping of Quantitative Trait Loci Conferring Gluten Strength in Canadian Durum Wheat

Ruan

Knox

et al. 2020

Front. Plant Sci.

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Gluten strength is one of the factors that determine the end-use quality of durum wheat and is an important breeding target for this crop. To characterize the quantitative trait loci (QTL) controlling gluten strength in Canadian durum wheat cultivars, a population of 162 doubled haploid (DH) lines segregating for gluten strength and derived from cv. Pelissier × cv. Strongfield was used in this study. The DH lines, parents, and controls were grown in 3 years and two seeding dates in each year and gluten strength of grain samples was measured by sodium dodecyl sulfate (SDS)-sedimentation volume (SV). With a genetic map created by genotyping the DH lines using the Illumina Infinium iSelect Wheat 90K SNP (single nucleotide polymorphism) chip, QTL contributing to gluten strength were detected on chromosome 1A, 1B, 2B, and 3A. Two major and stable QTL detected on chromosome 1A (QGlu.spa-1A) and 1B (QGlu.spa-1B.1) explaining 13.7-18.7% and 25.4-40.1% of the gluten strength variability respectively were consistently detected over 3 years, with the trait increasing alleles derived from Strongfield. Putative candidate genes underlying the major QTL were identified. Two novel minor QTL (QGlu.spa-3A.1 and QGlu.spa-3A.2) with the trait increasing allele derived from Pelissier were mapped on chromosome 3A explaining up to 8.9% of the phenotypic variance; another three minor QTL (QGlu.spa-2B.1, QGlu.spa-2B.2, and QGlu.spa-2B.3) located on chromosome 2B explained up to 8.7% of the phenotypic variance with the trait increasing allele derived from Pelissier. QGlu.spa-2B.1 is a new QTL and has not been reported in the literature. Multi-environment analysis revealed genetic (QTL) × environment interaction due to the difference of effect in magnitude rather than the direction of the QTL. Eleven pairs of digenic epistatic QTL were identified, with an epistatic effect between the two major QTL of QGlu.spa-1A and QGlu.spa-1B.1 detected in four out of six environments. The peak SNPs and SNPs flanking the QTL interval of QGlu.spa-1A and QGlu.spa-1B.1 were converted to Kompetitive Allele Specific PCR (KASP) markers, which can be deployed in marker-assisted breeding to increase the efficiency and accuracy of phenotypic selection for gluten strength in durum wheat. The QTL that were expressed consistently across

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Section: Population and Field Trialsmentioning

confidence: 99%

High Density Mapping of Quantitative Trait Loci Conferring Gluten Strength in Canadian Durum Wheat

Ruan

Knox

et al. 2020

Front. Plant Sci.

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“…Doubled haploids have been applied to rapidly achieve homozygosity in >250 plant species (Forster et al, 2015; Humphreys and Knox, 2015). By definition, DHs contain only additive genetic variation, including that arising from additive × additive epistasis, but none due to overdominant genetic variation (Snape, 1989).…”

Section: Hybrid‐enabled Line Profiling (Help)mentioning

confidence: 99%

“…The other two methods deliver near-homozygous lines, which for cultivar purposes are usually acceptable, but provide additional benefits. These methods are regularly reviewed, and new modifications are often published (Choo et al, 1985;Snape, 1989;Maluszynski et al, 2003;Forster et al, 2007;Touraev et al, 2009;Tadesse et al, 2012;Dwivedi et al, 2015;Humphreys and Knox, 2015).…”

Section: Move Quickly From the "Best" Hybrids To Derive Superior Homomentioning

confidence: 99%

Cross the Best with the Best, and Select the Best: HELP in Breeding Selfing Crops

