STAT1 dissociates adipose tissue inflammation from insulin sensitivity in obesity

Cox, Aaron R.; Chernis, Natasha; Bader, David A.; Saha, Pradip Kumar; Masschelin, Peter M.; Felix, Jessica B.; Lian, Zeqin; Putluri, Vasanta; Rajapakshe, Kimal I.; Kim, Kang Ho; Villareal, Dennis T.; Armamento-Villareal, Reina; Wu, Huaizhu; Coarfa, Cristian; Putluri, Nagireddy; Hartig, Sean M.

doi:10.1101/2020.04.10.036053

Cited by 1 publication

(1 citation statement)

References 56 publications

(65 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Obesity often causes chronic inflammation, and related studies have revealed that obesity, low-intensity chronic inflammation, and insulin resistance are closely associated with each other [29,30]. Adipose tissues release various adipokines involved in energy homeostasis that interact with macrophages and play a crucial role in the inflammatory response in adipose tissues.…”

Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory and Cytotoxicity Effects of Cudrania tricuspidata Fruits Vinegar in a Co-Culture System with RAW264.7 Macrophages and 3T3-L1 Adipocytes

Choi

Park

Yeo

et al. 2020

Foods

View full text Add to dashboard Cite

Vinegar has been found to have in vitro improvement effect on inflammatory biomarkers, and clinically used to improve inflammation and obesity-related diseases. This study was designed to analyze in vitro anti-inflammatory effects of Cudrania tricuspidata fruits vinegar (CTFV) in a co-culture system with macrophages and adipocytes. We analyzed the physicochemical properties and polyphenolic ingredients of CTFV, and investigated in vitro anti-inflammatory effects of CTFV in a co-culture system with macrophages and adipocytes. The cells were cultured in the presence of CTFV for 24 h in contact with each other, then, harvested. The levels of monocyte chemoattractant protein (MCP)-1, tumour necrosis factor (TNF)-α, inducible nitric oxide synthase (iNOS), nitric oxide (NO), and interleukin (IL)-6 were evaluated by using the Griess reagent, western blot, or enzyme-linked immunosorbent assay assays. We found that increasing levels for NO, iNOS, TNF-α, IL-6 and MCP-1 were caused by LPS treatment and co-culture using the contact method, whereas CTFV efficaciously attenuated inflammatory response by improving inflammatory parameters including NO, iNOS, TNF-α, IL-6 and MCP-1. The present study indicates that CTFV might provide a nutraceutical product or functional food resource for improving inflammation processed via the interaction of adipocytes and macrophages.

show abstract

Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory and Cytotoxicity Effects of Cudrania tricuspidata Fruits Vinegar in a Co-Culture System with RAW264.7 Macrophages and 3T3-L1 Adipocytes

Choi

Park

Yeo

et al. 2020

Foods

View full text Add to dashboard Cite

show abstract

STAT1 dissociates adipose tissue inflammation from insulin sensitivity in obesity

Abstract: 25Obesity fosters low-grade inflammation in white adipose tissue (WAT) that may contribute 26 to the insulin resistance that characterizes type 2 diabetes mellitus (T2DM). However, the 27 Research). cDNA was synthesized using SuperScript VILO Master Mix (Thermo Fisher). 132

Cited by 1 publication

References 56 publications

Anti-Inflammatory and Cytotoxicity Effects of Cudrania tricuspidata Fruits Vinegar in a Co-Culture System with RAW264.7 Macrophages and 3T3-L1 Adipocytes

Anti-Inflammatory and Cytotoxicity Effects of Cudrania tricuspidata Fruits Vinegar in a Co-Culture System with RAW264.7 Macrophages and 3T3-L1 Adipocytes

Contact Info

Product

Resources

About