Starch Measurement in Plant Tissue Using Enzymatic Hydrolysis

Haissig, Bruce E.; Dickson, Richard E.

doi:10.1111/j.1399-3054.1979.tb03207.x

Cited by 220 publications

(122 citation statements)

References 14 publications

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“…The concentration of total non-structural carbohydrates (TNC -soluble carbohydrates and starch) was determined according to the method as described by Haissig and Dickson (1979) and Hansen and Møller (1975) with some modifications. The extraction of soluble carbohydrates was performed 3 times in a mixture of methanol:chloroform:water (12:5:3 by volume) (Avantor Performance Materials, Poland) and starch was determined in the precipitate remaining after extraction.…”

Section: Carbohydrate Analysismentioning

confidence: 99%

Effect of seed storage temperature on fine root development and mycorrhizal colonization of young Populus nigra seedlings

Mucha

Szymańska

Zadworny

et al. 2015

Annals of Forest Science

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Key message Seed storage temperature influences root anatomy of the endangered Populus nigra, and consequently may alter nutrient absorption. A lower temperature during seed storage (−20 and −196 °C) may preserve the potential for a suitable root system development after germination. Context Seed storage conditions can be an important determinant of later seedling growth of Populus nigra L., an endangered tree species. Aims We tested whether long-term seed storage temperature, −10, −20 or −196 °C, affects the pattern of seedling root traits responsible for resource acquisition as compared to seedlings of fresh seeds. Methods We analysed the morphology, anatomy, degree of mycorrhizal colonization, and biochemical composition of roots developed from seed stored for 24 months at five different temperatures (from 3 to −196 °C) commonly used to preserve genetic resources. Results Except for root anatomy, we found no relationship between seed storage temperature and the root traits of seedlings. Among the various storage conditions, the proportion of roots with primary development in the first four orders was similar in seedlings developed from fresh seeds of from seeds stored at −196 or −20 °C. Nitrogen content in the roots was positively correlated with the proportion of (i) roots with primary development and (ii) the cortex width in the root diameter. Conclusions Higher temperatures during seed storage reduced the proportion of roots with absorptive function (with primary development). Therefore, for preservation of P. nigra seeds we recommend lower temperatures such as −20 and −196 °C.

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Section: Carbohydrate Analysismentioning

confidence: 99%

Effect of seed storage temperature on fine root development and mycorrhizal colonization of young Populus nigra seedlings

Mucha

Szymańska

Zadworny

et al. 2015

Annals of Forest Science

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“…Following extraction, soluble sugars were determined colorimetrically following digestion with sulfuric acid (Hanson & Mo$ ller, 1975). Starch content was determined colorimetrically following hot-water extraction of the residual sample tissue and enzymatic digestion of the extract (Haissig & Dickson, 1979). All data for this experiment were analysed using ANOVA and regression procedures of the JMP statistical analysis software (version 3.1, SAS Institute, Cary, NC, USA).…”

Section: Treatments and Samplingmentioning

confidence: 99%

The effect of defoliation intensity and history on photosynthesis, growth and carbon reserves of two conifers with contrasting leaf lifespans and growth habits

Vanderklein

1999

New Phytologist

104

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The effects of partial defoliation on photosynthesis, whole-seedling carbon allocation, partitioning and growth were studied for two species with contrasting foliar traits. Field-grown seedlings of deciduous Japanese larch (Larix leptolepis) and evergreen red pine (Pinus resinosa) were defoliated by hand in early summer for 2 consecutive years. In the first year (1990), seedlings were defoliated by removing the distal 0, 25, 50 or 75% of each needle. In the second year (1991), seedlings were defoliated either 0 or 50%, regardless of previous defoliation treatments. Defoliation had little effect on photosynthesis and starch concentration in whole seedlings of either species in the first year. In the second year, photosynthesis increased in both species in response to the 1991 defoliation treatment, and in red pine also increased in response to the 1990 defoliation treatment. Further, in 1991 both larch and pine had decreased whole-seedling total non-structural carbohydrate concentrations in all seedlings that were defoliated at least once over the 2-yr period. This decrease was noted mostly in the starch component of the nonstructural carbohydrates, and was similar in both species. In 1991, biomass was similarly decreased in both species in response to 1991 defoliation. Both species showed overcompensation in total and component biomass in seedlings defoliated by 25% in 1990. Overall, the results do not support the widely held belief that evergreen trees are substantially more affected than deciduous trees by defoliation.

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“…Total nonstructural carbohydrate (TNC) concentrations were determined using a modification of the method described by Haissig & Dickson (1979) and Hansen & Møller (1975). Sugars were extracted from oven-dried (65 mC, 48 h) leaf powder in methanol-chloroform-water, and tissue residuals were used for starch content determination.…”

Section: Leaf Phenols Nitrogen and Carbohydratesmentioning

confidence: 99%

Primary and secondary host plants differ in leaf‐level photosynthetic response to herbivory: evidence from Alnus and Betula grazed by the alder beetle, Agelastica alni

et al. 1998

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Field-grown trees of Alnus incana (L.) Moench, Alnus glutinosa (L.) Geartner and Betula pendula Roth displayed pronounced differences in responses of light-saturated net photosynthesis (A sat ) to herbivory by the alder beetle (Agelastica alni L., Galerucinae), a specialized insect which primarily defoliates alders. We found that photosynthetic rates of grazed leaves increased following herbivory in Alnus but not in Betula. Area-and massbased A sat of grazed leaves declined linearly with increasing amount of leaf perforation in B. pendula, by as much as 57 %. By contrast Alnus glutinosa and Alnus incana increased area-based rates of A sat by 10-50 % at all levels of leaf grazing. Given increased A sat in the remaining portion of grazed leaves, a net reduction in photosynthesis per leaf occurred only when the proportion of leaf area grazed exceeded 40 % for Alnus incana and 23 % for Alnus glutinosa. Since vein perforation by Agelastica alni was observed much more frequently in leaves of Betula than in Alnus, we hypothesized that declining A sat in herbivorized Betula was related to this disruption of water transport. A field experiment with artificial leaf perforation demonstrated a greater decline in A sat in veinperforated Betula leaves than perforated leaves with midrib veins intact. However, regardless of leaf perforation regime, birch never showed post-perforation increases in A sat . In all species, rates of transpiration of grazed leaves linearly increased and water-use efficiency decreased with increased amount of leaf perforation. In grazed Alnus incana leaves, increasing leaf area consumption by Agelastica alni resulted in an increase of total phenols, a reduction in starch content and no changes in nitrogen concentration in the remaining portion. The increase in photosynthesis in Alnus incana might be related to declining leaf starch concentration or increasing stomatal conductance, but was unrelated to leaf nitrogen concentration. These gas exchange and leaf chemistry measurements suggest that in contrast to B. pendula, Alnus incana and Alnus glutinosa, which are the major host species for Agelastica alni, possess leaf-level physiological adaptations and defence mechanisms which can attenuate negative effects of herbivory by the alder leaf-beetle.

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Starch Measurement in Plant Tissue Using Enzymatic Hydrolysis

Cited by 220 publications

References 14 publications

Effect of seed storage temperature on fine root development and mycorrhizal colonization of young Populus nigra seedlings

Effect of seed storage temperature on fine root development and mycorrhizal colonization of young Populus nigra seedlings

The effect of defoliation intensity and history on photosynthesis, growth and carbon reserves of two conifers with contrasting leaf lifespans and growth habits

Primary and secondary host plants differ in leaf‐level photosynthetic response to herbivory: evidence from Alnus and Betula grazed by the alder beetle, Agelastica alni

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