Effect of seed storage temperature on fine root development and mycorrhizal colonization of young Populus nigra seedlings

Mucha, Joanna; Szymańska, Agata; Zadworny, Marcin; Tylkowski, T.; Michalak, Marcin; Suszka, Jan

doi:10.1007/s13595-015-0470-0

Cited by 7 publications

(5 citation statements)

References 41 publications

(49 reference statements)

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“…The seed viability of S. xerophila and S. koreensis decreased significantly within 16 and 20 weeks of storage at 4°C, respectively, while the viability of S. maximowiczii remained unchanged ( Figures 2 , 3 , and 4 ). These results were consistent with those of previous studies that noted the impacts of storage environment (temperature and moisture) and species on seed longevity [ 25 - 27 ] and later seedling growth from stored seeds [ 28 ]. Similar results were observed for S. maximowiczii seeds, where freshly harvested seed initially showed no signs of aging until a threshold time was reached, after which viability was rapidly lost.…”

Section: Discussionsupporting

confidence: 93%

Extended low temperature and cryostorage longevity of Salix seeds with desiccation control

Han

Kim

2019

Open Life Sciences

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Salix xerophila, S. maximowiczii, and S. koreensis are species of willow native to Korea that are important for bioenergy production. However, the native range of these species has narrowed in recent years due to the impact of climate change. Seeds of these Salix species lose viability within 4 weeks at ambient temperature, and within 4 months at -4°C. Preservation techniques are urgently needed to protect these valuable resources. The effects of seed water content (SWC; 3%, 6%, 9%, 12%, 18%, and 24%) and temperature (ambient, 4°C, -18°C, -80°C, and -196°C) on storage stability were investigated for up to 48, 52, or 60 months, depending on species. Optimal storage temperature and SWC varied between species. S. xerophila seed could be stored without deterioration for 60 months with 9% SWC at -80°C, but rapidly lost viability when stored at -18°C. In S. maximowiczii and S. koreensis, 100% and 90% of normal germination, respectively, was maintained with 18% SWC at -18°C or -80°C. Thus, for some Salix species, storage at -18 and -80°C may provide an economical alternative to cryopreservation or medium-term storage for the maintenance of seedbanks or breeding stocks.

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Section: Discussionsupporting

confidence: 93%

Extended low temperature and cryostorage longevity of Salix seeds with desiccation control

Han

Kim

2019

Open Life Sciences

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“…Additionally, seedlings grown from seeds stored at 18% of MC and 20°C were 50% shorter than from those stored at higher MC levels (da Rosa et al 2011). Mucha et al (2015) showed that seed storage temperature influences the root anatomy of black poplar (Populus nigra) and consequently may alter nutrient absorption. Good-quality seedlings with greater height and root collar diameter confer a higher chance of survival in natural conditions (Grossnickle 2012).…”

Section: Pyrus Communismentioning

confidence: 99%

Effect of different conditions of storage on seed viability and seedling growth of six European wild fruit woody plants

Wawrzyniak

Michalak

Chmielarz

2020

Annals of Forest Science

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Key message Malus sylvestris(L.) Mill.,Pyrus communis(L.),Sorbus aucuparia(L.),Prunus avium(L.),Prunus padus(L.), andCornus sanguinea(L.) are related, co-occurring species producingorthodoxseeds. However, we observed differences in their response to storage conditions, such as storage at different seed moisture contents (5%, 8%, and 11%) and/or temperatures (− 3 °C, − 18 °C, and − 196 °C). Severe desiccation to ca. 5% of MC negatively affected seeds ofM. sylvestris. Seeds ofP. aviumwere sensitive to storage in LN or at − 18 °C.S. aucupariaseeds are best stored at − 3 °C, whereasC. sanguineaseeds tolerate desiccation and storage in LN. In general, species with deeper physiological dormancy (S. aucuparia,P. padus, andC. sanguinea) tended to be more tolerant to desiccation and low temperatures. For all species, storage conditions did not affect seedling growth. Context Wild fruit woody species face many threats such as genetic loss, population fragmentation, and alien species; thus, their genetic variability should be preserved. Aims To examine the effect of storage conditions on seed viability and the initial growth of seedlings of six European wild fruit species: Malus sylvestris (L.) Mill., Pyrus communis (L.), Sorbus aucuparia (L.), Prunus avium (L.), Prunus padus (L.), and Cornus sanguinea (L.). Methods Seeds were desiccated to three different levels of moisture content (ca. 5, 8, or 11%) and stored at three different temperatures (− 3°, − 18°, or − 196 °C; liquid nitrogen, LN) for up to 3 years. Germination and seedling emergence tests were performed as well as measurement of seedling growth. Results Desiccation of M. sylvestris seeds from 10.7 to 4.9% significantly lowered germination from 91 to 77% and seedling emergence from 88 to 74%. In P. avium, LN storage significantly inhibited seedling emergence, both in the laboratory and the greenhouse, but did not affect total seed germination. In P. communis, P. padus, and C. sanguinea, neither germination nor seedling emergence was affected by seed storage conditions. There were small or no differences in stem height and root collar diameter in the first year of seedling growth of stored seeds. Conclusion Species with deeper physiological dormancy (S. aucuparia, P. padus, C. sanguinea) tended to be more tolerant of various storage conditions. Seeds of P. padus and C. sanguinea can be stored long term at − 18 °C or in LN at 5–8% MC without losing viability. M. sylvestris and P. avium seeds are sensitive to desiccation below 6% MC or low temperature of storage at − 18 °C or − 196 °C, respectively. We observed that storage conditions had significant influence on germination and seedling emergence but had no effect on seedling growth after the first growing season.

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“…Finally, it is crucial to ensure proper storage conditions that do not exert any deleterious impacts on seedling development. Therefore, in addition to the assessment of germination, other seed and seedling properties should also be more commonly tested such as morphological properties [ 188 ], proteome and metabolome status [ 98 ], and genetic and epigenetic stability [ 96 , 120 , 145 , 189 , 190 ]. Consequently, there is a huge need for new biochemical or molecular markers of seed viability that improve the process of monitoring plant material during storage in gene banks and to help with the understanding of the concomitant aging process occurring in seeds.…”

Section: Discussionmentioning

confidence: 99%

Choosing the Right Path for the Successful Storage of Seeds

Trusiak

Plitta-Michalak

Michalak

2022

Plants

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Seeds are the most commonly used source of storage material to preserve the genetic diversity of plants. However, prior to the deposition of seeds in gene banks, several questions need to be addressed. Here, we illustrate the scheme that can be used to ensure that the most optimal conditions are identified to enable the long-term storage of seeds. The main questions that need to be answered pertain to the production of viable seeds by plants, the availability of proper protocols for dormancy alleviation and germination, seed tolerance to desiccation and cold storage at −20 °C. Finally, it is very important to fully understand the capability or lack thereof for seeds or their explants to tolerate cryogenic conditions. The proper storage regimes for orthodox, intermediate and recalcitrant seeds are discussed.

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Effect of seed storage temperature on fine root development and mycorrhizal colonization of young Populus nigra seedlings

Cited by 7 publications

References 41 publications

Extended low temperature and cryostorage longevity of Salix seeds with desiccation control

Extended low temperature and cryostorage longevity of Salix seeds with desiccation control

Effect of different conditions of storage on seed viability and seedling growth of six European wild fruit woody plants

Choosing the Right Path for the Successful Storage of Seeds

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