Staphylococcal food poisoning caused by Staphylococcus argenteus harboring staphylococcal enterotoxin genes

Wakabayashi, Yuki; Umeda, Keiji; Yonogi, Shinya; Nakamura, Hiromi; Yamamoto, Kaori; Kumeda, Yuko; Kawatsu, Kentaro

doi:10.1016/j.ijfoodmicro.2017.10.022

Cited by 63 publications

(50 citation statements)

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“…Since the discovery of S. argenteus in 2009, a lot of the research has focused on distribution in the population [2][3][4][5][6], prevalence rates [4,6,8], presence of virulence genes [3,7,[15][16][17][18]and challenges in clinical diagnostics as compared to S. aureus, while information on S. schweizeri is more scarce. This is the first molecular study on the impact of S. argenteus and S. schweitzeri on human cells in vitro and describes a remarkable cytotoxic effect not seen with any other Staphylococcus species tested here.…”

Section: Discussionmentioning

confidence: 99%

“…S. argenteus and S. schweitzeri have been shown to carry several toxin genes, such as staphylococcal enterotoxins sea, sec, and sed, hemolysins hla and hlb and Panton-Valentine leucocidin (pvl) [3,7,[15][16][17][18], although some studies have indicated a lower prevalence of these genes compared to S. aureus [3,17]. Using S. aureus-specific PCR primers, the majority of S. argenteus isolates were first shown to be PVLnegative [3].…”

Section: Introductionmentioning

confidence: 99%

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Staphylococcus argenteus and Staphylococcus schweitzeri are cytotoxic to human cells in vitro due to high expression of alpha-hemolysin Hla

2019

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Staphylococcus argenteus and Staphylococcus schweitzeri are newly identified species of the S. aureus -related complex. S. argenteus, as occurring globally and showing significant prevalence and comparable infection and morbidity rates compared to S. aureus , is becoming clinically important. Whole genome sequencing has revealed the presence of several virulence genes but the molecular mechanisms of S. argenteus infection and virulence are largely unknown. Here, we studied the effect of a previously characterized clinical S. argenteus isolate on human cells in vitro . The clinical isolate, together with the S. argenteus type strain MSHR1132T and the S. schweitzeri type strain FSA084T, had a cytotoxic effect on the cells, which showed necrotic cell death after a few hours of treatment. The protein causing the cytotoxic effect was purified and identified by mass spectrometry as alpha-hemolysin, Hla, which is awell-known pore-forming toxin in S.aureus . The cytotoxic effect could be blocked with an antibody against Hla. S.argenteus showed 12–15 fold higher expression levels of hla at the RNA level and 4–6 fold higher expression levels at the protein level compared to S.aureus . The higher expression levels of hla were supported by higher RNA levels of the regulatory factors sarA and saeR . Also, the RNAIII component of the accessory gene regulator (agr) quorum sensing system was 8,000–10,000 fold higher in the S.argenteus isolates compared to S.aureus . This is the first study on the effect of S.argenteus on ahuman cell line and strengthens the idea of significant virulence of S.argenteus .

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Staphylococcus argenteus and Staphylococcus schweitzeri are cytotoxic to human cells in vitro due to high expression of alpha-hemolysin Hla

2019

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“…Staphylococcus spp. has also been implicated in staphylococcal foodborne disease caused by ingesting food containing staphylococcal enterotoxins (Wakabayashi et al, 2018). Staphylococcus sp.…”

Section: Discussionmentioning

confidence: 99%

Microbiology, heterocyclic amines and polycyclic aromatic hydrocarbons profiles of some grilled, roasted and smoked foods in Lagos and Ogun States, Nigeria

Oranusi¹,

Onibokun²,

Obafemi³

et al. 2018

Afr. J. Food Sci.

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Dietary intake of polycyclic aromatic hydrocarbons (PAH) and heterocyclic amines (HCA) has posted a great health risk as they have been identified as a most potent human carcinogen. Microbial quality of food is also of concern as they contribute to food poisoning and infection. Sixty food samples comprising roasted yam, plantain, grilled and smoked fish and meat were randomly sampled from Lagos and Ogun State, Nigeria, and the PAHs, HCAs contents and microbial load were determined. Isolates were subjected to antibiogram assay. The pH of the samples ranged between 5.08 and 7.49, titrable acidity was in the range of 0.50 and 1.20. Staphylococcus, Bacillus, Micrococcus, Proteus, Pseudomonas, Citrobacter and Klebsiella sp. had been identified. Antibiogram revealed that the isolates were multi-resistant and most resistant to ceftazidime, cloxacillin and tetracycline and more susceptible to ofloxacin. PAHs were detected in some grilled, roasted and smoked samples and with the highest concentrations 314.85 and 139.97 µg/g Dibenzene[a,h]anthracene established in roasted yam and smoked fish samples. Only 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) was detected in grilled fish and meat samples. This study therefore established the presence of chemical and microbial contaminants in some of the food items investigated. It recommended that strict sanitary practices and appropriate cooking methods be enforced during food preparation.

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“…poisoning in spite of its commensal presence in humans being, an etiological transporter of foodborne diseases. Infection acquired from hospital scenario outrages community-acquired ones 5 . There are more than 50 species and subspecies of Staphylococci of which S. aureus is often associated with pathogenicity in humans.…”

Section: Introductionmentioning

confidence: 99%

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2019

IJPSR

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Staphylococcus aureus, the facultative anaerobic and opportunistic Bacterium has been characterized as the pathogen with diversified molecular adaptability leading to the emergence of MDR. Advanced use of alternate antibiotics is increasing the scenario of the emergence of Methicillin-Resistant Staphylococcus aureus (MRSA) to Oxacillin Resistant Staphylococcus aureus (ORSA) after that generating associated health infections. Even though Staphylococcus aureus is being considered as one of the important pathogens for its combination of virulence including toxin-mediated multidrug resistance and invasiveness, staphylococcus food poisoning is of high on interest as this infection rate is increasing day by day. Bacteriophage therapy has been of interest due to the absence of secondary resistance and targeted attack and also the lack of recolonization of the pathogen. The present study aims to isolate and purify Staphylococcus aureus-specific bacteriophages from sewage samples and thereby expunging food poison, causing Staphylococcus aureus. On this aspect Staphylococcus aureus causing food poison were isolated from a stool sample of patients at hospitals, and the specific Bacteriophage was isolated from the sewage in Tirunelveli District, India. Enrichment technique improved the stability of the phages. Spot and plaque assay experiments were done to examine the specificity of the isolated phage. Techniques employed result in providing purified specific bacteriophage for Staphylococcus aureus.

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Staphylococcal food poisoning caused by Staphylococcus argenteus harboring staphylococcal enterotoxin genes

Cited by 63 publications

References 23 publications

Staphylococcus argenteus and Staphylococcus schweitzeri are cytotoxic to human cells in vitro due to high expression of alpha-hemolysin Hla

Staphylococcus argenteus and Staphylococcus schweitzeri are cytotoxic to human cells in vitro due to high expression of alpha-hemolysin Hla

Microbiology, heterocyclic amines and polycyclic aromatic hydrocarbons profiles of some grilled, roasted and smoked foods in Lagos and Ogun States, Nigeria

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