Staphylococcal cassette chromosome mec (SCCmec) characterization and molecular analysis for methicillin-resistant Staphylococcus aureus and novel SCCmec subtype IVg isolated from bovine milk in Korea

Kwon, Nam Hoon; Park, Kun Taek; Moon, Jin San; Jung, Woo Kyung; Kim, So Hyun; Kim, Jun Man; Hong, Soon Keun; Koo, Hye Cheung; Joo, Yi-Seok; Park, Taesung

doi:10.1093/jac/dki306

Cited by 145 publications

(102 citation statements)

References 34 publications

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“…Therefore, the MRSA isolate was assumed to be a hospital-derived clone introduced into the community. However, an SCCmec IV clone has been found in community settings such as in neonates born at primary obstetrics clinics (26), in a surveillance of healthy schoolchildren (30), and in cases of bovine mastitis (27). As was the case in this study, such SCCmec IV clones were pUB110 positive and of type IVA and did not show multidrug resistance (5,26).…”

Section: Discussionsupporting

confidence: 55%

Epidemiological Characteristics of Methicillin-Resistant Staphylococcus aureus Isolates from Children with Eczematous Atopic Dermatitis Lesions

Chung¹,

Jeon²,

Sung³

et al. 2008

J Clin Microbiol

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In this study, we investigated the rate of colonization of skin of children with atopic dermatitis (AD) by methicillin-resistant Staphylococcus aureus (MRSA) and characterized the isolates. Active skin lesions in pediatric AD patients were cultured with Rodac Staph (Komed, Korea). S. aureus isolates were examined for drug susceptibilities, analyzed for the eta, etb, tst, and pvl genes, and typed using agr polymorphism, pulsed-field gel electrophoresis of SmaI-restricted chromosomal DNA, and staphylococcal cassette chromosome mec (SCCmec) typing. Eighty-seven (75.4%) of 115 patients had cultivable S. aureus isolates, 16 of which (18.3%) were MRSA. All MRSA isolates were susceptible to chloramphenicol, rifampin, cotrimoxazole, and ciprofloxacin. While methicillin-susceptible S. aureus (MSSA) isolates were composed of 23 isolates of singular types and nine clusters comprising 48 isolates, MRSA isolates were typed into three clones: eight isolates of pulsotype A-agr-1-SCCmec IV, five isolates of pulsotype B-agr-3-SCCmec IIb-etb positive, and three isolates of pulsotype C-agr-3-SCCmec IV. Three SCCmec IVA MRSA isolates were tst positive, but none were positive for the pvl or eta gene. Among 71 MSSA isolates, 7 isolates were tst positive, 6 of which were pulsotype F-agr-3, and 9 of 10 agr-4 isolates were eta positive. The average ages of patients carrying MSSA, SCCmec IVA MRSA, and SCCmec IIb MRSA were 7.7 ؎ 4.6, 3.1 ؎ 1.5, and 8.2 ؎ 3.1 years, respectively. Among the patients carrying MRSA, two patients had been treated with oral antimicrobials, and one had been admitted to the hospital 18 months previously. In conclusion, community-acquired MRSA isolates of a few clones colonized the skin of patients with AD without risk factors for the acquisition of hospital-acquired MRSA, which suggested that the skin of children with AD may represent a significant reservoir of MRSA colonization in the community.

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Section: Discussionsupporting

confidence: 55%

Epidemiological Characteristics of Methicillin-Resistant Staphylococcus aureus Isolates from Children with Eczematous Atopic Dermatitis Lesions

