Staphylococcus simulans: An emerging cutaneous pathogen

Shields, Bridget E.; Tschetter, Amanda J.; Wanat, Karolyn A.

doi:10.1016/j.jdcr.2016.08.015

Cited by 29 publications

(24 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…S . simulans is a coagulase‐negative staphylococci species that is also considered a skin commensal, but is increasingly implicated in hospital‐acquired cutaneous infection . We also selected two species that are considered wound pathogens: S .…”

Section: Resultsmentioning

confidence: 99%

“…33 S. simulans is a coagulase-negative staphylococci species that is also considered a skin commensal, but is increasingly implicated in hospital-acquired cutaneous infection. 34 We also selected two species that are considered wound pathogens: S. aureus (Subject 171) and P. aeruginosa (Subject 111). The bacterial isolates obtained from each subject are summarized in Figure 3.…”

Section: Identification Of Dfu-isolated Pathogensmentioning

confidence: 99%

See 1 more Smart Citation

Human macrophage response to microbial supernatants from diabetic foot ulcers

Deusenbery

Kalan

Meisel

et al. 2019

Wound Repair Regeneration

View full text Add to dashboard Cite

Diabetic foot ulcers (DFUs) are a major clinical problem exacerbated by prolonged bacterial infection. Macrophages, the primary innate immune cells, are multifunctional cells that regulate diverse processes throughout multiple phases of wound healing. To better understand the influence of microbial species on macrophage behavior, we cultured primary human monocyte‐derived macrophages from four donors for 24 hours in media conditioned by bacteria and fungi (Pseudomonas aeruginosa, Corynebacterium amycolatum, Corynebacterium striatum, Staphylococcus aureus, Staphylococcus simulans, and Candida albicans) isolated from the DFUs of six patients. The effects of these microbe‐derived signals on macrophage behavior were assessed by measuring the gene expression of a panel of 25 genes related to macrophage phenotype, angiogenesis, bacterial recognition, and cell survival, as well as secretion of two inflammatory cytokines using NanoString multiplex analysis. Principal component analysis showed that macrophage gene expression and protein secretion were affected by both microbial species as well as human donor. S. simulans and C. albicans caused up‐regulation of genes associated with a proinflammatory (M1) phenotype, and P. aeruginosa caused an increase in the secretion of the proinflammatory cytokine and M1 marker tumor necrosis factor‐alpha (TNFα). Together, these results suggest that macrophages respond to secreted factors from microbes by up‐regulating inflammatory markers, and that the effects are strongly dependent on the monocyte donor. Ultimately, increased understanding of macrophage–microbe interactions will lead to the development of more targeted therapies for DFU healing.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Identification Of Dfu-isolated Pathogensmentioning

confidence: 99%

Human macrophage response to microbial supernatants from diabetic foot ulcers

Deusenbery

Kalan

Meisel

et al. 2019

Wound Repair Regeneration

View full text Add to dashboard Cite

show abstract

“…Staphylococcus simulans was found to be resistant to the all b-lactams and quinolones. It was isolated by Shields et al as a skin associated pathogens [46], however, sparsely data is available about the antimicrobial susceptibility of this emerging pathogen. We didn't recover S. saprophyticus although it is associated with community acquired urinary tract infections, secondly after Escherichia coli [43,47].…”

Section: Discussionmentioning

confidence: 99%

Incidence and susceptibility patterns of urine bacterial flora in young females

Shami¹,

Al-Mijalli²,

Somily

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: It has been established that the urinary tract is not sterile, however, research related to the study of urine bacteria are limited. Our work aims to study the frequency and patterns of resistance of normal urinary aerobic bacterial flora.Methods: Clean catch midstream urine specimens were collected from 120 young healthy females, and then cultured. Identification of bacteria and antimicrobial susceptibility were done by means of Biomérieux VITEK® 2 automated systems.Subjects who have undergone antimicrobial treatment in one month weren't included.Results: The incidence of positive bacterial cultures was 54.2%, of which 21.5% were ploymicrobial. 107 bacterial isolates that encompass 12 genera and 27 species that were predominated by Gram-positive bacteria (72%) were cultivated. Staphylococcaceae (46.1%) and Enterobacteriaceae (17.8%) were the most frequently isolates among Gram positive and Gram negative bacteria respectively, from them 36 species have been identified as b lactamase producers. The top four frequently isolated bacteria are Micrococcus species (16%), Staphylococcus haemolyticus (13.2%), Staphylococcus aureus (10%), and Klebsiella pneumoniae (10%). Twenty two bacterial species weresubjected to antimicrobial susceptibility testing by using broad and narrow spectrum antibiotics and antimicrobials. Ampicillin showed the lowest susceptibility rate against Gram positives, followed by erythromycin and azithromycin. The lesser antimicrobial susceptibility potential among Gram negative bacteria was exhibited against ampicillin, followed by piperacillin and cefotaxime. Conclusion:Our findings emphasize the importance of highlighting urine bacterial flora in research especially those related to susceptibility patterns, employing more advanced culture methods as multiple drug resistant bacteria were isolated.

show abstract

“…PVL binds to complement receptors on the membranes of these cells and induces membrane channel formation leading to cell destruction. The toxin also induces the release of pro-inflammatory cytokines and nuclear factor-kappa B in neutrophils and is an important virulence factor in necrotizing infections [2]. In PVLPSA pneumonia the risk of death has been reported to be higher than with non-PVL-producing S. aureus (PVLNSA) [3].…”

Section: ) What Is Panton-valentine Leucocidin (Pvl)?mentioning

confidence: 99%

“…In PVLPSA pneumonia the risk of death has been reported to be higher than with non-PVL-producing S. aureus (PVLNSA) [3]. PVL has also been identified in S. haemolyticus and S. simulans [2].…”

Section: ) What Is Panton-valentine Leucocidin (Pvl)?mentioning

confidence: 99%