Ginkel¹,

Ortíz

2018

Crop Science

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P lant breeding, certainly in the era before genomics and marker-assisted selection, was often referred to as a "numbers game." The more crosses, the more likely it is to find improved genetic combinations. We propose a newly integrated breeding strategy for self-fertilizing crops that dramatically reduces the number of crosses being promoted while increasing the likelihood of obtaining superior new cultivars. This strategy emerges from experiments in the Bread Wheat Program of the International Center for the Improvement of Maize and Wheat (CIMMYT).In the late 1990s we noticed that ~8% of the elite new lines in the International Bread Wheat Screening Nursery (IBWSN) for international distribution were derived from <20 crosses. The remaining 92% of elite lines came from 5000 to 10,000 crosses. These few original crosses, and the lines derived from them, represented a strategy that we now describe as hybrid-enabled line profiling (HELP). This strategy offers breeding programs for selfing crops enormous benefits through improved performance. It Cross the Best with the Best, and Select the Best: HELP in Breeding Selfing CropsMaarten van Ginkel* and Rodomiro Ortiz ABSTRACT Hybrid-enabled line profiling (HELP) is a new integrated breeding strategy for self-fertilizing crops that combines existing and recently identified elements, resulting in a strategy that synergistically exceeds existing breeding concepts. Heterosis in selfing crops is often driven by additive and additive ´ additive gene action, the molecular basis of which is increasingly being revealed. Unlike nonadditive heterosis, additive forms can be relatively easily fixed in homozygous lines, meaning that their seed can simply be resown to express the same "heterosis." Crossing diverse, complementary "selfing" parents to create the desired trait or allele line profile requires strict male sterility of the female; this can now be achieved relatively easily through present and emerging chemical, environmental, or genetic techniques. Fairly small amounts of hybrid seed are needed, with no need to scale up seed production, as it is not the hybrid that will be commercialized. After multilocation testing, homozygous lines from only the most superior hybrids, driven mainly by additive effects and additive ´ additive gene action, are rapidly derived using techniques such as doubled haploids. Multilocation testing and molecular confirmation of target line profiles then identify superior lines for release to farmers. The HELP strategy integrates modern high-throughput versions of existing and new concepts and methodologies into a breeding system strategy that focuses on the most superior crosses, <10% of all crosses. This focus results in significant increases in efficiency and can reverse the edible yield plateauing seen or feared in some of our major selfing food crops.M. van Ginkel,

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“…As the anthers from the F1 hybrid are generated after the meiosis, they represent the segregating haploid gametes. Therefore, the haploid plants regenerated from anther culture can be doubled by Colchicine treatment to generate homozygous lines (Jones et al 1997;Ortiz et al 2007;Humphreys and Knox 2016). On the other hand, F1…”

Section: Mapping Populationsmentioning

confidence: 99%

“…As part of the approach to improve the wheat breeding programs, initiatives such as shuttle breeding, double haploid technology, speed breeding, marker assisted breeding and transgenic technologies enables the rapid development of elite wheat lines (Ortiz et al 2007;Collard and Mackill 2008;Richardson et al 2014;Ishida et al 2015;Humphreys and Knox 2016;Ghosh et al 2018;Luo et al 2018;Watson et al 2018; Wulff and Dhugga 2018). However, unlocking traits in the complex wheat genome is essential to effectively use these breeding strategies.…”

Section: Key Advances In the Wheat Breeding For Trait Improvementmentioning

confidence: 99%

Molecular genetic characterisation of triple rust resistance in Aegilops tauschii

Athiyannan¹

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The major outcomes of this study include:  Understanding the genetic architecture of triple rust resistance in accession CPI110672  Cloning and validation of Sr46b and Yr672 rust resistance candidate genes  Fine mapping a leaf rust resistance (Lr39) gene  Development of breeder-friendly molecular markers for Sr46, Yr672 and Lr39  Marker assisted introgression of Sr46b and Yr672 into a commercial cultivar  Marker assisted pyramiding of Sr33/Sr45/Sr46b resistance genes using speed breeding technology v Declaration by author This thesis is composed of my original work, and contains no material previously published or written by another person except where due reference has been made in the text. I have clearly stated the contribution by others to jointly-authored works that I have included in my thesis. I have clearly stated the contribution of others to my thesis as a whole, including statistical assistance, survey design, data analysis, significant technical procedures, professional editorial advice, financial support and any other original research work used or reported in my thesis. The content of my thesis is the result of work I have carried out since the commencement of my higher degree by research candidature and does not include a substantial part of work that has been submitted to qualify for the award of any other degree or diploma in any university or other tertiary institution. I have clearly stated which parts of my thesis, if any, have been submitted to qualify for another award. I acknowledge that an electronic copy of my thesis must be lodged with the University Library and, subject to the policy and procedures of The University of Queensland, the thesis be made available for research and study in accordance with the Copyright Act 1968 unless a period of embargo has been approved by the Dean of the Graduate School.

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Doubled Haploid Breeding in Cereals

Cited by 32 publications

References 101 publications

High Density Mapping of Quantitative Trait Loci Conferring Gluten Strength in Canadian Durum Wheat

High Density Mapping of Quantitative Trait Loci Conferring Gluten Strength in Canadian Durum Wheat

Cross the Best with the Best, and Select the Best: HELP in Breeding Selfing Crops

Molecular genetic characterisation of triple rust resistance in Aegilops tauschii

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