Chung¹,

Jeon²,

Sung³

et al. 2008

J Clin Microbiol

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“…Caution should be exercised in regard to raw milk ingestion, which is a common practice in rural areas under development. However, further studies for the purpose of determining the real importance of MRSA from animal-derived foods on public health issues are required, since MRSA isolated from food animals do not necessarily correspond genetically to those clones linked to human infections (Kwon et al 2005).…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial resistance of Staphylococcus spp. from small ruminant mastitis in Brazil

et al. 2012

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Pesq. Vet. Bras. 32(8) The study aimed to determine the antimicrobial resistance patterns and to identify molecular resistance markers in Staphylococcus spp. (n=210) isolated from small ruminant mastitis in Brazil. The antimicrobial resistance patterns were evaluated by the disk diffusion test and by detection of the presence of mecA, blaZ, ermA, ermB, ermC and msrA genes by PCR. The efϐlux pump test was performed using ethidium bromide and bioϐilm production was determined by Congo red agar test along with PCR for detection of the icaD gene. The isolates were most resistant to amoxicillin (50.0%), streptomycin (42.8%), tetracycline (40.4%), lincomycin (39.0%) and erythromycin (33.8%). Pan-susceptibility to all tested drugs was observed in 71 (33.8%) isolates and 41 Staphylococcus isolates were positive for the efϐlux pump. Although phenotypic resistance to oxacillin was observed in 12.8% of the isolates, none harbored the mecA gene. However, 45.7% of the isolates harbored blaZ indicating that beta-lactamase production was the main mechanism associated with staphylococci resistance to beta-lactams in the present study. The other determinants of resistance to antimicrobial agents ermA, ermB, ermC, and msrA were observed in 1.4%, 10.4%, 16.2%, and 0.9% of the isolates, respectively. In addition, the icaD gen was detected in 32.9% of the isolates. Seventy three isolates (54 from goats and 19 from sheep) were negative for all resistance genes tested and 69 isolates presented two or more resistance genes. Association among blaZ, ermA, ermB, ermC and efϐlux pump were observed in 17 isolates, 14 of which originated from goats and three from sheep. The data obtained in this study show the resistance of the isolates to beta-lactamics, which may be associated with the use of antimicrobial drugs without veterinary control.

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“…Its potential for transmission between humans has also been observed in the Netherlands (4,7). MRSA has been isolated from dairy products, beef, chicken and pork (33)(34)(35)(36)(37) and although foodborne transmission is plausible (34), the risk is thought to be low. Preference for eating pork was not associated with being a CC398 case-patient in our study.…”

Section: Discussionmentioning

confidence: 99%

Pigs as Source of Methicillin-ResistantStaphylococcus aureusCC398 Infections in Humans, Denmark

Lewis¹,

Mølbak²,

Reese³

et al. 2008

Emerg. Infect. Dis.

242

189

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An emerging subtype of methicillin-resistant Staphylococcus aureus (MRSA), clonal complex (CC) 398, is associated with animals, particularly pigs. We conducted a matched case-control and a case-case study comparing 21 CC398 case-patients with 2 controls randomly selected from the Danish Civil Registry and 2 case-patients infected with MRSA other than CC398. On farms of case-patients, animals were examined for MRSA. Thirteen case-patients reported pig exposure. Living or working on farms with animals was an independent risk factor for CC398 in the casecontrol (matched odds ratio [MOR] 35.4, 95% confi dence interval [CI] 2.7-469.8) and the case-case study (MOR 14.5, 95%CI 2.7-76.7). History of hospitalization was associated with an increased risk only in the case-control study (MOR 11.4,. A total of 23 of 50 pigs on 4 of 5 farms were positive for CC398. Our results, corroborated by microbiologic testing, demonstrate that pigs are a source of CC398 in Denmark.

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Staphylococcal cassette chromosome mec (SCCmec) characterization and molecular analysis for methicillin-resistant Staphylococcus aureus and novel SCCmec subtype IVg isolated from bovine milk in Korea

Abstract: MRSA isolated from bovine milk harboured a unique SCCmec subtype, and they may not be correlated with the emergence of CA-MRSA in human infection in Korea.

Cited by 145 publications

References 34 publications

Epidemiological Characteristics of Methicillin-Resistant Staphylococcus aureus Isolates from Children with Eczematous Atopic Dermatitis Lesions

Epidemiological Characteristics of Methicillin-Resistant Staphylococcus aureus Isolates from Children with Eczematous Atopic Dermatitis Lesions

Antimicrobial resistance of Staphylococcus spp. from small ruminant mastitis in Brazil

Pigs as Source of Methicillin-ResistantStaphylococcus aureusCC398 Infections in Humans, Denmark

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