H2S biotreatment with sulfide-oxidizing heterotrophic bacteria

Abstract

Many industrial activities produce H2S, which is toxic at high levels and odorous at even very low levels. Chemolithotrophic sulfur-oxidizing bacteria are often used in its remediation. Recently, we have reported that many heterotrophic bacteria can use sulfide:quinone oxidoreductase and persulfide dioxygenase to oxidize H2S to thiosulfate and sulfite. These bacteria may also potentially be used in H2S biotreatment. Here we report how various heterotrophic bacteria with these enzymes were cultured with organic compounds and the cells were able to rapidly oxidize H2S to zero-valence sulfur and thiosulfate, causing no apparent acidification. Some also converted the produced thiosulfate to tetrathionate. The rates of sulfide oxidation by some of the tested bacteria in suspension, ranging from 8 to 50 µmol min−1 g−1 of cell dry weight at pH 7.4, sufficient for H2S biotreatment. The immobilized bacteria removed H2S as efficiently as the bacteria in suspension, and the inclusion of Fe3O4 nanoparticles during immobilization resulted in increased efficiency for sulfide removal, in part due to chemical oxidation H2S by Fe3O4. Thus, heterotrophic bacteria may be used for H2S biotreatment under aerobic conditions.Electronic supplementary materialThe online version of this article (10.1007/s10532-018-9849-6) contains supplementary material, which is available to authorized users.

Aerobic Denitrification and Heterotrophic Sulfur Oxidation in the Genus Halomonas Revealed by Six Novel Species Characterizations and Genome-Based Analysis

“…Another important trait of all these isolates was their ability for heterotrophic sulfide oxidation. As shown in Figure 5A and Supplementary Table 4, strains MCCC 1A11059 T and MCCC 1A13718 T exhibited the strongest sulfide oxidation ability under aerobic conditions; they could completely oxidize 1 mM of Na 2 S within 1 h, with sulfide oxidation rates reaching 50 and 54 µmol min −1 g −1 of cell dry weight, respectively, which were comparable with those of other reported heterotrophic sulfide oxidizers (Hou et al, 2018). Thiosulfate was identified as one of the main products; however, no sulfite and sulfate could be detected ( Figure 5B).…”

“…Sulfide and thiosulfate oxidation tests were carried out according to previously described methods ( Hou et al, 2018 ). Bacteria were cultivated to stationary phase, harvested by centrifugation (6,000 g, 5 min), and resuspended in 50 mM of HEPES buffer (pH 7.5) to a turbidity of 2 at 600 nm.…”

Sulfide removal using immobilized living cell in alginate matrices in anaerobic condition

“…Based on data, it was obtained that AL matrix could oxidize 0.2 mM sulfide with the removal rate of 0.103 mM/day and produced sulfate with 0.014 mM/day. The trend of the stable pH and low sulfate production explained that the product of sulfide oxidation in the experiment mainly was sulfur or another sulfide oxidized form [9].…”

Effective Sulfur Oxidizing Bacterial Isolation From Process Waste Water of Food Industry

“…The biogas product typical present of CH 4 (53-70 %), CO 2 (30-47 %), N 2 (0-3 %), H 2 O (5-10 %), O 2 (0-1 %), H 2 S (0-10,000 ppmv), NH 3 (0-100 ppmv), hydrocarbons (0-200 mg m -3 ) and siloxanes (0-41 mg m -3 ) constituents (Promuan and Thong, 2017). The amount of H 2 S varies according to composition of substrate used for biogas production (Hou et al, 2018). Hydrogen sulphide from biogas should be removed by pre-treatment to clean biogas and prevent corrosion of gas engine.…”

“…The one group of Archaea has aerobic sulfur oxidizers that are members of Sulfolobales, while in the other group of Bacteria include sulfur oxidizers aerobic lithotrophs or anaerobic phototrophs. For example members of the genus Bacillus, Beggiatoa, Thiothrix, Thermothix, Thiovolum, Acidianus, Sulfolobus, Thioalcalimicrobium, Thioalkallividrio, and Thiobacillus can be classified as Acidithiobacillus, Thermithiobacillus, and Halothiobacillus (Ravichandra et al, 2007;Rojas-Avelizapa et al, 2013;Hou et al, 2018;Behera et al, 2014).…”