Abstract-Monosodium glutamate (MSG) wastewater is a very refractory case of high strength organic wastewater. To investigate the effect of operating variables on treatment of MSG wastewater, a laboratory scale study was conducted. The treatment of MSG wastewater was studied by UASB-A/OMBR process. The effect of operating variables such as organic volumetric load rate (OLR), pH, volatile fatty acids (VFA) , ammonia (NH 3 -N) and alkalinity on efficiency of treatment and recovery of biogas was investigated. The results showed that, in the UASB stage, the gas production rate was 3.02 -8.83 kg COD / m 3 •d volume load, the gas production rate and COD load volume was positively related; as influent COD in range of 11878 -16922 mg•l -1 , COD removal rate of effluent was in range of 57.00 -79.63 %. The removal of COD by MBR can reach 90%, even to 96%, and the removal of ammonia is 95% -100% . UASB--A/O MBR has a good ability of preventing fluctuation of load and can remove COD and ammonia effectively.