STATc is a key regulator of the transcriptional response to hyperosmotic shock

Na, Jianbo; Tunggal, Budi; Eichinger, Ludwig M.

doi:10.1186/1471-2164-8-123

Cited by 28 publications

(37 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The microarray experiments were performed and data were analyzed essentially as described previously [18], [20]. For each set of experiments, we used three independent RNA preparations, hybridized six microarrays, and swapped the dyes in the labeling reaction to exclude dye-specific errors.…”

Section: Methodsmentioning

confidence: 99%

The Carboxy-Terminal Domain of Dictyostelium C-Module-Binding Factor Is an Independent Gene Regulatory Entity

Lucas¹,

Bilzer²,

Moll

et al. 2009

PLoS ONE

Self Cite

View full text Add to dashboard Cite

The C-module-binding factor (CbfA) is a multidomain protein that belongs to the family of jumonji-type (JmjC) transcription regulators. In the social amoeba Dictyostelium discoideum, CbfA regulates gene expression during the unicellular growth phase and multicellular development. CbfA and a related D. discoideum CbfA-like protein, CbfB, share a paralogous domain arrangement that includes the JmjC domain, presumably a chromatin-remodeling activity, and two zinc finger-like (ZF) motifs. On the other hand, the CbfA and CbfB proteins have completely different carboxy-terminal domains, suggesting that the plasticity of such domains may have contributed to the adaptation of the CbfA-like transcription factors to the rapid genome evolution in the dictyostelid clade. To support this hypothesis we performed DNA microarray and real-time RT-PCR measurements and found that CbfA regulates at least 160 genes during the vegetative growth of D. discoideum cells. Functional annotation of these genes revealed that CbfA predominantly controls the expression of gene products involved in housekeeping functions, such as carbohydrate, purine nucleoside/nucleotide, and amino acid metabolism. The CbfA protein displays two different mechanisms of gene regulation. The expression of one set of CbfA-dependent genes requires at least the JmjC/ZF domain of the CbfA protein and thus may depend on chromatin modulation. Regulation of the larger group of genes, however, does not depend on the entire CbfA protein and requires only the carboxy-terminal domain of CbfA (CbfA-CTD). An AT-hook motif located in CbfA-CTD, which is known to mediate DNA binding to A+T-rich sequences in vitro, contributed to CbfA-CTD-dependent gene regulatory functions in vivo.

show abstract

Section: Methodsmentioning

confidence: 99%

The Carboxy-Terminal Domain of Dictyostelium C-Module-Binding Factor Is an Independent Gene Regulatory Entity

Lucas¹,

Bilzer²,

Moll

et al. 2009

PLoS ONE

Self Cite

View full text Add to dashboard Cite

show abstract

“…Na, Tunggal, & Eichinger () examined the transcriptional changes during hypertonic stress in D. discoideum and identified 809 genes that were differentially expressed. Utilizing the Gene Ontology (GO) database, we identified an enrichment (binomial test) of genes encoding CaMBPs (GO: 0005516): rgaA , mhcA , myoA , cdk5 , thyB , canA , and uncharacterized gene DDB_G0269864 (Fig.…”

Section: Osmoregulation and Vacuole Functionmentioning

confidence: 99%

Calmodulin‐mediated events during the life cycle of the amoebozoan Dictyostelium discoideum

et al. 2019

View full text Add to dashboard Cite

This review focusses on the functions of intracellular and extracellular calmodulin, its target proteins and their binding proteins during the asexual life cycle of Dictyostelium discoideum. Calmodulin is a primary regulatory protein of calcium signal transduction that functions throughout all stages. During growth, it mediates autophagy, the cell cycle, folic acid chemotaxis, phagocytosis, and other functions. During mitosis, specific calmodulin‐binding proteins translocate to alternative locations. Translocation of at least one cell adhesion protein is calmodulin dependent. When starved, cells undergo calmodulin‐dependent chemotaxis to cyclic AMP generating a multicellular pseudoplasmodium. Calmodulin‐dependent signalling within the slug sets up a defined pattern and polarity that sets the stage for the final events of morphogenesis and cell differentiation. Transected slugs undergo calmodulin‐dependent transdifferentiation to re‐establish the disrupted pattern and polarity. Calmodulin function is critical for stalk cell differentiation but also functions in spore formation, events that begin in the pseudoplasmodium. The asexual life cycle restarts with the calmodulin‐dependent germination of spores. Specific calmodulin‐binding proteins as well as some of their binding partners have been linked to each of these events. The functions of extracellular calmodulin during growth and development are also discussed. This overview brings to the forefront the central role of calmodulin, working through its numerous binding proteins, as a primary downstream regulator of the critical calcium signalling pathways that have been well established in this model eukaryote. This is the first time the function of calmodulin and its target proteins have been documented through the complete life cycle of any eukaryote.

show abstract

“…6B). In contrast, stress-induced STATc activation (Araki et al, 2003; Na et al, 2007), was not affected in lkb1 knockdown cells (Fig. 6B).…”

Section: Resultsmentioning

confidence: 86%

LKB1 regulates development and the stress response in Dictyostelium

Veeranki

Hwang

Sun

et al. 2011

Developmental Biology

View full text Add to dashboard Cite

The serine/threonine kinase LKB1 is a master kinase that regulates a number of critical events such as cell transformation, polarization, development, stress response, and energy metabolism in metazoa. After multiple unsuccessful attempts of generating Dictyostelium lkb1-null cells, an RNAi-based knockdown approach proved effective. Depletion of lkb1 with a knockdown construct displayed severe reduction in prespore cell differentiation and precocious induction of prestalk cells, which were reminiscent of cells lacking GSK3. Similarly to gsk3− cells, lkb1 depleted cells displayed lower GSK3 activity than wild type cells during development and compromised cAMP-mediated inhibition of the DIF-1 mediated ecmB induction. In response to stress insult, the kinase activity of LKB1, but not that of GSK3, increased. Therefore, LKB1 positively functions at the upstream of GSK3 during development and responds to stress insults independently from GSK3.

show abstract

STATc is a key regulator of the transcriptional response to hyperosmotic shock

Cited by 28 publications

References 61 publications

The Carboxy-Terminal Domain of Dictyostelium C-Module-Binding Factor Is an Independent Gene Regulatory Entity

The Carboxy-Terminal Domain of Dictyostelium C-Module-Binding Factor Is an Independent Gene Regulatory Entity

Calmodulin‐mediated events during the life cycle of the amoebozoan Dictyostelium discoideum

LKB1 regulates development and the stress response in Dictyostelium

Contact Info

Product

Resources

About