Starvation survival of the fish pathogenic bacteria Vibrio anguillarum and Vibrio salmonicida in marine environments

Enger, O

doi:10.1016/0378-1097(90)90555-5

Cited by 2 publications

(2 citation statements)

References 4 publications

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“…After the initial hundredfold increases in plate counts in all media, plate counts decreased markedly in COMB-salt solution with glucose but remained relatively constant in the other media. There was a Ͼ100-fold difference in plate counts of samples from COMB-salt solution with glucose and the other media after incubation for 113 h. An initial increase in plate counts followed by maintenance at the increased level has been previously reported to occur in starvation media (1,5,13), but a decrease in plate counts in the presence of glucose is a novel observation. Glutamate and phosphate were effective in preventing the inhibitory effect of glucose (see Tables 5 and 7).…”

supporting

confidence: 49%

Decrease in culturability of Vibrio cholerae caused by glucose

Shiba¹,

Hill²,

Straube³

et al. 1995

Appl Environ Microbiol

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The culturability of Vibrio cholerae O1 serotype Inaba strain 569B was decreased by the addition of glucose to cell suspensions in starvation media. A similar effect was observed with sucrose, maltose, and fructose. We term this inhibitory effect glucose shock. It was not observed with arabinose or xylose or with carboxylates, such as acetate and pyruvate. No acidification of the medium occurred in the presence of these carbohydrates. Glucose shock was prevented by the addition of nitrogen or phosphorus sources. In the presence of phosphate, the bacterium produced formic acid from glucose. The phenomenon of glucose shock was also observed in V. cholerae O1 serotype Inaba strain RIMD 2203082 but not in strain RIMD 2203088 (O1 Inaba), IID 936 (O1 Ogawa), or RIMD 2214034 (non-O1). The culturability of Escherichia coli, Enterobacter aerogenes, and Listonella anguillarum did not decrease in starvation media with added glucose. Hence, the phenomenon should have ecological significance in determining the distribution of bacteria in marine ecosystems in situations where carbohydrates are abundant, but nitrogen and phosphorus are limiting. MATERIALS AND METHODS Bacterial strains and culture conditions. V. cholerae Inaba strains 569B, RIMD 2203082 and RIMD 2203088, Ogawa strain IID 936, and non-O1 strain RIMD 2214034 were grown aerobically at 30ЊC in COMB medium, which contained the following (per liter of deionized water): NaCl, 20.0 g; MgSO 4 ⅐ 7H 2 O, 0.5 g; KCl, 0.1 g; CaCl 2 , 0.1 g; D-glucose, 2.0 g; L-aspartic acid, 1.0 g; L-glutamic acid, 1.0 g; ferric citrate, 1.0 mg; a vitamin mixture, 1.0 ml; and a trace element solution, 0.1 ml. The vitamin mixture contained the following (per milliliter of deionized water): biotin, 0.1 mg; thiamine HCl, 10.0 mg; nicotinic acid, 10.0 mg; Na-pantothenate, 10.0 mg; and vitamin B 12 , 0.1 mg. The trace element solution employed in the study contained the following (per milliliter of deionized water): ZnSO 4 , 0.7 mg; MnCl 2 , 1.0 mg; H 3 BO 3 , 0.6 mg; CoCl 2 , 2.0 mg; CuSO 4 , 0.2 mg; NiCl 2 ⅐ 6H 2 O, 0.2 mg; Na 2 MoO 4 ⅐ 2H 2 O, 0.4 mg; and 25% HCl, 10.0 l. The pH was adjusted to 7.5 with 20 mM Tris-HCl. This solution is a slight modification of SL7 (7). An equimolar mixture of NaH 2 PO 4 and Na 2 HPO 4 was used as a phosphorus source at a final concentration of 0.2 mM. D-Glucose, phosphate, and vitamin solutions were filter sterilized and added separately to prepared media. V. cholerae showed a constant growth yield during serial culture on this medium. Listonella anguillarum (IFO12710), also included in this study, was grown on PY medium, which is a modified COMB medium containing 1 g of Polypeptone (Difco) and 0.2 g of yeast extract (Difco) in place of glucose, amino acids, and the vitamin mixture. Other strains included for purposes of comparison were Enterobacter aerogenes IFO13534 and Escherichia coli IFO3301, grown on modified PY medium in which NaCl was reduced to 0.5 g/liter. Growth with different nitrogen sources. Assimilation of different nitrogen sources was examined...

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supporting

confidence: 49%

Decrease in culturability of Vibrio cholerae caused by glucose

Shiba¹,

Hill²,

Straube³

et al. 1995

Appl Environ Microbiol

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“…However, when released into a freshwater environment, V. anguillarum will experience both low osmotic stress and nutrient starvation. Although V. anguillarum can maintain its colony forming ability for more than several months in brackish and marine environments, it rapidly loses the ability to form colonies on plates in freshwater environment [3, 6–8].…”

Section: Introductionmentioning

confidence: 99%

Response to low osmotic stress in a fish pathogen, Vibrio anguillarum

Miyamoto

Eguchi

2006

FEMS Microbiology Ecology

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Low osmotic stress responses of the fish pathogen, Vibrio anguillarum, were investigated and characterized with regard to changes in cell density and cation type in natural lake water (aged lake water, ALW) and MilliQ water (MQW). This pathogen showed higher survival (% CFU) in ALW than in MQW at all the examined cell densities (106–109 CFU ml−1). Higher resistance in ALW was partially due to divalent cations such as Ca2+ and Mg2+ present in ALW. It was also observed that addition of the supernatant, which was obtained from the pre‐suspension of V. anguillarum cells in ALW, significantly increased the survival of the pathogen in ALW. This indicated that the extracellular substance(s) released from the pathogen also played an effective role(s) for survival in ALW. Thus, divalent cations and cell density assist the pathogen, V. anguillarum, to overcome the low osmotic stress in natural freshwater environments.

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Starvation survival of the fish pathogenic bacteria Vibrio anguillarum and Vibrio salmonicida in marine environments

Cited by 2 publications

References 4 publications

Decrease in culturability of Vibrio cholerae caused by glucose

Decrease in culturability of Vibrio cholerae caused by glucose

Response to low osmotic stress in a fish pathogen, Vibrio anguillarum

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