Choosing the Right Path for the Successful Storage of Seeds

Trusiak, Magdalena; Plitta-Michalak, Beata Patrycja; Michalak, Marcin

doi:10.3390/plants12010072

Cited by 7 publications

(8 citation statements)

References 171 publications

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“…Additionally, peroxisome‐related mutations are known to impact seed longevity and germination (Carrera et al., 2007; Pan et al., 2019). Most importantly, even for experiments that do not focus on such genotypes, the age of the seed batch and its storage conditions have drastic impact on germination efficacy (Trusiak et al., 2023). Therefore, accounting for germination timepoints when evaluating root length data is crucial to ensure accurate and meaningful results.…”

Section: Resultsmentioning

confidence: 99%

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SPIRO – the automated Petri plate imaging platform designed by biologists, for biologists

Ohlsson,

Leong,

Elander

et al. 2023

The Plant Journal

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SUMMARYPhenotyping of model organisms grown on Petri plates is often carried out manually, despite the procedures being time‐consuming and laborious. The main reason for this is the limited availability of automated phenotyping facilities, whereas constructing a custom automated solution can be a daunting task for biologists. Here, we describe SPIRO, the Smart Plate Imaging Robot, an automated platform that acquires time‐lapse photographs of up to four vertically oriented Petri plates in a single experiment, corresponding to 192 seedlings for a typical root growth assay and up to 2500 seeds for a germination assay. SPIRO is catered specifically to biologists' needs, requiring no engineering or programming expertise for assembly and operation. Its small footprint is optimized for standard incubators, the inbuilt green LED enables imaging under dark conditions, and remote control provides access to the data without interfering with sample growth. SPIRO's excellent image quality is suitable for automated image processing, which we demonstrate on the example of seed germination and root growth assays. Furthermore, the robot can be easily customized for specific uses, as all information about SPIRO is released under open‐source licenses. Importantly, uninterrupted imaging allows considerably more precise assessment of seed germination parameters and root growth rates compared with manual assays. Moreover, SPIRO enables previously technically challenging assays such as phenotyping in the dark. We illustrate the benefits of SPIRO in proof‐of‐concept experiments which yielded a novel insight on the interplay between autophagy, nitrogen sensing, and photoblastic response.

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Section: Resultsmentioning

confidence: 99%

“…The Plant Journal, (2024), 118, 584-600 drastic impact on germination efficacy (Trusiak et al, 2023). Therefore, accounting for germination timepoints when evaluating root length data is crucial to ensure accurate and meaningful results.…”

Section: Spiro Root Growth Assay Compensates For Variations Caused By...mentioning

confidence: 99%

SPIRO – the automated Petri plate imaging platform designed by biologists, for biologists

Ohlsson,

Leong,

Elander

et al. 2023

The Plant Journal

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show abstract

“…Additionally, peroxisome-related mutations are known to impact seed longevity and germination (Carrera et al, 2007; Pan et al, 2019). Most importantly, even for experiments that do not focus on such genotypes, the age of the seed batch and its storage conditions have drastic impact on germination efficacy (Trusiak et al, 2023). Therefore, accounting for germination timepoints when evaluating root length data is crucial to ensure accurate and meaningful results.…”

Section: Resultsmentioning

confidence: 99%

SPIRO – the automated Petri plate imaging platform designed by biologists, for biologists

Ohlsson

Leong

Elander

et al. 2021

Preprint

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The imaging of plant seedlings, fungal mycelia and bacterial colonies grown on Petri plates is commonly used in phenotyping assays, and is typically done manually despite the procedures being time-consuming and laborious. The main reason for this is the still limited availability of existing automated phenotyping tools and facilities. Additionally, constructing a custom-made automated solution is a daunting task for most research groups specializing in biology. Here, we describe SPIRO, the Smart Plate Imaging Robot, an automated platform that acquires time-lapse photos of up to four vertically oriented Petri plates in a single experiment. SPIRO was designed for biologists by biologists; thus its assembly does not require experience in engineering or programming and its operation is sufficiently intuitive to be done without training. SPIRO has a small footprint optimal to fit into standard incubators for plants and microbes and is equipped with an LED light source for imaging in the dark, thus allowing acquisition of photos under optimal growth conditions. SPIRO's web-based user interface allows setting up experiments and downloading data remotely, without interfering with samples growth. The robots' 8 MP camera provides excellent image quality suitable for automated image processing, which we demonstrate on the example of two semi-automated assays for analysis of commonly used phenotypic traits: seed germination and root growth. Moreover, the robot can be easily customized for a specific use, as all information about SPIRO, including the models for 3D-printed structural components, control software, and scripts for image analysis are released under permissive open source licenses.

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“…Conservation of seeds is the primary method of choice when attempting to preserve the gene pool of a plant species [ 20 , 30 , 33 , 34 , 35 , 36 ]; therefore, deterioration of seeds during storage has profound influences on agriculture and conservation efforts [ 37 ]. Seed aging processes resulting in viability decline and final decrease of plantlet amount are dependent on storage conditions, which are the temperature and RH that determine seed MC.…”

Section: Discussionmentioning

confidence: 99%

“…Currently, seeds of common beech are classified as intermediate [ 15 , 16 , 17 ]. Seeds from this category do not represent either orthodox or recalcitrant type of postharvest behavior; they tolerate desiccation but cannot survive dehydration below those in equilibrium with about 40–50% of relative humidity (RH) and are sensible to subzero temperatures or combined effect of drying and cooling [ 18 , 19 , 20 ]. Although these seeds were found to remain highly viable during 8 years of storage at 5.4% MC and −8.15 °C (265 K) and are assigned as orthodox [ 21 , 22 ], other investigations presented contradictory results showing beech seeds appointing the optimal storage conditions as 7.8–11% MC at −10 °C to −20 °C [ 9 , 16 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Identification of DNA Methylation Changes in European Beech Seeds during Desiccation and Storage

Michalak

Plitta-Michalak

Suszka

et al. 2023

IJMS

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Ageing and deterioration of seeds is a major problem for the maintenance of seed quality and viability during long-term storage. Prediction of early stages of seed deterioration in order to point out the plantlets’ regeneration time is a major challenge of successful storage. In preserved seeds, damages accumulate within cells at the rate mainly related to their moisture content and temperature of storage. Current research reveals global alterations in DNA methylation in lipid-rich intermediate seeds during desiccation and storage at various regimes covering nonoptimal and optimal conditions. We show for the first time that monitoring of 5-methylcytosine (m5C) level in seeds can be used as a truly universal viability marker regardless of postharvest category of seeds and their composition. For seeds stored up to three years, in varied conditions, moisture content, temperature, and time of storage had significant influence on seedling emergence and DNA methylation (p < 0.05). Similarities among lipid-rich intermediate and orthodox seeds regarding different reactions of embryonic axes and cotyledons to desiccation are newly revealed. Along with previous studies on seeds dramatically different in desiccation tolerance (recalcitrant vs. orthodox), results regarding lipid-rich seeds positioned in-between (intermediate) prove that maintaining global DNA methylation status is crucial for maintaining seed viability.

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Choosing the Right Path for the Successful Storage of Seeds

Cited by 7 publications

References 171 publications

SPIRO – the automated Petri plate imaging platform designed by biologists, for biologists

SPIRO – the automated Petri plate imaging platform designed by biologists, for biologists

SPIRO – the automated Petri plate imaging platform designed by biologists, for biologists

Identification of DNA Methylation Changes in European Beech Seeds during Desiccation and Storage

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