Startup and oxygen concentration effects in a continuous granular mixed flow autotrophic nitrogen removal reactor

Varas, Rodrigo; Guzmán‐Fierro, Víctor; Giustinianovich, Elisa; Behar, Jack; Fernández, Katherina; Roeckel, Marlene

doi:10.1016/j.biortech.2015.04.086

Cited by 59 publications

(36 citation statements)

References 30 publications

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“…The inoculum was preacclimated granular biomass from a PN‐A reactor with synthetic substrate. Details can be found in Varas et al . The initial biomass concentration was 3.02 g volatile suspended solids (VSS) L −1 .…”

Section: Methodsmentioning

confidence: 99%

“…The inlet and outlet streams were controlled with a two‐headed peristaltic pump. An oxygen control system was implemented according to the strategy proposed by Varas et al . The same reactor was used in the work presented by Arriagada et al …”

Section: Methodsmentioning

confidence: 99%

“…Samples of influent and effluent of the PN‐A reactor were collected during all stages of evaluation . Nitrogen compounds [nitrate, nitrite and total ammonium nitrogen (TAN‐N)], total suspended solids (TSS), VSS and the pH were analyzed according to the tests proposed by Arriagada et al …”

Section: Methodsmentioning

confidence: 99%

“…Respirometric batch experiments were performed to measure specific nitrification activity (SNA) according to the procedure described by Arriagada et al . Specific Anammox activity (SAA) was measured based on increased pressure using OxiTop® Control AN6 (WTW, Weilheim, Germany) according to Varas et al …”

Section: Methodsmentioning

confidence: 99%

“…The control of dissolved oxygen (DO) is of overriding importance to prevent the growth of nitrite oxidizing bacteria (NOB) and to avoid the inhibition of anAOB. NOB has lower affinity for oxygen than aerAOB and it competes for nitrite with anAOB . This factor led to the first start‐up strategies of the PN‐A process, which were focused on acclimation of the biomass to low oxygen concentrations.…”

Section: Introductionmentioning

confidence: 99%

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Low oxygen start‐up of partial nitrification‐anammox process: mechanical or gas agitation?

Jara‐Muñoz

Guzmán‐Fierro

Arriagada

et al. 2018

J of Chemical Tech & Biotech

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BACKGROUND Partial nitrification‐anammox (PN‐A) is a widely recognized technology to remove nitrogen from different types of wastewater. Low oxygen concentration is the most used strategy for PN‐A start‐up, but stability problems arise during the operation; thus, in the present study the effects of the type of agitation, oxygenation and shear stress on the sensitivity, energy consumption and performance were evaluated. Recognition of these parameters allows considered choice of the design of an industrial process for nitrogen abatement. RESULTS A mechanically agitated reactor (MAR) was compared to a stable, long‐term operation period bubble column reactor (BCR), both started under low dissolved oxygen concentration conditions. MAR microbial assays confirmed the destruction of the nitrifying layer and an imbalance of the entire process when the oxygen to nitrogen loading ratio (O2:N) decreased by 25%. The granule sedimentation rate and specific anammox activity were 17% and 87% higher (respectively) in BCR. Economic analysis determined that the cost of aeration for the MAR and for the BCR were 23.8% and 1% of the total PN‐A energy consumption, respectively. CONCLUSIONS The BCR showed better results than the MAR. This study highlights the importance of type of agitation, oxygenation and shear stress for industrial‐scale PN‐A designs. © 2018 Society of Chemical Industry

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Low oxygen start‐up of partial nitrification‐anammox process: mechanical or gas agitation?

Jara‐Muñoz

Guzmán‐Fierro

Arriagada

et al. 2018

J of Chemical Tech & Biotech

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Effect of dissolved oxygen on elemental sulfur generation in sulfide and nitrate removal process: characterization, pathway, and microbial community analysis

Wang

Zhang

2015

Appl Microbiol Biotechnol

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Microaerobic bioreactor treatment for enriched sulfide and nitrate has been demonstrated as an effective strategy to improve the efficiencies of elemental sulfur (S(0)) generation, sulfide oxidation, and nitrate reduction. However, there is little detailed information for the effect and mechanism of dissolved oxygen (DO) on the variations of microbial community in sulfur generation, sulfide oxidation, and nitrate reduction systems. Polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) was employed to evaluate the variations of microbial community structures in a sulfide oxidation and nitrate reduction reactor under different DO conditions (DO 0-0.7 mg · L(-1)). Experimental results revealed that the activity of sulfide-oxidizing bacteria (SOB) and nitrate-reducing bacteria (NRB) could be greatly stimulated in 0.1-0.3 mg-DO · L(-1). However, when the DO concentration was further elevated to more than 0.5 mg · L(-1), the abundance of NRB was markedly decreased, while the heterotrophic microorganisms, especially carbon degradation species, were enriched. The reaction pathways for sulfide and nitrate removal under microaerobic conditions were also deduced by combining batch experiments with functional species analysis. It was likely that the oxidation of sulfide to sulfur could be performed by both aerobic heterotrophic SOB and sulfur-based autotrophic denitrification bacteria with oxygen and nitrate as terminal electron acceptor, respectively. The nitrate could be reduced to nitrite by both autotrophic and heterotrophic denitrification, and then the generated nitrite could be completely converted to nitrogen gas via heterotrophic denitrification. This study provides new insights into the impacts of microaerobic conditions on the microbial community functional structures of sulfide-oxidizing, nitrate-reducing, and sulfur-producing bioreactors, which revealing the potential linkage between functional microbial communities and reactor performance.

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Single-stage autotrophic nitrogen removal process at high loading rate: granular reactor performance, kinetics, and microbial characterization

Qian

Gebreyesus

Wang

et al. 2018

Appl Microbiol Biotechnol

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For the possible highest performance of single-stage combined partial nitritation/anammox (PNA) process, a continuous complete-mix granular reactor was operated at progressively higher nitrogen loading rate. The variations in bacterial community structure of granules were also characterized using high-throughput pyrosequencing, to give a detail insight to the relationship between reactor performance and functional organism abundance within completely autotrophic nitrogen removal system. In 172 days of operation, a superior total nitrogen (TN) removal rate over 3.9 kg N/(m/day) was stable implemented at a fixed dissolved oxygen concentration of 1.9 mg/L, corresponding to the maximum specific substrate utilization rate of 0.36/day for TN based on the related kinetics modeling. Pyrosequencing results revealed that the genus Nitrosomonas responsible for aerobic ammonium oxidation was dominated on the granule surface, which was essential to offer the required niche for the selective enrichment of anammox bacteria (genus Candidatus Kuenenia) in the inner layer. And the present of various heterotrophic organisms with general functions, known as fermentation and denitrification, could not be overlooked. In addition, it was believed that an adequate excess of ammonium in the bulk liquid played a key role in maintaining process stability, by suppressing the growth of nitrite-oxidizing bacteria through dual-substrate competitions.

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Startup and oxygen concentration effects in a continuous granular mixed flow autotrophic nitrogen removal reactor

Cited by 59 publications

References 30 publications

Low oxygen start‐up of partial nitrification‐anammox process: mechanical or gas agitation?

Low oxygen start‐up of partial nitrification‐anammox process: mechanical or gas agitation?

Effect of dissolved oxygen on elemental sulfur generation in sulfide and nitrate removal process: characterization, pathway, and microbial community analysis

Single-stage autotrophic nitrogen removal process at high loading rate: granular reactor performance, kinetics, and microbial characterization

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