Effect of dissolved oxygen on elemental sulfur generation in sulfide and nitrate removal process: characterization, pathway, and microbial community analysis

Abstract

Microaerobic bioreactor treatment for enriched sulfide and nitrate has been demonstrated as an effective strategy to improve the efficiencies of elemental sulfur (S(0)) generation, sulfide oxidation, and nitrate reduction. However, there is little detailed information for the effect and mechanism of dissolved oxygen (DO) on the variations of microbial community in sulfur generation, sulfide oxidation, and nitrate reduction systems. Polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) was employed to evaluate the variations of microbial community structures in a sulfide oxidation and nitrate reduction reactor under different DO conditions (DO 0-0.7 mg · L(-1)). Experimental results revealed that the activity of sulfide-oxidizing bacteria (SOB) and nitrate-reducing bacteria (NRB) could be greatly stimulated in 0.1-0.3 mg-DO · L(-1). However, when the DO concentration was further elevated to more than 0.5 mg · L(-1), the abundance of NRB was markedly decreased, while the heterotrophic microorganisms, especially carbon degradation species, were enriched. The reaction pathways for sulfide and nitrate removal under microaerobic conditions were also deduced by combining batch experiments with functional species analysis. It was likely that the oxidation of sulfide to sulfur could be performed by both aerobic heterotrophic SOB and sulfur-based autotrophic denitrification bacteria with oxygen and nitrate as terminal electron acceptor, respectively. The nitrate could be reduced to nitrite by both autotrophic and heterotrophic denitrification, and then the generated nitrite could be completely converted to nitrogen gas via heterotrophic denitrification. This study provides new insights into the impacts of microaerobic conditions on the microbial community functional structures of sulfide-oxidizing, nitrate-reducing, and sulfur-producing bioreactors, which revealing the potential linkage between functional microbial communities and reactor performance.

Effects of organic carbon consumption on denitrifier community composition and diversity along dissolved oxygen vertical profiles in lake sediment surface

“…The change in DO concentration can alter the bacterial community structure. When the DO concentration is from 0-0.7 mg/L, then variations of microbial community structures can be realized in a sulfi de oxidation and nitrate reduction reactor (Wang et al, 2016). The 2.5 mg/L DO concentration also ensures the optimal bacterial community in a moving bed sequencing batch reactor (Cao et al, 2017).…”

H2S biotreatment with sulfide-oxidizing heterotrophic bacteria

“…and green sulfur bacterium (Pokorna and Zabranska 2015 ). A problem of using these sulfur-oxidizing bacteria is the production of sulfuric acid, leading to acidification of the liquid phase; however, the problem can be prevented if oxygen supply is restricted (Dolejs et al 2015 ; Janssen et al 1998 ; Sorokin et al 2008 ; Wang et al 2016 ). When acidification occurs, alkaline materials are added to neutralize the acidified liquid, which increases the cost (Gerrity et al 2016 ; Mora et al 2015 ; Pokorna and Zabranska 2015 ).…”

Eco-friendly Industrial wastewater treatment: Potential of mesophilic bacterium, Pseudomonas putida (ATCC 49128) for hydrogen sulfide oxidation

“…Analysis of the samples for the quantification of sulfide depletion was as described elsewhere [13]. The methyl blue method in Hach (2800DR) spectrophotometer was used to analyze sulfide [14].…”